LOCUS       V014078                13333 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014078
VERSION     V014078
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2589..2829
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2839..2874
                     /label="RfxCas13d DR36"
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA
                     (Konermann et al., 2018)"
     promoter        3024..4202
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             4221..4241
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             4251..7148
                     /label="RfxCas13d"
                     /note="RNA-targeting Type VI CRISPR protein from
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
     CDS             7158..7178
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             7188..7214
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             7284..7997
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    8025..8613
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    9350..9574
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9620..10000
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10062..10391
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        10439..10486
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             10505..10876
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    11009..11142
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(11227..11257)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11272..11293)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11581..12169)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12343..13200)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13201..13305)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggta atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     2641 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2701 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2761 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2821 ggaaaggacg aaacaccgca agtaaacccc taccaactgg tcggggtttg aaacggagac
     2881 ggacgtctct caagtaaacc cctaccaact ggtcggggtt tgaaactttt tttggttaat
     2941 taatgcaaag atggataaag ttttaaacag agaggaatct tttcagctaa tggaccttct
     3001 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3061 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3121 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3181 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3241 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3301 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     3361 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     3421 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     3481 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     3541 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     3601 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     3661 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     3721 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     3781 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     3841 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     3901 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     3961 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     4021 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     4081 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     4141 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     4201 gacgtacggc caccatgagc cccaagaaga agagaaaggt ggaggccagc atcgaaaaaa
     4261 aaaagtcctt cgccaagggc atgggcgtga agtccacact cgtgtccggc tccaaagtgt
     4321 acatgacaac cttcgccgaa ggcagcgacg ccaggctgga aaagatcgtg gagggcgaca
     4381 gcatcaggag cgtgaatgag ggcgaggcct tcagcgctga aatggccgat aaaaacgccg
     4441 gctataagat cggcaacgcc aaattcagcc atcctaaggg ctacgccgtg gtggctaaca
     4501 accctctgta tacaggaccc gtccagcagg atatgctcgg cctgaaggaa actctggaaa
     4561 agaggtactt cggcgagagc gctgatggca atgacaatat ttgtatccag gtgatccata
     4621 acatcctgga cattgaaaaa atcctcgccg aatacattac caacgccgcc tacgccgtca
     4681 acaatatctc cggcctggat aaggacatta ttggattcgg caagttctcc acagtgtata
     4741 cctacgacga attcaaagac cccgagcacc atagggccgc tttcaacaat aacgataagc
     4801 tcatcaacgc catcaaggcc cagtatgacg agttcgacaa cttcctcgat aaccccagac
     4861 tcggctattt cggccaggcc tttttcagca aggagggcag aaattacatc atcaattacg
     4921 gcaacgaatg ctatgacatt ctggccctcc tgagcggact gaggcactgg gtggtccata
     4981 acaacgaaga agagtccagg atctccagga cctggctcta caacctcgat aagaacctcg
     5041 acaacgaata catctccacc ctcaactacc tctacgacag gatcaccaat gagctgacca
     5101 actccttctc caagaactcc gccgccaacg tgaactatat tgccgaaact ctgggaatca
     5161 accctgccga attcgccgaa caatatttca gattcagcat tatgaaagag cagaaaaacc
     5221 tcggattcaa tatcaccaag ctcagggaag tgatgctgga caggaaggat atgtccgaga
     5281 tcaggaaaaa tcataaggtg ttcgactcca tcaggaccaa ggtctacacc atgatggact
     5341 ttgtgattta taggtattac atcgaagagg atgccaaggt ggctgccgcc aataagtccc
     5401 tccccgataa tgagaagtcc ctgagcgaga aggatatctt tgtgattaac ctgaggggct
     5461 ccttcaacga cgaccagaag gatgccctct actacgatga agctaataga atttggagaa
     5521 agctcgaaaa tatcatgcac aacatcaagg aatttagggg aaacaagaca agagagtata
     5581 agaagaagga cgcccctaga ctgcccagaa tcctgcccgc tggccgtgat gtttccgcct
     5641 tcagcaaact catgtatgcc ctgaccatgt tcctggatgg caaggagatc aacgacctcc
     5701 tgaccaccct gattaataaa ttcgataaca tccagagctt cctgaaggtg atgcctctca
     5761 tcggagtcaa cgctaagttc gtggaggaat acgccttttt caaagactcc gccaagatcg
     5821 ccgatgagct gaggctgatc aagtccttcg ctagaatggg agaacctatt gccgatgcca
     5881 ggagggccat gtatatcgac gccatccgta ttttaggaac caacctgtcc tatgatgagc
     5941 tcaaggccct cgccgacacc ttttccctgg acgagaacgg aaacaagctc aagaaaggca
     6001 agcacggcat gagaaatttc attattaata acgtgatcag caataaaagg ttccactacc
     6061 tgatcagata cggtgatcct gcccacctcc atgagatcgc caaaaacgag gccgtggtga
     6121 agttcgtgct cggcaggatc gctgacatcc agaaaaaaca gggccagaac ggcaagaacc
     6181 agatcgacag gtactacgaa acttgtatcg gaaaggataa gggcaagagc gtgagcgaaa
     6241 aggtggacgc tctcacaaag atcatcaccg gaatgaacta cgaccaattc gacaagaaaa
     6301 ggagcgtcat tgaggacacc ggcagggaaa acgccgagag ggagaagttt aaaaagatca
     6361 tcagcctgta cctcaccgtg atctaccaca tcctcaagaa tattgtcaat atcaacgcca
     6421 ggtacgtcat cggattccat tgcgtcgagc gtgatgctca actgtacaag gagaaaggct
     6481 acgacatcaa tctcaagaaa ctggaagaga agggattcag ctccgtcacc aagctctgcg
     6541 ctggcattga tgaaactgcc cccgataaga gaaaggacgt ggaaaaggag atggctgaaa
     6601 gagccaagga gagcattgac agcctcgaga gcgccaaccc caagctgtat gccaattaca
     6661 tcaaatacag cgacgagaag aaagccgagg agttcaccag gcagattaac agggagaagg
     6721 ccaaaaccgc cctgaacgcc tacctgagga acaccaagtg gaatgtgatc atcagggagg
     6781 acctcctgag aattgacaac aagacatgta ccctgttcag aaacaaggcc gtccacctgg
     6841 aagtggccag gtatgtccac gcctatatca acgacattgc cgaggtcaat tcctacttcc
     6901 aactgtacca ttacatcatg cagagaatta tcatgaatga gaggtacgag aaaagcagcg
     6961 gaaaggtgtc cgagtacttc gacgctgtga atgacgagaa gaagtacaac gataggctcc
     7021 tgaaactgct gtgtgtgcct ttcggctact gtatccccag gtttaagaac ctgagcatcg
     7081 aggccctgtt cgataggaac gaggccgcca agttcgacaa ggagaaaaag aaggtgtccg
     7141 gcaattccgg atccggacct aagaaaaaga ggaaggtggc ggccgcttac ccatacgatg
     7201 ttccagatta cgctgctagc ggcagtggag agggcagagg aagtctgcta acatgcggtg
     7261 acgtcgagga gaatcctggc ccagtgagca agggcgagga gctgttcacc ggggtggtgc
     7321 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     7381 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     7441 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     7501 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     7561 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     7621 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     7681 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     7741 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     7801 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     7861 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     7921 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     7981 tggacgagct gtacaagtaa gaattcgata tcaagcttat cgataatcaa cctctggatt
     8041 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8101 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8161 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8221 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8281 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8341 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8401 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     8461 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8521 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     8581 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     8641 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     8701 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     8761 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8821 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     8881 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     8941 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     9001 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     9061 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     9121 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9181 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9241 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9301 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     9361 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9421 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9481 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9541 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     9601 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10021 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    10081 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    10141 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    10201 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    10261 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10321 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10381 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
    10441 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
    10501 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
    10561 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
    10621 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
    10681 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
    10741 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    10801 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    10861 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    10921 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    10981 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    11041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    11101 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    11161 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    11221 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    11281 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    11341 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    11401 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11461 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11521 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11581 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11641 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11701 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11761 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11821 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11881 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11941 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    12001 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    12061 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12121 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12181 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12241 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    12301 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    12361 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    12421 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12481 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12541 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    12601 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12661 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12721 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12781 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12841 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12901 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12961 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    13021 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    13081 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    13141 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    13201 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    13261 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    13321 agtgccacct gac
//
