LOCUS       V014536                14062 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014536
VERSION     V014536
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2589..2829
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2839..2874
                     /label="RfxCas13d DR36"
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA
                     (Konermann et al., 2018)"
     promoter        3024..4202
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             5010..7907
                     /label="RfxCas13d"
                     /note="RNA-targeting Type VI CRISPR protein from
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
     CDS             7917..7943
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             8013..8726
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    8754..9342
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    10079..10303
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10349..10777
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10791..11120
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        11168..11215
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             11234..11605
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    11738..11871
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(11956..11986)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12001..12022)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12310..12898)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13072..13929)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13930..14034)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggta atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     2641 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2701 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2761 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2821 ggaaaggacg aaacaccgca agtaaacccc taccaactgg tcggggtttg aaacggagac
     2881 ggacgtctct caagtaaacc cctaccaact ggtcggggtt tgaaactttt tttggttaat
     2941 taatgcaaag atggataaag ttttaaacag agaggaatct tttcagctaa tggaccttct
     3001 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3061 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3121 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3181 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3241 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3301 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     3361 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     3421 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     3481 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     3541 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     3601 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     3661 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     3721 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     3781 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     3841 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     3901 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     3961 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     4021 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     4081 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     4141 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     4201 gacgtacggc caccatgagc ggcaagttgt ctgaatcaga gattaggttt ggactgaaaa
     4261 cggaggtgat gggccaacat cttatctatc acgatgtttt gtccagcaca caaaagacag
     4321 cccatgaatt ggcaaataac aatgcccccg agggtaccct cgtggttgca gataaacaga
     4381 cagccggtag gggcgggatg agccgagtgt ggcattctca agaaggaaac ggagtatgga
     4441 tgagtttgat attgcgcccg gacattccgc tccaaaagac gccccaactt accttgctgg
     4501 cagctgtagc ggtggttcag ggaatagaag aggccgcggg catacagact gatataaagt
     4561 ggccgaacga catactcatt aacgggaaaa aaacagtagg gatacttacc gaaatgcaag
     4621 ctgaggagga tcgagtgcgc tccgtgatta ttggtattgg cattaatgta aatcagcaac
     4681 ccaatgattt ccccgatgag ctcaaggaca ttgcgacgag tctgtcccag gcagctgggg
     4741 aaaagatcga cagggcaggt gtaatccagc atatcttgct ttgtttcgaa aagcgatacc
     4801 gagactacat gacccacggt ttcacgccca tcaagctctt gtgggagtca tacgccctcg
     4861 gtattggcac gaacatgagg gcgagaactc tcaacgggac attttacggt aaggcgttgg
     4921 gtattgacga cgaaggggta cttttgttgg aaactaatga aggcattaag aagatataca
     4981 gcgcggacat cagccttagg gaggccagca tcgaaaaaaa aaagtccttc gccaagggca
     5041 tgggcgtgaa gtccacactc gtgtccggct ccaaagtgta catgacaacc ttcgccgaag
     5101 gcagcgacgc caggctggaa aagatcgtgg agggcgacag catcaggagc gtgaatgagg
     5161 gcgaggcctt cagcgctgaa atggccgata aaaacgccgg ctataagatc ggcaacgcca
     5221 aattcagcca tcctaagggc tacgccgtgg tggctaacaa ccctctgtat acaggacccg
     5281 tccagcagga tatgctcggc ctgaaggaaa ctctggaaaa gaggtacttc ggcgagagcg
     5341 ctgatggcaa tgacaatatt tgtatccagg tgatccataa catcctggac attgaaaaaa
     5401 tcctcgccga atacattacc aacgccgcct acgccgtcaa caatatctcc ggcctggata
     5461 aggacattat tggattcggc aagttctcca cagtgtatac ctacgacgaa ttcaaagacc
     5521 ccgagcacca tagggccgct ttcaacaata acgataagct catcaacgcc atcaaggccc
     5581 agtatgacga gttcgacaac ttcctcgata accccagact cggctatttc ggccaggcct
     5641 ttttcagcaa ggagggcaga aattacatca tcaattacgg caacgaatgc tatgacattc
     5701 tggccctcct gagcggactg gcgcactggg tggtcgctaa caacgaagaa gagtccagga
     5761 tctccaggac ctggctctac aacctcgata agaacctcga caacgaatac atctccaccc
     5821 tcaactacct ctacgacagg atcaccaatg agctgaccaa ctccttctcc aagaactccg
     5881 ccgccaacgt gaactatatt gccgaaactc tgggaatcaa ccctgccgaa ttcgccgaac
     5941 aatatttcag attcagcatt atgaaagagc agaaaaacct cggattcaat atcaccaagc
     6001 tcagggaagt gatgctggac aggaaggata tgtccgagat caggaaaaat cataaggtgt
     6061 tcgactccat caggaccaag gtctacacca tgatggactt tgtgatttat aggtattaca
     6121 tcgaagagga tgccaaggtg gctgccgcca ataagtccct ccccgataat gagaagtccc
     6181 tgagcgagaa ggatatcttt gtgattaacc tgaggggctc cttcaacgac gaccagaagg
     6241 atgccctcta ctacgatgaa gctaatagaa tttggagaaa gctcgaaaat atcatgcaca
     6301 acatcaagga atttagggga aacaagacaa gagagtataa gaagaaggac gcccctagac
     6361 tgcccagaat cctgcccgct ggccgtgatg tttccgcctt cagcaaactc atgtatgccc
     6421 tgaccatgtt cctggatggc aaggagatca acgacctcct gaccaccctg attaataaat
     6481 tcgataacat ccagagcttc ctgaaggtga tgcctctcat cggagtcaac gctaagttcg
     6541 tggaggaata cgcctttttc aaagactccg ccaagatcgc cgatgagctg aggctgatca
     6601 agtccttcgc tagaatggga gaacctattg ccgatgccag gagggccatg tatatcgacg
     6661 ccatccgtat tttaggaacc aacctgtcct atgatgagct caaggccctc gccgacacct
     6721 tttccctgga cgagaacgga aacaagctca agaaaggcaa gcacggcatg agaaatttca
     6781 ttattaataa cgtgatcagc aataaaaggt tccactacct gatcagatac ggtgatcctg
     6841 cccacctcca tgagatcgcc aaaaacgagg ccgtggtgaa gttcgtgctc ggcaggatcg
     6901 ctgacatcca gaaaaaacag ggccagaacg gcaagaacca gatcgacagg tactacgaaa
     6961 cttgtatcgg aaaggataag ggcaagagcg tgagcgaaaa ggtggacgct ctcacaaaga
     7021 tcatcaccgg aatgaactac gaccaattcg acaagaaaag gagcgtcatt gaggacaccg
     7081 gcagggaaaa cgccgagagg gagaagttta aaaagatcat cagcctgtac ctcaccgtga
     7141 tctaccacat cctcaagaat attgtcaata tcaacgccag gtacgtcatc ggattccatt
     7201 gcgtcgagcg tgatgctcaa ctgtacaagg agaaaggcta cgacatcaat ctcaagaaac
     7261 tggaagagaa gggattcagc tccgtcacca agctctgcgc tggcattgat gaaactgccc
     7321 ccgataagag aaaggacgtg gaaaaggaga tggctgaaag agccaaggag agcattgaca
     7381 gcctcgagag cgccaacccc aagctgtatg ccaattacat caaatacagc gacgagaaga
     7441 aagccgagga gttcaccagg cagattaaca gggagaaggc caaaaccgcc ctgaacgcct
     7501 acctgaggaa caccaagtgg aatgtgatca tcagggagga cctcctgaga attgacaaca
     7561 agacatgtac cctgttcgca aacaaggccg tcgccctgga agtggccagg tatgtccacg
     7621 cctatatcaa cgacattgcc gaggtcaatt cctacttcca actgtaccat tacatcatgc
     7681 agagaattat catgaatgag aggtacgaga aaagcagcgg aaaggtgtcc gagtacttcg
     7741 acgctgtgaa tgacgagaag aagtacaacg ataggctcct gaaactgctg tgtgtgcctt
     7801 tcggctactg tatccccagg tttaagaacc tgagcatcga ggccctgttc gataggaacg
     7861 aggccgccaa gttcgacaag gagaaaaaga aggtgtccgg caattccgga tccggatacc
     7921 catacgatgt tccagattac gctgctagcg gcagtggaga gggcagagga agtctgctaa
     7981 catgcggtga cgtcgaggag aatcctggcc cagtgagcaa gggcgaggag ctgttcaccg
     8041 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     8101 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     8161 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     8221 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     8281 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     8341 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     8401 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     8461 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     8521 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     8581 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     8641 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     8701 ctctcggcat ggacgagctg tacaagtaag aattcgatat caagcttatc gataatcaac
     8761 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     8821 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     8881 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     8941 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9001 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     9061 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     9121 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     9181 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9241 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     9301 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     9361 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     9421 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     9481 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9541 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     9601 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     9661 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     9721 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     9781 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     9841 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9901 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9961 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10021 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
    10081 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    10141 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    10201 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    10261 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
    10321 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
    10381 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10441 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10501 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10561 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10621 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10681 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10741 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
    10801 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10861 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10921 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10981 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    11041 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    11101 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    11161 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
    11221 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
    11281 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
    11341 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
    11401 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
    11461 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
    11521 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
    11581 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
    11641 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    11701 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    11761 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11821 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    11881 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    11941 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    12001 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    12061 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    12121 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    12181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    12241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    12301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    12361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    12421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    12481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    12541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    12601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    12661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    12721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    12781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    12841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    12901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    13021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    13081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    13141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    13201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    13261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    13321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    13381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    13441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    13501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    13561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    13621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    13681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    13741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    13801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    13861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    13921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    13981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    14041 tccccgaaaa gtgccacctg ac
//
