LOCUS       V017446                12991 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017446
VERSION     V017446
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12991)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2682..3860
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3879..3899
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             3909..6806
                     /label="RfxCas13d"
                     /note="RNA-targeting Type VI CRISPR protein from
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
     CDS             6816..6836
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             6846..6872
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             6942..7655
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    7683..8271
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    9008..9232
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9278..9706
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10097..10144
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             10163..10534
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    10667..10800
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(10885..10915)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10930..10951)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11239..11827)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12001..12858)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12859..12963)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3301 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3481 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3841 ttccatttca ggtgtcgtga cgtacggcca ccatgagccc caagaagaag agaaaggtgg
     3901 aggccagcat cgaaaaaaaa aagtccttcg ccaagggcat gggcgtgaag tccacactcg
     3961 tgtccggctc caaagtgtac atgacaacct tcgccgaagg cagcgacgcc aggctggaaa
     4021 agatcgtgga gggcgacagc atcaggagcg tgaatgaggg cgaggccttc agcgctgaaa
     4081 tggccgataa aaacgccggc tataagatcg gcaacgccaa attcagccat cctaagggct
     4141 acgccgtggt ggctaacaac cctctgtata caggacccgt ccagcaggat atgctcggcc
     4201 tgaaggaaac tctggaaaag aggtacttcg gcgagagcgc tgatggcaat gacaatattt
     4261 gtatccaggt gatccataac atcctggaca ttgaaaaaat cctcgccgaa tacattacca
     4321 acgccgccta cgccgtcaac aatatctccg gcctggataa ggacattatt ggattcggca
     4381 agttctccac agtgtatacc tacgacgaat tcaaagaccc cgagcaccat agggccgctt
     4441 tcaacaataa cgataagctc atcaacgcca tcaaggccca gtatgacgag ttcgacaact
     4501 tcctcgataa ccccagactc ggctatttcg gccaggcctt tttcagcaag gagggcagaa
     4561 attacatcat caattacggc aacgaatgct atgacattct ggccctcctg agcggactga
     4621 ggcactgggt ggtccataac aacgaagaag agtccaggat ctccaggacc tggctctaca
     4681 acctcgataa gaacctcgac aacgaataca tctccaccct caactacctc tacgacagga
     4741 tcaccaatga gctgaccaac tccttctcca agaactccgc cgccaacgtg aactatattg
     4801 ccgaaactct gggaatcaac cctgccgaat tcgccgaaca atatttcaga ttcagcatta
     4861 tgaaagagca gaaaaacctc ggattcaata tcaccaagct cagggaagtg atgctggaca
     4921 ggaaggatat gtccgagatc aggaaaaatc ataaggtgtt cgactccatc aggaccaagg
     4981 tctacaccat gatggacttt gtgatttata ggtattacat cgaagaggat gccaaggtgg
     5041 ctgccgccaa taagtccctc cccgataatg agaagtccct gagcgagaag gatatctttg
     5101 tgattaacct gaggggctcc ttcaacgacg accagaagga tgccctctac tacgatgaag
     5161 ctaatagaat ttggagaaag ctcgaaaata tcatgcacaa catcaaggaa tttaggggaa
     5221 acaagacaag agagtataag aagaaggacg cccctagact gcccagaatc ctgcccgctg
     5281 gccgtgatgt ttccgccttc agcaaactca tgtatgccct gaccatgttc ctggatggca
     5341 aggagatcaa cgacctcctg accaccctga ttaataaatt cgataacatc cagagcttcc
     5401 tgaaggtgat gcctctcatc ggagtcaacg ctaagttcgt ggaggaatac gcctttttca
     5461 aagactccgc caagatcgcc gatgagctga ggctgatcaa gtccttcgct agaatgggag
     5521 aacctattgc cgatgccagg agggccatgt atatcgacgc catccgtatt ttaggaacca
     5581 acctgtccta tgatgagctc aaggccctcg ccgacacctt ttccctggac gagaacggaa
     5641 acaagctcaa gaaaggcaag cacggcatga gaaatttcat tattaataac gtgatcagca
     5701 ataaaaggtt ccactacctg atcagatacg gtgatcctgc ccacctccat gagatcgcca
     5761 aaaacgaggc cgtggtgaag ttcgtgctcg gcaggatcgc tgacatccag aaaaaacagg
     5821 gccagaacgg caagaaccag atcgacaggt actacgaaac ttgtatcgga aaggataagg
     5881 gcaagagcgt gagcgaaaag gtggacgctc tcacaaagat catcaccgga atgaactacg
     5941 accaattcga caagaaaagg agcgtcattg aggacaccgg cagggaaaac gccgagaggg
     6001 agaagtttaa aaagatcatc agcctgtacc tcaccgtgat ctaccacatc ctcaagaata
     6061 ttgtcaatat caacgccagg tacgtcatcg gattccattg cgtcgagcgt gatgctcaac
     6121 tgtacaagga gaaaggctac gacatcaatc tcaagaaact ggaagagaag ggattcagct
     6181 ccgtcaccaa gctctgcgct ggcattgatg aaactgcccc cgataagaga aaggacgtgg
     6241 aaaaggagat ggctgaaaga gccaaggaga gcattgacag cctcgagagc gccaacccca
     6301 agctgtatgc caattacatc aaatacagcg acgagaagaa agccgaggag ttcaccaggc
     6361 agattaacag ggagaaggcc aaaaccgccc tgaacgccta cctgaggaac accaagtgga
     6421 atgtgatcat cagggaggac ctcctgagaa ttgacaacaa gacatgtacc ctgttcagaa
     6481 acaaggccgt ccacctggaa gtggccaggt atgtccacgc ctatatcaac gacattgccg
     6541 aggtcaattc ctacttccaa ctgtaccatt acatcatgca gagaattatc atgaatgaga
     6601 ggtacgagaa aagcagcgga aaggtgtccg agtacttcga cgctgtgaat gacgagaaga
     6661 agtacaacga taggctcctg aaactgctgt gtgtgccttt cggctactgt atccccaggt
     6721 ttaagaacct gagcatcgag gccctgttcg ataggaacga ggccgccaag ttcgacaagg
     6781 agaaaaagaa ggtgtccggc aattccggat ccggacctaa gaaaaagagg aaggtggcgg
     6841 ccgcttaccc atacgatgtt ccagattacg ctgctagcgg cagtggagag ggcagaggaa
     6901 gtctgctaac atgcggtgac gtcgaggaga atcctggccc agtgagcaag ggcgaggagc
     6961 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     7021 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7081 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     7141 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     7201 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     7261 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     7321 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     7381 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     7441 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     7501 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     7561 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     7621 ccgggatcac tctcggcatg gacgagctgt acaagtaaga attcgatatc aagcttatcg
     7681 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7741 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7801 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7861 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7921 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7981 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8041 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8101 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8161 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8221 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     8281 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     8341 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     8401 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8461 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     8521 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     8581 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     8641 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     8701 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     8761 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     8821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8941 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     9001 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     9061 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     9121 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     9181 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     9241 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     9301 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9361 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9421 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9481 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9541 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9601 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9661 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9721 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9781 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9841 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9961 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
    10021 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
    10081 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
    10141 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
    10201 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
    10261 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
    10321 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
    10381 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
    10441 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
    10501 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
    10561 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    10621 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    10681 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10741 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10801 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    11521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    11581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    11641 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    11701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    11761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    12121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    12181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    12241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    12301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    12361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    12421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    12481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    12541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    12601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    12661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    12721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12961 gcgcacattt ccccgaaaag tgccacctga c
//
