LOCUS       40924_47243        6000 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXNS2pat-YFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Dahncke K, Witte CP.
  TITLE     Plant purine nucleoside catabolism employs a guanosine deaminase 
            required for the generation of xanthosine in Arabidopsis
  JOURNAL   Plant Cell 25 (10), 4101-4109 (2013)
  PUBMED    24130159
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Dahncke K, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2013) Department of Plant Biochemistry, Freie 
            Universitaet Berlin, Dahlem Centre of Plant Sciences, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 6000)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Cell"; date: "2013"; volume: "25"; issue: "10"; pages: "4101-4109"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-AUG-2013) Department of Plant Biochemistry, Freie Universitaet 
            Berlin, Dahlem Centre of Plant Sciences, Koenigin-Luise-Str. 12-16, 
            Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             30..746
                     /codon_start=1
                     /label=EYFP
                     /note="enhanced YFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(778..794)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1077..1101
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      1213..1922
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1996..2087
                     /label=AmpR promoter
     CDS             2088..2945
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3119..3707
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4019..4043
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4137..4389)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4412..4960)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5003..5182)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5326..5999
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 tcgagctctt caaacaaata acaccaaaaa tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcgtgcagt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      481 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagggcg cctccatgga attcctgcag cccgggggat
      781 ccactagttc tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta
      841 tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg
      901 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
      961 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgaccgggc ggccgccacc
     1021 gcggtggagg gggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
     1081 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     1141 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgtacatca ccgacgagca
     1201 aggcaagacc gagcgccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg
     1261 cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc
     1321 ggccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg
     1381 gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct
     1441 cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt
     1501 tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga
     1561 cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag
     1621 agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa
     1681 tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct
     1741 gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga
     1801 ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg
     1861 cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc
     1921 agcgctggca gtccttgcca ttgccgggat cggggcagta acgggatggg cgatcagccc
     1981 gacaagctac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2041 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2101 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2161 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2221 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2281 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2341 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2401 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2461 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2521 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2581 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2641 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2701 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2761 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2821 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3241 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggac tctagctaga
     3781 ggatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     3841 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     3901 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     3961 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     4021 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     4081 cgtttttaat gatcgaatac taacgtctct accagatatc agcttgcatg ccggtcgatc
     4141 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     4201 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     4261 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     4321 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     4381 tgaacgatct gcttgactct aggggtcatc agatttcggt gacgggcagg accggacggg
     4441 gcggcaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct
     4501 tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca
     4561 cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca
     4621 gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg
     4681 agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg
     4741 cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc
     4801 gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga
     4861 ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg
     4921 tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat ggtagatccc cctcgatcga
     4981 gttgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg
     5041 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     5101 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt
     5161 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     5221 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcagatt gtcgtttccc
     5281 gccttcggtt tgggcgcgcc cggtctcaga agaccagagg gctattgaga cttttcaaca
     5341 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
     5401 aaggacagta gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc
     5461 tatcgttcaa gaatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga
     5521 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     5581 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     5641 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     5701 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
     5761 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac cgacagtggt
     5821 cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg
     5881 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     5941 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggacc
//
