LOCUS       40924_47238        5316 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXNS2pat-Strep, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Cao FQ, Werner AK, Dahncke K, Romeis T, Liu LH, Witte CP.
  TITLE     Identification and characterization of proteins involved in rice 
            urea and arginine catabolism
  JOURNAL   Plant Physiol. 154 (1), 98-108 (2010)
  PUBMED    20631318
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Cao FQ, Dahncke K, Liu LH, Romeis T, Werner AK, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2010) Dahlem Centre of Plant Science, Department 
            of Plant Biochemistry, Freie Universitaet Berlin, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5316)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5316)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2010"; volume: "154"; issue: "1"; pages: "98-108"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2010) Dahlem Centre of Plant Science, Department of Plant 
            Biochemistry, Freie Universitaet Berlin, Koenigin-Luise-Str. 12-16, 
            Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             39..62
                     /codon_start=1
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
                     /translation="WSHPQFEK"
     primer_bind     complement(94..110)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    393..417
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      529..1238
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1312..1403
                     /label=AmpR promoter
     CDS             1404..2261
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2435..3023
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3335..3359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3453..3705)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3728..4276)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4319..4498)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4642..5315
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 tcgagctctt caaacaaata acaccaaaaa tggcatcttg gtctcatcct caatttgaaa
       61 aaggcgcctc catggaattc ctgcagcccg ggggatccac tagttctaga gtccgcaaaa
      121 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
      181 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
      241 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
      301 ctaaaaccaa aatccagtga ccgggcggcc gccaccgcgg tggaggggga tcagattgtc
      361 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
      421 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
      481 gttcgtccat ttgtatgtgt acatcaccga cgagcaaggc aagaccgagc gcctttccga
      541 cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg
      601 cgccagaaac gccgtcgaag ccgtgtgcga gacaccggcc gccggcgttg tggatacctc
      661 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
      721 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
      781 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
      841 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
      901 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
      961 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
     1021 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
     1081 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
     1141 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
     1201 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg ctggcagtcc ttgccattgc
     1261 cgggatcggg gcagtaacgg gatgggcgat cagcccgaca agctacccct atttgtttat
     1321 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1381 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1441 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1501 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1561 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1621 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1681 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1741 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1801 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1861 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1921 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1981 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2041 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2101 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2161 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2221 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2281 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2341 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2401 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2461 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2521 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2581 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2641 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2701 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2761 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2821 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2881 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2941 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3001 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3061 tttgctggcc ttttgctcac atggactcta gctagaggat cacaggcagc aacgctctgt
     3121 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     3181 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     3241 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     3301 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     3361 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgatc gaatactaac
     3421 gtctctacca gatatcagct tgcatgccgg tcgatctagt aacatagatg acaccgcgcg
     3481 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
     3541 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
     3601 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
     3661 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg
     3721 gtcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca
     3781 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc
     3841 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga
     3901 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga
     3961 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg
     4021 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc
     4081 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc
     4141 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt
     4201 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc
     4261 gtcgttctgg gctcatggta gatccccctc gatcgagttg agagtgaata tgagactcta
     4321 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     4381 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     4441 cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg
     4501 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     4561 cttaattctc cgctcatgat cagattgtcg tttcccgcct tcggtttggg cgcgcccggt
     4621 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     4681 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
     4741 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagaat gcctctaccg
     4801 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
     4861 caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacactctcg
     4921 tctactccaa gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc
     4981 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     5041 tcgaaaggac agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa
     5101 aggctatcgt tcaagaatgc ctctaccgac agtggtccca aagatggacc cccacccacg
     5161 aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     5221 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     5281 tctatataag gaagttcatt tcatttggag aggacc
//
