LOCUS       62056_22840        8735 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2169..2186
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2403..2489
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2581..2608
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2627..2718
                     /label=AmpR promoter
     rep_origin      complement(3105..3693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6547..7203)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(8355..8432)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        8662..8690
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    8698..8714
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattaagctt aaaggaggac aaccatgcca ctgttcggat ccttctccga ccgcgtcaac
       61 cgtgcagtgc tctacaggat ctgtgcaaag gaggacaact aatgcaaatc aaccgccgcg
      121 gctttttaaa ggcaactgct ggtttagcaa ccatcggcgc agcatccatg ttcatgccaa
      181 aggccaacgc ccttggagca ctcccggcga accaccgatc ggggtcgatc gaggacgtcg
      241 agcacatcgt cgtcctgatg caggagaacc gttccttcga ccactacttc ggcacactgc
      301 gcggggtccg cggcttcggc gacccgcatc cggtgcgact ggacgacggc aggtcggtgt
      361 ggcaccagcg caagggcgac ggcaccgagg tactaccctt ccacccggag gccgacgacc
      421 tcggtatgca gttcctcgaa gggctcccgc acggctggtc cgacggccag gacgcctacc
      481 acgacgccaa gtacgaccgc tggctgcccg ccaagggcac caccaccatg gcgtacctga
      541 cccgcgagga catccccttc cactacgcgc tcgccgacac cttcaccgtc tgcgacgcct
      601 accactgctc cttcatcggc tccaccgacc ccaaccgcta ctacctgtgg tcggggcaca
      661 cgggcaacga cggcgcgggc ggcggcccgg tcctcggcaa cgacgaactc ggctacgact
      721 ggacgacgta ccccgagcgc ctggaggcgg ccggggtctc ctggaagatc taccaggacg
      781 tcggcgacgg cctcgacgcg gccggccact ggggctggat cgacgacgcc taccggggca
      841 actacggcga caactccctg ctgtacttca acaagtaccg gaacgcgaag cccggcgacc
      901 cgctgtacga caaggcccgc accggcaccg acgcgaagcg cggcgagggc tacttcgacc
      961 ggctccgtgc cgacgtcaag gcgggcaagc tgccggagat ctcctggata gccgccccgg
     1021 aggcattctc cgagcactcc aactggccgt cgaactacgg cgcctggtac atctcccagg
     1081 tcctggacgc gctcacctcc aacccgaagg tgtgggcgaa gaccgccctg ttcatcacgt
     1141 acgacgagaa cgacggcttc ttcgaccacg tggtgccgcc gctgccgccg aagtccgccg
     1201 cgcagggccg gtccaccgtc gacgtctccc tggacgtctt cgagggcagc gccaagcacc
     1261 gcgagggctt ctacgggctc ggcccgcgcg tgcccatgct ggtcgtctcg ccgtggagca
     1321 agggcggctt cgtctgctcc gagaccttcg accacacctc ggtcatccgc ttcatggaac
     1381 gccgcttcgg cgtccacgag ccgaacatct caccctggcg gcgcgccgtc tgcggcgacc
     1441 tgacctccgc cttcgacttc tcccgcacgg acagccgccc ggcccggctg ccggacaccg
     1501 acgcctacga gccgccggac cgcgaacgcc accccgacta ccgtccgacg ccgcccgcgg
     1561 accccggcat gccccggcag gaacgcggga cacgccccac ccggccgctg cgctacgccc
     1621 cgcacgtcga cggcgccgtg gacacggcgg ccgggaagtt cacgctggcc ttcgcctccg
     1681 ggccccgggc cggtgccgcc ttcctcgtca cctccggcaa ccgcaccgac ggcccctgga
     1741 cgtacaccac cgaggccggc aaggacatcg cggacacctg gaactcggcc tactccggcg
     1801 gctcctacga cctgaccgtg cacggcccgg ccggcttcct gcgggtcttc cggggtgcca
     1861 acgaggtcgc cggacccgcc gggcccgagg tcaccgcacg gcaccgcggc gacgacctcc
     1921 ggctgaccct cacccacacc gggaccggca ccgtccggct ccggatcgcg aacgcccacg
     1981 acggccgcac ccggtccgtc accgtccgcc ccggcgccac cgtgcaccac accgtcggcc
     2041 tcgcgcgcag ccggcgctgg tacgacgtga cggtcacctc cgagaccgcc ccggccttcc
     2101 tgcgccgctt cgccgggcac gtggagaacg gccggccggg gaccagcgac ccggcgatcg
     2161 tcacggacca tcatcaccat caccattgag aattcagctt ggctgttttg gcggatgaga
     2221 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
     2281 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     2341 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
     2401 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2461 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     2521 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
     2581 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
     2641 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2701 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2761 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2821 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2881 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttgc ttcctcgctc
     2941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     3301 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3541 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3781 aggatcttca cctagatcct tttggggtgg gcgaagaact ccagcatgag atccccgcgc
     3841 tggaggatca tccagccatt cggggtcgtt cactggttcc cctttctgat ttctggcata
     3901 gaagaacccc cgtgaactgt gtggttccgg gggttgctga tttttgcgag acttctcgcg
     3961 caattcccta gcttaggtga aaacaccatg aaacactagg gaaacaccca tgaaacaccc
     4021 attagggcag tagggcggct tcttcgtcta gggcttgcat ttgggcggtg atctggtctt
     4081 tagcgtgtga aagtgtgtcg taggtggcgt gctcaatgca ctcgaacgtc acgtcattta
     4141 ccgggtcacg gtgggcaaag agaactagtg ggttagacat tgttttcctc gttgtcggtg
     4201 gtggtgagct tttctagccg ctcggtaaac gcggcgatca tgaactcttg gaggttttca
     4261 ccgttctgca tgcctgcgcg cttcatgtcc tcacgtagtg ccaaaggaac gcgtgcggtg
     4321 accacgacgg gcttagcctt tgcctgcgct tctagtgctt cgatggtggc ttgtgcctgc
     4381 gcttgctgcg cctgtagtgc ctgttgagct tcttgtagtt gctgttctag ctgtgccttg
     4441 gttgccatgc tttaagactc tagtagcttt cctgcgatat gtcatgcgca tgcgtagcaa
     4501 acattgtcct gcaactcatt cattatgtgc agtgctcctg ttactagtcg tacatactca
     4561 tatttaccta gtctgcatgc agtgcatgca catgcagtca tgtcgtgcta atgtgtaaaa
     4621 catgtacatg cagattgctg ggggtgcagg gggcggagcc accctgtcca tgcggggtgt
     4681 ggggcttgcc ccgccggtac agacagtgag caccggggca cctagtcgcg gatacccccc
     4741 ctaggtatcg gacacgtaac cctcccatgt cgatgcaaat ctttaacatt gagtacgggt
     4801 aagctggcac gcatagccaa gctaggcggc caccaaacac cactaaaaat taatagtccc
     4861 tagacaagac aaacccccgt gcgagctacc aactcatatg cacgggggcc acataacccg
     4921 aaggggtttc aattgacaac catagcacta gctaagacaa cgggcacaac acccgcacaa
     4981 actcgcactg cgcaaccccg cacaacatcg ggtctaggta acactgagta acactgaaat
     5041 agaagtgaac acctctaagg aaccgcaggt caatgagggt tctaaggtca ctcgcgctag
     5101 ggcgtggcgt aggcaaaacg tcatgtacaa gatcaccaat agtaaggctc tggcggggtg
     5161 ccataggtgg cgcagggacg aagctgttgc ggtgtcctgg tcgtctaacg gtgcttcgca
     5221 gtttgagggt ctgcaaaact ctcactctcg ctgggggtca cctctggctg aattggaagt
     5281 catgggcgaa cgccgcattg agctggctat tgctactaag aatcacttgg cggcgggtgg
     5341 cgcgctcatg atgtttgtgg gcactgttcg acacaaccgc tcacagtcat ttgcgcaggt
     5401 tgaagcgggt attaagactg cgtactcttc gatggtgaaa acatctcagt ggaagaaaga
     5461 acgtgcacgg tacggggtgg agcacaccta tagtgactat gaggtcacag actcttgggc
     5521 gaacggttgg cacttgcacc gcaacatgct gttgttcttg gatcgtccac tgtctgacga
     5581 tgaactcaag gcgtttgagg attccatgtt ttcccgctgg tctgctggtg tggttaaggc
     5641 cggtatggac gcgccactgc gtgagcacgg ggtcaaactt gatcaggtgt ctacctgggg
     5701 tggagacgct gcgaaaatgg caacctacct cgctaagggc atgtctcagg aactgactgg
     5761 ctccgctact aaaaccgcgt ctaaggggtc gtacacgccg tttcagatgt tggatatgtt
     5821 ggccgatcaa agcgacgccg gcgaggatat ggacgctgtt ttggtggctc ggtggcgtga
     5881 gtatgaggtt ggttctaaaa acctgcgttc gtcctggtca cgtggggcta agcgtgcttt
     5941 gggcattgat tacatagacg ctgatgtacg tcgtgaaatg gaagaagaac tgtacaagct
     6001 cgccggtctg gaagcaccgg aacgggtcga atcaacccgc gttgctgttg ctttggtgaa
     6061 gcccgatgat tggaaactga ttcagtctga tttcgcggtt aggcagtacg ttctcgattg
     6121 cgtggataag gctaaggacg tggccgctgc gcaacgtgtc gctaatgagg tgctggcaag
     6181 tctgggtgtg gattccaccc cgtgcatgat cgttatggat gatgtggact tggacgcggt
     6241 tctgcctact catggggacg ctactaagcg tgatctgaat gcggcggtgt tcgcgggtaa
     6301 tgagcagact attcttcgca cccactaaaa gcggcataaa ccccgttcga tattttgtgc
     6361 gatgaattta tggtcaatgt cgcgggggca aactatgatg ggtcttgttg ttggcgtccc
     6421 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg
     6481 atggcaggtt gggcgtcgct tggtcggtca tttcgaaggg caccaataac tgccttaaaa
     6541 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     6601 gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt
     6661 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     6721 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     6781 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     6841 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     6901 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     6961 ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat
     7021 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     7081 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     7141 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     7201 cattttagct tccttagctc ctgaaaatct cgtcgaagct cggcggattt gtcctactca
     7261 agctgatccg acaaaatcca cacattatcc caggtgtccg gatcggtcaa atacgctgcc
     7321 agctcataga ccgtatccaa agcatccggg gctgatcccc ggcgccaggg tggtttttct
     7381 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     7441 cagcaagcgg tccacgtggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     7501 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     7561 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     7621 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     7681 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     7741 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     7801 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     7861 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     7921 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     7981 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     8041 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     8101 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     8161 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     8221 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     8281 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     8341 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     8401 ccgcgaaagg ttttgcacca ttcgatggtg tcaacgtaaa tgccgcttcg ccttcgcgcg
     8461 cgaattgcaa gctgatccgg gcttatcgac tgcacggtgc accaatgctt ctggcgtcag
     8521 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
     8581 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
     8641 caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg
     8701 tgagcggata acaatttcac acaggaaaca gaatt
//
