LOCUS       62056_22830        8729 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2163..2180
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2397..2483
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2575..2602
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2621..2712
                     /label=AmpR promoter
     rep_origin      complement(3099..3687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6541..7197)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(8349..8426)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        8656..8684
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    8692..8708
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattaagctt aaaggaggac aaccatgcca ctgttcggat ccttctccga ccgcgtcaac
       61 cgtgcagtgc tctacaggat ctgtgcaaag gaggacaact aatggccgaa gtgaaccggc
      121 gcagattcct ccaactggcg ggcgccacca cggcgttcag tgccctgtcc gcgagcatcg
      181 accgggccgc ggcactcccg gcgaaccacc gatcggggtc gatcgaggac gtcgagcaca
      241 tcgtcgtcct gatgcaggag aaccgttcct tcgaccacta cttcggcaca ctgcgcgggg
      301 tccgcggctt cggcgacccg catccggtgc gactggacga cggcaggtcg gtgtggcacc
      361 agcgcaaggg cgacggcacc gaggtactac ccttccaccc ggaggccgac gacctcggta
      421 tgcagttcct cgaagggctc ccgcacggct ggtccgacgg ccaggacgcc taccacgacg
      481 ccaagtacga ccgctggctg cccgccaagg gcaccaccac catggcgtac ctgacccgcg
      541 aggacatccc cttccactac gcgctcgccg acaccttcac cgtctgcgac gcctaccact
      601 gctccttcat cggctccacc gaccccaacc gctactacct gtggtcgggg cacacgggca
      661 acgacggcgc gggcggcggc ccggtcctcg gcaacgacga actcggctac gactggacga
      721 cgtaccccga gcgcctggag gcggccgggg tctcctggaa gatctaccag gacgtcggcg
      781 acggcctcga cgcggccggc cactggggct ggatcgacga cgcctaccgg ggcaactacg
      841 gcgacaactc cctgctgtac ttcaacaagt accggaacgc gaagcccggc gacccgctgt
      901 acgacaaggc ccgcaccggc accgacgcga agcgcggcga gggctacttc gaccggctcc
      961 gtgccgacgt caaggcgggc aagctgccgg agatctcctg gatagccgcc ccggaggcat
     1021 tctccgagca ctccaactgg ccgtcgaact acggcgcctg gtacatctcc caggtcctgg
     1081 acgcgctcac ctccaacccg aaggtgtggg cgaagaccgc cctgttcatc acgtacgacg
     1141 agaacgacgg cttcttcgac cacgtggtgc cgccgctgcc gccgaagtcc gccgcgcagg
     1201 gccggtccac cgtcgacgtc tccctggacg tcttcgaggg cagcgccaag caccgcgagg
     1261 gcttctacgg gctcggcccg cgcgtgccca tgctggtcgt ctcgccgtgg agcaagggcg
     1321 gcttcgtctg ctccgagacc ttcgaccaca cctcggtcat ccgcttcatg gaacgccgct
     1381 tcggcgtcca cgagccgaac atctcaccct ggcggcgcgc cgtctgcggc gacctgacct
     1441 ccgccttcga cttctcccgc acggacagcc gcccggcccg gctgccggac accgacgcct
     1501 acgagccgcc ggaccgcgaa cgccaccccg actaccgtcc gacgccgccc gcggaccccg
     1561 gcatgccccg gcaggaacgc gggacacgcc ccacccggcc gctgcgctac gccccgcacg
     1621 tcgacggcgc cgtggacacg gcggccggga agttcacgct ggccttcgcc tccgggcccc
     1681 gggccggtgc cgccttcctc gtcacctccg gcaaccgcac cgacggcccc tggacgtaca
     1741 ccaccgaggc cggcaaggac atcgcggaca cctggaactc ggcctactcc ggcggctcct
     1801 acgacctgac cgtgcacggc ccggccggct tcctgcgggt cttccggggt gccaacgagg
     1861 tcgccggacc cgccgggccc gaggtcaccg cacggcaccg cggcgacgac ctccggctga
     1921 ccctcaccca caccgggacc ggcaccgtcc ggctccggat cgcgaacgcc cacgacggcc
     1981 gcacccggtc cgtcaccgtc cgccccggcg ccaccgtgca ccacaccgtc ggcctcgcgc
     2041 gcagccggcg ctggtacgac gtgacggtca cctccgagac cgccccggcc ttcctgcgcc
     2101 gcttcgccgg gcacgtggag aacggccggc cggggaccag cgacccggcg atcgtcacgg
     2161 accatcatca ccatcaccat tgagaattca gcttggctgt tttggcggat gagagaagat
     2221 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
     2281 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     2341 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     2401 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2461 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     2521 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     2581 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
     2641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttgcttcctc gctcactgac
     2941 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3001 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3061 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     3301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3781 ttcacctaga tccttttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     3841 atcatccagc cattcggggt cgttcactgg ttcccctttc tgatttctgg catagaagaa
     3901 cccccgtgaa ctgtgtggtt ccgggggttg ctgatttttg cgagacttct cgcgcaattc
     3961 cctagcttag gtgaaaacac catgaaacac tagggaaaca cccatgaaac acccattagg
     4021 gcagtagggc ggcttcttcg tctagggctt gcatttgggc ggtgatctgg tctttagcgt
     4081 gtgaaagtgt gtcgtaggtg gcgtgctcaa tgcactcgaa cgtcacgtca tttaccgggt
     4141 cacggtgggc aaagagaact agtgggttag acattgtttt cctcgttgtc ggtggtggtg
     4201 agcttttcta gccgctcggt aaacgcggcg atcatgaact cttggaggtt ttcaccgttc
     4261 tgcatgcctg cgcgcttcat gtcctcacgt agtgccaaag gaacgcgtgc ggtgaccacg
     4321 acgggcttag cctttgcctg cgcttctagt gcttcgatgg tggcttgtgc ctgcgcttgc
     4381 tgcgcctgta gtgcctgttg agcttcttgt agttgctgtt ctagctgtgc cttggttgcc
     4441 atgctttaag actctagtag ctttcctgcg atatgtcatg cgcatgcgta gcaaacattg
     4501 tcctgcaact cattcattat gtgcagtgct cctgttacta gtcgtacata ctcatattta
     4561 cctagtctgc atgcagtgca tgcacatgca gtcatgtcgt gctaatgtgt aaaacatgta
     4621 catgcagatt gctgggggtg cagggggcgg agccaccctg tccatgcggg gtgtggggct
     4681 tgccccgccg gtacagacag tgagcaccgg ggcacctagt cgcggatacc ccccctaggt
     4741 atcggacacg taaccctccc atgtcgatgc aaatctttaa cattgagtac gggtaagctg
     4801 gcacgcatag ccaagctagg cggccaccaa acaccactaa aaattaatag tccctagaca
     4861 agacaaaccc ccgtgcgagc taccaactca tatgcacggg ggccacataa cccgaagggg
     4921 tttcaattga caaccatagc actagctaag acaacgggca caacacccgc acaaactcgc
     4981 actgcgcaac cccgcacaac atcgggtcta ggtaacactg agtaacactg aaatagaagt
     5041 gaacacctct aaggaaccgc aggtcaatga gggttctaag gtcactcgcg ctagggcgtg
     5101 gcgtaggcaa aacgtcatgt acaagatcac caatagtaag gctctggcgg ggtgccatag
     5161 gtggcgcagg gacgaagctg ttgcggtgtc ctggtcgtct aacggtgctt cgcagtttga
     5221 gggtctgcaa aactctcact ctcgctgggg gtcacctctg gctgaattgg aagtcatggg
     5281 cgaacgccgc attgagctgg ctattgctac taagaatcac ttggcggcgg gtggcgcgct
     5341 catgatgttt gtgggcactg ttcgacacaa ccgctcacag tcatttgcgc aggttgaagc
     5401 gggtattaag actgcgtact cttcgatggt gaaaacatct cagtggaaga aagaacgtgc
     5461 acggtacggg gtggagcaca cctatagtga ctatgaggtc acagactctt gggcgaacgg
     5521 ttggcacttg caccgcaaca tgctgttgtt cttggatcgt ccactgtctg acgatgaact
     5581 caaggcgttt gaggattcca tgttttcccg ctggtctgct ggtgtggtta aggccggtat
     5641 ggacgcgcca ctgcgtgagc acggggtcaa acttgatcag gtgtctacct ggggtggaga
     5701 cgctgcgaaa atggcaacct acctcgctaa gggcatgtct caggaactga ctggctccgc
     5761 tactaaaacc gcgtctaagg ggtcgtacac gccgtttcag atgttggata tgttggccga
     5821 tcaaagcgac gccggcgagg atatggacgc tgttttggtg gctcggtggc gtgagtatga
     5881 ggttggttct aaaaacctgc gttcgtcctg gtcacgtggg gctaagcgtg ctttgggcat
     5941 tgattacata gacgctgatg tacgtcgtga aatggaagaa gaactgtaca agctcgccgg
     6001 tctggaagca ccggaacggg tcgaatcaac ccgcgttgct gttgctttgg tgaagcccga
     6061 tgattggaaa ctgattcagt ctgatttcgc ggttaggcag tacgttctcg attgcgtgga
     6121 taaggctaag gacgtggccg ctgcgcaacg tgtcgctaat gaggtgctgg caagtctggg
     6181 tgtggattcc accccgtgca tgatcgttat ggatgatgtg gacttggacg cggttctgcc
     6241 tactcatggg gacgctacta agcgtgatct gaatgcggcg gtgttcgcgg gtaatgagca
     6301 gactattctt cgcacccact aaaagcggca taaaccccgt tcgatatttt gtgcgatgaa
     6361 tttatggtca atgtcgcggg ggcaaactat gatgggtctt gttgttggcg tcccggaaaa
     6421 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     6481 ggttgggcgt cgcttggtcg gtcatttcga agggcaccaa taactgcctt aaaaaaatta
     6541 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     6601 gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     6661 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     6721 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     6781 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     6841 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     6901 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     6961 gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa gaatgtgaat
     7021 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     7081 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     7141 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     7201 agcttcctta gctcctgaaa atctcgtcga agctcggcgg atttgtccta ctcaagctga
     7261 tccgacaaaa tccacacatt atcccaggtg tccggatcgg tcaaatacgc tgccagctca
     7321 tagaccgtat ccaaagcatc cggggctgat ccccggcgcc agggtggttt ttcttttcac
     7381 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     7441 gcggtccacg tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     7501 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
     7561 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     7621 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     7681 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     7741 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     7801 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     7861 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     7921 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     7981 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     8041 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     8101 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     8161 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     8221 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     8281 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     8341 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     8401 aaggttttgc accattcgat ggtgtcaacg taaatgccgc ttcgccttcg cgcgcgaatt
     8461 gcaagctgat ccgggcttat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc
     8521 atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa
     8581 ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata
     8641 ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
     8701 gataacaatt tcacacagga aacagaatt
//
