LOCUS       V002033                 5611 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002033
VERSION     V002033
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Mokrejs M, Vopalensky V, Kolenaty O, Masek T, Feketova Z, Sekyrova
            P, Skaloudova B, Kriz V, Pospisek M.
  TITLE     IRESite: the database of experimentally verified IRES structures
            (www.iresite.org)
  JOURNAL   Nucleic Acids Res. 34 (DATABASE ISSUE), D125-D130 (2006)
   PUBMED   16381829
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Masek T, Mokrejs M, Vopalensky V, Pospisek M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2008) Genetics and Microbiology, Faculty of
            Science, Charles University in Prague, Vinicna 5, Prague 128 44,
            Czech Republic
REFERENCE   3  (bases 1 to 5611)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. 34 (DATABASE ISSUE), D125-D130 (2006)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-JUL-2008) Genetics and Microbiology, Faculty of Science, Charles
            University in Prague, Vinicna 5, Prague 128 44, Czech Republic"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        807..911
                     /label="AmpR promoter"
     CDS             912..1769
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2783..2801
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2838..4013
                     /gene="ccnb2"
                     /label="G2/mitotic-specific cyclin-B2"
                     /note="G2/mitotic-specific cyclin-B2 from Xenopus laevis.
                     Accession#: P13351"
     CDS             4753..5421
                     /gene="NS"
                     /label="Non-structural protein 1"
                     /note="Non-structural protein 1 from Influenza A virus
                     (strain A/Russia:St.Petersburg/8/2006 H1N1). Accession#:
                     B4URE2"
     promoter        complement(join(5610..5611,1..17))
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
      121 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
      181 ctggtaacgc cgttccgcac cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
      241 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      421 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      481 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgatatgg tgcactctca
      541 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      601 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      661 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      721 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      961 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1021 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1081 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1141 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1201 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1261 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1321 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1381 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1441 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1501 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1561 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1621 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1681 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1741 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1801 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1861 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1921 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1981 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2041 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2101 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2161 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2281 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2341 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2401 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2461 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2521 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2581 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2641 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2701 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2761 aatgcagctg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgggcaa
     2821 acgttatttt accggagatg gctactcgtc gcgctgctat tccccgtgaa gcggataata
     2881 tccttggggg tgcaatgcga tccaaagttc aaatgaatag cagacgagct gctttgggag
     2941 agattggcaa caaagtgact gtgcgaggaa aaccacctgc agtaaagcag tcttccaatg
     3001 ctgtggcaaa gccttcaaag atggcagcaa ctaaagtggc aaatgttaag actaagcatg
     3061 tacctgtgaa accagttgta gctgaagctg cccccaaagt gccttcccct gtgccgatgg
     3121 atgtgtcctt gaaagaggaa gagctgtgcc aggcattctc cgatgcactg accagtgttg
     3181 aagacattga tgcagatgat ggtggaaacc ctcaattgtg cagtgactat gtgatggaca
     3241 tctataacta ccttaagcaa ctggaagttc aacagtctgt acatccttgc tatcttgaag
     3301 gaaaagaaat taatgagcgt atgagagcta tcctagttga ctggcttgtt caagtgcatt
     3361 ctaggtttca gcttcttcag gagactttat acatgggcgt tgcaatcatg gatcgcttct
     3421 tacaagttca gccagtctcc cgcagtaagc ttcagttggt tggtgttact tccctactaa
     3481 ttgcttcaaa atatgaagag atgtacactc cagaggttgc agactttgtt tatatcaccg
     3541 ataatgccta tactgcatct cagatcagag aaatggaaat gattatcctt cgactcctca
     3601 actttgacct tggacggcca ttacctctcc acttcctcag acgggcttca aaatcttgca
     3661 gtgctgatgc agagcaacat acccttgcaa aatatttgat ggagcttaca ctcatagact
     3721 atgaaatggt ccacatcaag ccttcagaaa ttgcagctgc tgccctctgc ctatctcaaa
     3781 aaattcttgg ccagggaacc tggggtacca ctcagcacta ttacacaggc tacacagaag
     3841 gtgacttgca actgatcatg aagcatatgg ctaagaacat aaccaaagtc aaccagaatc
     3901 taacaaagca tgtggctgtg aggaacaagt atgctagcag taaactgatg aagatcagca
     3961 cccttcctca gcttatggct cctctaatca cagagcttgc tgcaagtctc tcttagaact
     4021 gttaagtgac cctttcaaag agaaccacta attgcacttt taacgttgct gctggcaaat
     4081 gctgctgttc ctgtaaatat gtttgtattt ttattgactc atttgtgaac tttgagtaat
     4141 caatgctgta atgtcagtat gaaataaaac ttcacatttt ttatttggtc gacgagctcg
     4201 gtaccatgga tcaattcggt tcggtgttct tctcaaataa aaagattgta atcatttagt
     4261 gaaataaaaa ttatattttt ttcacttata aatattgcaa gtatttaatt ggcgcagtcg
     4321 gtaggatcca ataaaataaa agagtccttt tagtacggta ctgatcaact gaaggatatg
     4381 ctatacgact agctatccaa gatcagcgaa ttaaaatagt gattcaaaaa tattttttaa
     4441 tccgcaaaag aatctacgtg aaagtagtat tcaaaataaa atcccgtgcg gtcggaaaca
     4501 aaaattaatt taaatttttt aattccgaaa cttaaaacca agtttaaaga aaacttaaaa
     4561 tcaagaaaac ttaaaaccaa gtttaaagaa aacttaaaat caagaaaact aaagaccaag
     4621 tttaaagaaa acttaagatc aagaaaactt aaaaccaagt ttaaagaaaa ctcaaaatca
     4681 agaaaactta aaaccaaaat aagctccaga aaactaaaag acatcatggc agtcccacaa
     4741 cgaattgatc ccagctttca ggtagattgc tttctttggc atgtccgcaa acgagttgca
     4801 gaccaagaac taggtgatgc cccattcctt gatcggcttc gccgagatca gaaatcccta
     4861 agaggaaggg gcagcaccct cggtctggac atcgagacag ccacacgtgc tggaaagcag
     4921 atagtggagc ggattctgaa agaagaatcc gatgaggcac ttaaaatgac catggcctct
     4981 gtacctgcgt cgcgttacct aactgacatg actcttgagg aaatgtcaag ggactggtcc
     5041 atgctcatac ccaagcagaa agtggcaggc cctctttgta tcagaatgga ccaggcgatc
     5101 atggataaga acatcatact gaaagcgaac ttcagtgtga tttttgaccg gctggagact
     5161 ctaatattgc taagggcttt caccgaagag ggagcaattg ttggcgaaat ttcaccattg
     5221 ccttctcttc caggacatac tgctgaggat gtcaaaaatg cagttggagt cctcatcggg
     5281 ggacttgaat ggaatgataa cacagttcga gtctctgaaa ctctacagag attcgcttgg
     5341 agaagcagta atgagaatgg gagacctcca ctcactccaa aacagaaacg agaaatggcg
     5401 ggaacaatta ggtcagaagt ttgaagaaat aagatggttg attgaagaag tgagacacaa
     5461 actgaagata acagagaata gttttgagca aataacattt atgcaagcct tacatctatt
     5521 gcttgaagtg gagcaagaga taagaacttt ctcgtttcag cttatttaat aataaaaaac
     5581 acccttgttt ctactggaat tccgtgtatt c
//
