LOCUS       62056_22825        4339 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(709..830)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(901..924)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        complement(939..957)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          complement(1012..1584)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(1746..1949)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1950..2253)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      2332..2787
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3119..3223
                     /label=AmpR promoter
     CDS             3224..4081
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4255..4339
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
       61 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      121 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      181 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      241 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      301 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      361 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      421 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      481 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      541 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      601 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      661 gagtcagtga gcgaggaagc ggaagagtct agagtcgacc agacatgata agatacattg
      721 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      781 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ctgctttaat
      841 aagatctggt accgagctcc tgcagcccgg ggcggccgcc tcgagaagct tggatccgga
      901 cttatcatcg tcgtccttgt agtccatggt gaattcgccc tatagtgagt cgtattacaa
      961 ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa
     1021 aaagaagaag gcatgaacat ggttagcaga ggggcccggt ttggactcag agtattttat
     1081 cctcatctca aacagtgtat atcattgtaa ccataaagag aaaggcagga tgatgaccag
     1141 gatgtagttg tttctaccaa taagaatatt tccacgccag ccagaattta tatgcagaaa
     1201 tattctacct tatcatttaa ttataacaat tgttctctaa aactgtgctg aagtacaata
     1261 taatataccc tgattgcctt gaaaaaaaag tgattagaga aagtacttac aatctgacaa
     1321 ataaacaaaa gtgaatttaa aaattcgtta caaatgcaag ctaaagttta acgaaaaagt
     1381 tacagaaaat gaaaagaaaa taagaggaga caatggttgt caacagagta gaaagtgaaa
     1441 gaaacaaaat tatcatgagg gtccatggtg atacaaggga catcttccca ttctaaacaa
     1501 caccctgaaa actttgcccc ctccatataa catgaatttt acaatagcga aaaagaaaga
     1561 acaatcaagg gtccccaaac tcaccctgaa gttctcagga tcgatccgga gctttttgca
     1621 aaagcctagg ggtccgttcc aatgcaccgt tcccggccgc ggaggctgga tcggtcccgg
     1681 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
     1741 aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     1801 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     1861 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     1921 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     1981 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     2041 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     2101 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     2161 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     2221 ccaggcgggc catttaccgt aagttatgta acggactcta gctggcgtaa tagcgaagag
     2281 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     2341 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2401 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2461 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2521 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     2581 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2641 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2701 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2761 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     2821 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2881 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2941 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3001 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3061 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4321 ggtttgtttg ccggatcaa
//
