LOCUS       62056_22820        4342 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(709..830)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(901..927)
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        complement(942..960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          complement(1015..1587)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(1749..1952)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1953..2256)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      2335..2790
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3122..3226
                     /label=AmpR promoter
     CDS             3227..4084
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4258..4342
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
       61 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      121 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      181 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      241 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      301 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      361 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      421 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      481 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      541 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      601 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      661 gagtcagtga gcgaggaagc ggaagagtct agagtcgacc agacatgata agatacattg
      721 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      781 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ctgctttaat
      841 aagatctggt accgagctcc tgcagcccgg ggcggccgcc tcgagaagct tggatccgga
      901 ggcgtaatcg gggacgtcat aagggtacat ggtgaattcg ccctatagtg agtcgtatta
      961 caattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca ggagctgtag
     1021 gaaaaagaag aaggcatgaa catggttagc agaggggccc ggtttggact cagagtattt
     1081 tatcctcatc tcaaacagtg tatatcattg taaccataaa gagaaaggca ggatgatgac
     1141 caggatgtag ttgtttctac caataagaat atttccacgc cagccagaat ttatatgcag
     1201 aaatattcta ccttatcatt taattataac aattgttctc taaaactgtg ctgaagtaca
     1261 atataatata ccctgattgc cttgaaaaaa aagtgattag agaaagtact tacaatctga
     1321 caaataaaca aaagtgaatt taaaaattcg ttacaaatgc aagctaaagt ttaacgaaaa
     1381 agttacagaa aatgaaaaga aaataagagg agacaatggt tgtcaacaga gtagaaagtg
     1441 aaagaaacaa aattatcatg agggtccatg gtgatacaag ggacatcttc ccattctaaa
     1501 caacaccctg aaaactttgc cccctccata taacatgaat tttacaatag cgaaaaagaa
     1561 agaacaatca agggtcccca aactcaccct gaagttctca ggatcgatcc ggagcttttt
     1621 gcaaaagcct aggggtccgt tccaatgcac cgttcccggc cgcggaggct ggatcggtcc
     1681 cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc
     1741 actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     1801 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     1861 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     1921 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     1981 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     2041 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     2101 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     2161 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     2221 cagccaggcg ggccatttac cgtaagttat gtaacggact ctagctggcg taatagcgaa
     2281 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     2341 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2401 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2461 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2521 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2581 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     2641 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     2701 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2761 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     2821 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     2881 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     2941 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3001 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3061 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3241 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3421 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3601 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4321 ggtggtttgt ttgccggatc aa
//
