LOCUS       62056_22810        7170 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector pXJ070, complete sequence.
ACCESSION   MT084772
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Wang Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Single-Cell Center, Qingdao Institute of 
            Bioenergy and Bioprocess Technology, No. 189 Songling Road, Laoshan 
            District, Qingdao, Shandong Province, Qindao, Shandong 266000, China
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (20-FEB-2020) Single-Cell Center, Qingdao Institute of Bioenergy and
            Bioprocess Technology, No. 189 Songling Road, Laoshan District, 
            Qingdao, Shandong Province, Qindao, Shandong 266000, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      159..558
                     /regulatory_class="terminator"
     CDS             559..930
                     /label=BleoR
                     /note="antibiotic-binding protein"
     CDS             931..948
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      952..1459
                     /regulatory_class="terminator"
     CDS             complement(2008..2040)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             complement(2044..2061)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(2071..2088)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(2107..2129)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2144..2168)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2169..2187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2496..2573
                     /label=lacI promoter
     CDS             2574..3653
                     /label=lacI
                     /note="lac repressor"
     protein_bind    3669..3690
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             4465..4653
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4758..4900
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5086..5674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5796..6608
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(6704..7159)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggaacttcct actctcgtaa accctgtccc
      181 actcaatgta gaatacaaag caggggctgc tggcacgact ccaagcctct gcgaagcgtc
      241 aaattttcgt cttcaggcag agaacgtgct gtgtgtgtgg ctgtggagcg gagagcggct
      301 tcgtctttaa agatgccttc acccacatgg cgcgtgcccg ccctgccgat gcgtcttgat
      361 aacactacgt gttcagttac ggtgacgatg catcgtgccc ccgctgtagc cttgtgtcat
      421 tttcgttctt ctgcgacttg gaaaagggac agcgaaagcc acagaaaaca caaagaactt
      481 tcgacgactc actcaccgct cccaaccttc acccatacca cagtaacaaa acaatatcac
      541 aaccaaagac ccaagcttat ggccaagttg accagcgccg tgcctgtgtt gaccgcgagg
      601 gacgtggccg gagcggtgga gttctggacc gacaggttgg ggttctccag ggacttcgtg
      661 gaggacgact tcgccggagt ggtgagggac gacgtgacct tgttcatcag cgcggtgcag
      721 gaccaggtgg tgcctgacaa caccttggcc tgggtgtggg tgaggggatt ggacgagttg
      781 tacgccgagt ggtccgaggt ggtgtccacg aacttcaggg acgcctccgg gcctgccatg
      841 accgagatcg gagagcagcc ttgggggagg gagttcgcct tgagggaccc tgccggaaac
      901 tgcgtgcact tcgtggccga ggagcaggac caccaccacc accaccacta aaagcttacg
      961 tttttttttc ataggagaga aggcacaggc atccatgcgc tgtctcacat cacacaccta
     1021 ccacacatgt tcttgttcca tcttgatgta ttaaaagaaa aagatccaaa gcgagagagg
     1081 gatgaggcat gaattattat ttgtctgtgt ttgactatgt gattgttcgg ttgtgtgtgt
     1141 gtgtgtcctc tctcagacag gtccccaaat gaaagaagct aggtaggcag tcgcaagagt
     1201 aaccacagat aatacaatcg aaacgaccgt cccttaagag gagaagccag agaaaagaga
     1261 agtaatgagg aagcacgact tttcctgtcc ttggacttac gctggggttg agatcgtgga
     1321 ggttggattg agggagggag ggatggcaag ggtcctgttt tgcctcagac cacagcaaag
     1381 aagaaccact tgccgtgcgg gagtttccac atgttttgat gaacgcattt agcttggttt
     1441 acatttcttg acatgtattg gatccgagct cggtacccct accggaagag aaataaggcc
     1501 tttctcccgt agctgtcttc gtttgtttgt gctgattgct tggtatgaga gtgttgaatt
     1561 cctgcatcat gtttttctct gtagtccttt cctacccccg tcattttctt ttctccctgg
     1621 ttcttctttt gtcaccctta ttttacataa aattttcttt gtttatagtg agaggaaggt
     1681 agagagggga aaacaagaac aacgaacgca agcgtgtgaa aggagggcga atagaagaga
     1741 aacagatctg ttgagcattg agagtggagc cgggggaaag gcttgtgtgt tgtttttgaa
     1801 aaagttgttt aaatcacgaa tccgttagtt ctcatgtgta cctctttcac tacatgtgat
     1861 ggagaaaaca aaagtgtgag gattaattga agaaaaagaa gagttcgaca cgtcaaaccg
     1921 cccaaaagac gtcacaaaga gaacttgatt ctctttgccg tgttgatctt gtcttttccc
     1981 ccagcttttc ttgccacccg tctgcagacc catttgctgt ccaccagtca tgctagccat
     2041 atggctgccg cgcggcacca ggccgctgct gtgatgatga tgatgatggc tgctgcccat
     2101 ggtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
     2161 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
     2221 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     2281 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     2341 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     2401 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     2461 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct
     2521 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac
     2581 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
     2641 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
     2701 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
     2761 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
     2821 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
     2881 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
     2941 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
     3001 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
     3061 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
     3121 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
     3181 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
     3241 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
     3301 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
     3361 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
     3421 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     3481 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     3541 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3601 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3661 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg
     3721 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     3781 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     3841 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     3901 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     3961 ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc
     4021 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     4081 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     4141 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     4201 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     4261 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     4321 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     4381 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     4441 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc
     4501 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     4561 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     4621 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     4681 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4741 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     4801 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga
     4861 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     4921 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4981 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5041 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5101 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5161 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5221 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5281 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5341 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5401 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5461 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5521 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5581 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5641 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5701 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac
     5761 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     5821 cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     5881 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
     5941 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     6001 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     6061 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg
     6121 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     6181 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     6241 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     6301 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     6361 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     6421 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     6481 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     6541 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     6601 agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa
     6661 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat
     6721 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     6781 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     6841 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     6901 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     6961 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7021 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7081 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7141 gcgccgctac agggcgcgtc ccattcgcca
//
