LOCUS       40924_47203        4425 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pXICL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ness JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2009) DNA2.0, Inc., 1430 O'Brien Drive, Suite E, 
            Menlo Park, CA 94025, USA
REFERENCE   2  (bases 1 to 4425)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2009) DNA2.0, Inc., 1430 O'Brien Drive, Suite E, Menlo Park,
            CA 94025, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      13..1490
                     /label=ICL promoter
                     /note="ICL promoter"
                     /regulatory_class="promoter"
     CDS             1493..2200
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      2212..2508
                     /label=ICL terminator
                     /note="ICL terminator"
                     /regulatory_class="terminator"
     promoter        2510..2921
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2929..2976
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2995..3366
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      3435..3682
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgaat tctattaggc gaagaggcat ctagtagtag tggcagtggt gagaacgtgg
       61 gcgctgctat agtgaacaat ctccagtcga tggttaagaa gaagagtgac aaaccagcag
      121 tgaatgactt gtctgggtcc gtgaggaaaa gaaagaagcc cgacacaaag gacagtaacg
      181 tcaagaaacc caagaaatag gggggacctg tttagatgta taggaataaa aactccgaga
      241 tgatctcaat gtgtaatgga gttgtaatat tgcaaagggg gaaaatcaag actcaaacgt
      301 gtgtatgagt gagcgtacgt atatctccga gagtagtatg acataatgat gactgtgaat
      361 catcgtaatc tcacacaaaa accccattgt cggccatata ccacaccaag caacaccaca
      421 tatcccccgg aaaaaaaaac gtgaaaaaaa gaaacaatca aaactacaac ctactccttg
      481 atcacacagt cattgatcaa gttacagttc ctgctaggga atgaccaagg tacaaatcag
      541 caccttaatg gttagcacgc tctcttactc tctctcacag tcttccggcc cctattcaaa
      601 attctgcact tccatttgac cccagggttg ggaaacaggg ccacaaaaga aaaacccgac
      661 gtgaatgaaa aaactaagaa aagaaaaaaa attatcacac cagaaattta cctaattggg
      721 taattcccat cggtgttttt cctggattgt cgcacgcacg catgctgaaa aaagtgttcg
      781 agttttgctt ttgcctcgga gtttcacgca agtttttcga tctcggaacc ggagggcggt
      841 cgccttgttg tttgtgatgt cgtgctttgg gtgttctaat gtgctgttat tgtgctcttt
      901 ttttttcttc tttttttggt gatcatatga tattgctcgg tagattactt tcgtgtgtag
      961 gtattctttt agacgtttgg ttattgggta gatatgagag agagagagtg ggtgggggag
     1021 gagttggttg taggagggac ccctgggagg aagtgtagtt gagttttccc tgacgaatga
     1081 aaatacgttt ttgagaagat aatacaggaa aggtgtgtcg gtgaatttcc atctatccga
     1141 ggatatgagt ggaggagagt cgtgtgcgtg tggttaattt aggatcagtg gaacacacaa
     1201 agtaactaag acagagagac agagagaaaa atctggggaa gagacaaaga gtcagagtgt
     1261 gtgagttatt ctgtattgtg aaattttttt gcccaactac ataatattgc tgaaactaat
     1321 tttacttaaa aagaaaagcc aacaacgtcc ccagtaaaac ttttctataa atatcagcag
     1381 ttttcccttt cctccattcc tcttcttgtc ttttttctta ctttcccttt tttatacctt
     1441 ttcattatca tcctttataa ttgtctaacc aacaactata tatctatcaa ccatggtttc
     1501 taagggtgaa gaagacaaca tggcaatcat caaggaattt atgcgtttta aggtccatat
     1561 ggaaggctcc gttaacggcc acgagttcga gatcgaggga gaaggtgagg gtagaccata
     1621 cgaaggtact caaaccgcca agttgaaagt tacaaagggt ggtccattgc catttgcttg
     1681 ggatatcttg tccccacaat ttatgtacgg atcaaaggca tatgtcaagc atcctgccga
     1741 catcccagat tacttgaagt tatcctttcc agaaggtttt aagtgggaga gagttatgaa
     1801 ctttgaagat ggcggagttg ttactgttac tcaggactct tccttgcaag atggtgaatt
     1861 tatctataaa gtgaaattga gaggtactaa ctttccatcc gacggtccag tcatgcaaaa
     1921 gaagacaatg ggttgggagg cttcttccga aagaatgtac ccagaagacg gtgcattgaa
     1981 aggtgaaatc aagcaacgtt taaagttgaa ggacggtggt cactacgatg ccgaggtcaa
     2041 gaccacttat aaggctaaga agccagtcca attgccaggt gcttataacg ttaacatcaa
     2101 gttagatatt acttcacaca acgaagacta cacaatcgtt gaacaatatg aaagagccga
     2161 aggtagacat tctaccggcg gcatggacga gttatataag taggcggccg caagaaaaaa
     2221 gaaaaggtaa agaacttcat ttgagatgaa cttttgtata tgacttttag tttctacttt
     2281 tttttttatt tattgcttaa ttttctttat ttcaatcccc catagtttgt gtagaatata
     2341 tttattcatt ctggtaactc aaacacgtag caagctcgtt gcatctcgcc tcgtcacggg
     2401 tacagctctg gaaccaaaga caaaaaaaaa agttgatccg aaccctctcg ctattccttg
     2461 ctatgctatc cacgagatgg ggtttatcag cccaggcaag tcactaaagg atcccccaca
     2521 caccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc tcggactccg
     2581 cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaattttc cctctttctt
     2641 cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa gagaccgcct
     2701 cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga
     2761 aatttttttt tttagttttt ttctctttca gtgacctcca ttgatattta agttaataaa
     2821 cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
     2881 ttcttgttca ttagaaagaa agcatagcaa tctaatctaa ggggcggtgt tgacaattaa
     2941 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatgtct
     3001 aaattaacct ctgctgttcc agtgttaacc gcccgtgatg ttgccggtgc agtggaattt
     3061 tggactgacc gtttgggttt ctcacgtgac tttgtcgaag atgattttgc tggcgttgtg
     3121 cgtgatgacg tcactttgtt catctctgct gttcaggatc aggtcgtccc agacaacact
     3181 ttggcctggg tctgggttcg tggtttggac gaattgtacg ctgagtggag tgaagttgtg
     3241 tctacaaact ttcgtgatgc atcaggtcca gctatgaccg aaattggcga acaaccttgg
     3301 ggccgtgagt tcgctttacg tgatccagcc ggtaattgcg tgcacttcgt tgctgaggag
     3361 caagattagc acgtccgacg gcggcccacg ggtcccaggc ctcggagatc cgtccccctt
     3421 ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc ctccccccac
     3481 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
     3541 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     3601 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
     3661 acgctcgaag gctttaattt gcaagctgga gaccaacatg tgagcaaaag gccagcaaaa
     3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agatc
//
