LOCUS       40924_47193        6023 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter-probe vector pXH203, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Promoter-probe cassettes with the gusA (beta-glucuronidase) reporter
            gene and several different antibiotic resistance markers
  JOURNAL   J. Microbiol. Methods 60 (2), 281-283 (2005)
  PUBMED    15590103
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2004) Biology, Indiana University, 1001 E. 3rd 
            St., Jordan Hall, Bloomington, IN 47405, USA
REFERENCE   3  (bases 1 to 6023)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2005"; volume: "60"; issue: "2"; pages: 
            "281-283"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2004) Biology, Indiana University, 1001 E. 3rd St., Jordan 
            Hall, Bloomington, IN 47405, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             76..1914
                     /codon_start=1
                     /gene="GusA"
                     /product="beta-glucuronidase"
                     /label=GusA
                     /note="reporter; hydrolyzes beta-glucuronic acid and
                     analogs"
                     /protein_id="AAT72056.1"
                     /translation="MESLAAGQSLMLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWW
                     ESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
                     GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDEN
                     GKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDV
                     SVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI
                     RSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSH
                     YPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHL
                     QAIKELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFC
                     DAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            76..1914
                     /gene="GusA"
                     /label=GusA
     promoter        1925..1954
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2002..3189
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    3287..3337
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(3343..3361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3380..3396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3404..3420)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3428..3458)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3473..3494)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3782..4370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4544..5401)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5402..5506)
                     /label=AmpR promoter
     primer_bind     5980..5996
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5999..6017
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct ctaggatcta
       61 aggagggaaa aaaccatgga aagcttggct gcaggtcagt cccttatgtt acgtcctgta
      121 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
      181 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
      241 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc
      301 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
      361 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
      421 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
      481 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
      541 ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
      601 aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac
      661 gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag
      721 gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca
      781 actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt
      841 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
      901 ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac
      961 aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga
     1021 ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc
     1081 taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc
     1141 atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc
     1201 gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag
     1261 caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     1321 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aagtgcacgg gaatatttcg
     1381 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     1441 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     1501 cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     1561 aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     1621 gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     1681 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     1741 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     1801 aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa
     1861 cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgagagctc
     1921 atgtttgaca gcttatcatc cgattagctt taatgcggta gtttatcaca gttaaattgc
     1981 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     2041 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
     2101 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     2161 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     2221 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     2281 cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     2341 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     2401 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     2461 ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     2521 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg accgatgccc
     2581 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     2641 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     2701 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     2761 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     2821 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     2881 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     2941 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     3001 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg
     3061 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     3121 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     3181 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     3241 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccgagc tcggtacccg
     3301 gggatcctct agagtcgacc tgcaggcatg caagcttgtc tccctatagt gagtcgtatt
     3361 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3421 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3481 gctaactcac attaattgcg ttgcgctcac tgcccgcttt cgagtcggga aacctgtcgt
     3541 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3781 ttttcgatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4201 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     4261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttggc attgctacag
     4861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5521 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5581 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5641 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5701 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     5761 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     5821 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     5881 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     5941 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     6001 ttaggtgaca ctatagaata ctc
//
