LOCUS       40924_47188        5263 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter-probe vector pXH202, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Promoter-probe cassettes with the gusA (beta-glucuronidase) reporter
            gene and several different antibiotic resistance markers
  JOURNAL   J. Microbiol. Methods 60 (2), 281-283 (2005)
  PUBMED    15590103
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2004) Biology, Indiana University, 1001 E. 3rd 
            St., Jordan Hall, Bloomington, IN 47405, USA
REFERENCE   3  (bases 1 to 5263)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2005"; volume: "60"; issue: "2"; pages: 
            "281-283"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2004) Biology, Indiana University, 1001 E. 3rd St., Jordan 
            Hall, Bloomington, IN 47405, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             76..1914
                     /codon_start=1
                     /gene="GusA"
                     /product="beta-glucuronidase"
                     /label=GusA
                     /note="reporter; hydrolyzes beta-glucuronic acid and
                     analogs"
                     /protein_id="AAT72053.1"
                     /translation="MESLAAGQSLMLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWW
                     ESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
                     GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDEN
                     GKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDV
                     SVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI
                     RSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSH
                     YPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHL
                     QAIKELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFC
                     DAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            76..1914
                     /gene="GusA"
                     /label=GusA
     promoter        1933..1961
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2288..2521
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    2527..2577
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(2583..2601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2620..2636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2644..2660)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2668..2698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2713..2734)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3022..3610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3784..4641)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4642..4746)
                     /label=AmpR promoter
     primer_bind     5220..5236
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5239..5257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct ctaggatcta
       61 aggagggaaa aaaccatgga aagcttggct gcaggtcagt cccttatgtt acgtcctgta
      121 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
      181 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
      241 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc
      301 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
      361 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
      421 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
      481 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
      541 ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
      601 aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac
      661 gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag
      721 gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca
      781 actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt
      841 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
      901 ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac
      961 aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga
     1021 ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc
     1081 taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc
     1141 atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc
     1201 gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag
     1261 caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     1321 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aagtgcacgg gaatatttcg
     1381 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     1441 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     1501 cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     1561 aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     1621 gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     1681 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     1741 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     1801 aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa
     1861 cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgagagctc
     1921 gcacgaaccc agttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
     1981 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2041 gttttcatgg cttgttatga ctgttttttt gtacagtcta gcctcgggca tccaagctag
     2101 ctaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg aacagcaacg atgttacgca
     2161 gcagggtagt cgccctaaaa caaagttagg cagccgttgt gctggtgctt tctagtagtt
     2221 gttgtggggt aggcagtcag aggtcgattt gcttgtcgcc ataatagatt cacaagaagg
     2281 attcgacatg ggtcaaagta gcgatgaagc caacgctccc gttgcagggc agtttgcgct
     2341 tcccctgagt gccacctttg gcttagggga tcgcgtacgc aagaaatctg gtgccgcttg
     2401 gcagggtcaa gtcgtcggtt ggtattgcac aaaactcact cctgaaggct atgcggtcga
     2461 gtccgaatcc cacccaggct cagtgcaaat ttatcctgtg gctgcacttg aacgtgtggc
     2521 ctaagcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgtc
     2581 tccctatagt gagtcgtatt agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     2641 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2701 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2761 cgagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2821 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3001 aaaggccgcg ttgctggcgt ttttcgatag gctccgcccc cctgacgagc atcacaaaaa
     3061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3421 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4081 cgttgttggc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4381 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4741 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4801 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     4861 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4921 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     4981 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     5041 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     5101 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5161 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     5221 taaaacgacg gccagtgaat ttaggtgaca ctatagaata ctc
//
