LOCUS       40924_47178        5895 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter-probe vector pXH200, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Promoter-probe cassettes with the gusA (beta-glucuronidase) reporter
            gene and several different antibiotic resistance markers
  JOURNAL   J. Microbiol. Methods 60 (2), 281-283 (2005)
  PUBMED    15590103
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   He X, Bhateja M, Fuqua C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2004) Biology, Indiana University, 1001 E. 3rd 
            St., Jordan Hall, Bloomington, IN 47405, USA
REFERENCE   3  (bases 1 to 5895)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5895)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2005"; volume: "60"; issue: "2"; pages: 
            "281-283"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2004) Biology, Indiana University, 1001 E. 3rd St., Jordan 
            Hall, Bloomington, IN 47405, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             76..1914
                     /codon_start=1
                     /gene="GusA"
                     /product="beta-glucuronidase"
                     /label=GusA
                     /note="reporter; hydrolyzes beta-glucuronic acid and
                     analogs"
                     /protein_id="AAT72047.1"
                     /translation="MESLAAGQSLMLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWW
                     ESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
                     GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDEN
                     GKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDV
                     SVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI
                     RSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSH
                     YPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHL
                     QAIKELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFC
                     DAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            76..1914
                     /gene="GusA"
                     /label=GusA
     CDS             2044..2856
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    3159..3209
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(3215..3233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3252..3268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3276..3292)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3300..3330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3345..3366)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3654..4242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4416..5273)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5274..5378)
                     /label=AmpR promoter
     primer_bind     5852..5868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5871..5889
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct ctaggatcta
       61 aggagggaaa aaaccatgga aagcttggct gcaggtcagt cccttatgtt acgtcctgta
      121 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
      181 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
      241 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc
      301 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
      361 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
      421 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
      481 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
      541 ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
      601 aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac
      661 gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag
      721 gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca
      781 actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt
      841 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
      901 ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac
      961 aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga
     1021 ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc
     1081 taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc
     1141 atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc
     1201 gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag
     1261 caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     1321 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aagtgcacgg gaatatttcg
     1381 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     1441 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     1501 cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     1561 aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     1621 gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     1681 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     1741 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     1801 aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa
     1861 cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgagagctc
     1921 gggggggggg gggaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat
     1981 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2041 gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg
     2101 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2161 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2221 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2281 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2341 gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2401 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2461 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2521 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2581 aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2641 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2701 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2761 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2821 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2881 taacactggc agagcattac gctgacttga cgggacggcg gctttgttga ataaatcgaa
     2941 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     3001 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     3061 gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac
     3121 cttcttcacg aggcagacct cagcgccccc ccccccccga gctcggtacc cggggatcct
     3181 ctagagtcga cctgcaggca tgcaagcttg tctccctata gtgagtcgta ttagagcttg
     3241 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3361 acattaattg cgttgcgctc actgcccgct ttcgagtcgg gaaacctgtc gtgccagctg
     3421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     3481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3601 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttcgat
     3661 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3721 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3781 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3901 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3961 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4021 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4081 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4141 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4261 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4321 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4381 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4501 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4621 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg gcattgctac aggcatcgtg
     4741 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4801 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4861 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4921 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4981 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5041 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5101 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5161 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5221 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5281 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5341 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5401 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     5461 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     5521 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     5581 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     5641 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     5701 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     5761 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     5821 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga atttaggtga
     5881 cactatagaa tactc
//
