LOCUS       40924_47153        3239 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXEN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK 
            regulation
  JOURNAL   J. Biol. Chem. 283 (23), 15672-15680 (2008)
  PUBMED    18397886
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2007) Department of Physiology, Semmelweis 
            University, Puskin str. 9, Budapest 1088, Hungary
REFERENCE   3  (bases 1 to 3239)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3239)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2008"; volume: "283"; issue: "23"; pages: 
            "15672-15680"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-NOV-2007) Department of Physiology, Semmelweis University, 
            Puskin str. 9, Budapest 1088, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           27..60
                     /label=derived from Xenopus globin
                     /note="derived from Xenopus globin"
     misc_feature    61..114
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           132..257
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     primer_bind     complement(341..357)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(394..412)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(433..449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(457..473)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(481..511)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(526..547)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(835..1423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1597..2454)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2455..2559)
                     /label=AmpR promoter
     rep_origin      2586..3041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3182..3198
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3208..3226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgagcttgat ttaggtgaca ctatagaagc tcagaataaa cgctcaactt tggcagatcc
       61 gaattcgaac ctaaggaagc ttcgcgatcc ggaccttggc atgcagatct cgagtgatct
      121 ggttaccact aaaccagcct caagaacacc cgaatggagt ctctaagcta cataatacca
      181 acttacactt tacaaaatgt tgtcccccaa aatgtagcca ttcgtatctg ctcctaataa
      241 aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa aaggaaaaaa aaaaaaaaaa
      301 cccccccccc atcactagtg cggccgcctg cagcccgggg gatccactag ttctagagcg
      361 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1261 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1441 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1501 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1561 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1621 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1681 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1741 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1801 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1861 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1921 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1981 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2041 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2101 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2161 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2221 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2281 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2341 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2401 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2461 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2521 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2581 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     2641 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     2701 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     2761 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     2821 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     2881 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     2941 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3001 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
     3061 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3121 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3181 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtac
//
