LOCUS       40924_47148        3563 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXEN-pQ108, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK 
            regulation
  JOURNAL   J. Biol. Chem. 283 (23), 15672-15680 (2008)
  PUBMED    18397886
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Czirjak G, Vuity D, Enyedi P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2007) Department of Physiology, Semmelweis 
            University, Puskin str. 9, Budapest 1088, Hungary
REFERENCE   3  (bases 1 to 3563)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3563)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2008"; volume: "283"; issue: "23"; pages: 
            "15672-15680"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-NOV-2007) Department of Physiology, Semmelweis University, 
            Puskin str. 9, Budapest 1088, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           27..60
                     /label=derived from Xenopus globin
                     /note="derived from Xenopus globin"
     misc_feature    61..94
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    95..418
                     /label=polypeptide linker
                     /note="polypeptide linker"
     misc_feature    419..438
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           456..581
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     primer_bind     complement(665..681)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(718..736)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(757..773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(781..797)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(805..835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(850..871)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1159..1747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1921..2778)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2779..2883)
                     /label=AmpR promoter
     rep_origin      2910..3365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3506..3522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3532..3550
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgagcttgat ttaggtgaca ctatagaagc tcagaataaa cgctcaactt tggcagatcc
       61 gaattcgaac ctaaggaagc ttcgcgatcc ggaccttgag catcagcagc aacagcaaca
      121 gcagcagcaa cttgagcatc agcagcaaca gcaacagcag cagcaacttg agcatcagca
      181 gcaacagcaa cagcagcagc aacttgagca tcagcagcaa cagcaacagc agcagcaact
      241 tgagcatcag cagcaacagc aacagcagca gcaacttgag catcagcagc aacagcaaca
      301 gcagcagcaa cttgagcatc agcagcaaca gcaacagcag cagcaacttg agcatcagca
      361 gcaacagcaa cagcagcagc aacttgagca tcagcagcaa cagcaacagc agcagcaact
      421 tggcatgcag atctcgagtg atctggttac cactaaacca gcctcaagaa cacccgaatg
      481 gagtctctaa gctacataat accaacttac actttacaaa atgttgtccc ccaaaatgta
      541 gccattcgta tctgctccta ataaaaagaa agtttcttca cattctaaaa aaaaaaaaaa
      601 aaaaaaggaa aaaaaaaaaa aaaacccccc ccccatcact agtgcggccg cctgcagccc
      661 gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct
      721 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      781 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      841 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      901 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      961 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1561 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     1621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1921 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1981 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2041 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2101 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2161 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2221 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2281 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2341 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2401 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2461 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2521 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2581 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2641 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2701 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2761 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2821 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2881 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     2941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3361 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     3421 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     3481 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     3541 tcactatagg gcgaattggg tac
//
