LOCUS       40924_47143        5332 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXCS-HAStrep, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Witte CP, Noel LD, Gielbert J, Parker JE, Romeis T.
  TITLE     Rapid one-step protein purification from plant material using the 
            eight-amino acid StrepII epitope
  JOURNAL   Plant Mol. Biol. 55 (1), 135-147 (2004)
  PUBMED    15604670
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Witte C-P., Noel L, Parker JE, Romeis T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2003) Plant-Microbe Interactions, 
            Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg
            10, Koeln D-50829, Germany
REFERENCE   3  (bases 1 to 5332)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2004"; volume: "55"; issue: "1"; pages: "135-147"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2003) Plant-Microbe Interactions, Max-Planck-Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..48
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             61..84
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     regulatory      90..306
                     /label=pA35S
                     /note="pA35S"
                     /regulatory_class="terminator"
     misc_feature    377..401
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      513..1222
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1296..1387
                     /label=AmpR promoter
     CDS             1388..2245
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2419..3007
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3319..3343
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3437..3689)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3712..4260)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4303..4482)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4626..5299
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     5302..5318
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcctgc agcccggggg ttatccatac gatgttccag attatgctgt cggcgccggt
       61 tggtctcatc ctcaatttga aaaataagtc tagagtccgc aaaaatcacc agtctctctc
      121 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
      181 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
      241 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
      301 gtgaccgggc ggccgccacc gcggtggagg gggatcagat tgtcgtttcc cgccttcagt
      361 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
      421 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
      481 gtgtacatca ccgacgagca aggcaagacc gagcgccttt ccgacgctca ccgggctggt
      541 tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc
      601 gaagccgtgt gcgagacacc ggccgccggc gttgtggata cctcgcggaa aacttggccc
      661 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
      721 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
      781 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
      841 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
      901 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
      961 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
     1021 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
     1081 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
     1141 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
     1201 ggcctcaccc caaaaatggc agcgctggca gtccttgcca ttgccgggat cggggcagta
     1261 acgggatggg cgatcagccc gacaagctac ccctatttgt ttatttttct aaatacattc
     1321 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1381 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1441 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1501 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1561 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1621 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1681 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1741 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1801 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1861 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1921 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1981 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2041 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2101 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2161 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2221 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2521 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3061 tcacatggac tctagctaga ggatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     3121 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     3181 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     3241 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     3301 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     3361 acttaataac acattgcgga cgtttttaat gatcgaatac taacgtctct accagatatc
     3421 agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     3481 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     3541 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     3601 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     3661 aaactttatt gccaaatgtt tgaacgatct gcttgactct aggggtcatc agatttcggt
     3721 gacgggcagg accggacggg gcggcaccgg caggctgaag tccagctgcc agaaacccac
     3781 gtcatgccag ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg gggcatatcc
     3841 gagcgcctcg tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag cgaccacgct
     3901 cttgaagccc tgtgcctcca gggacttcag caggtgggtg tagagcgtgg agcccagtcc
     3961 cgtccgctgg tggcgggggg agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt
     4021 gcgtgccttc caggggcccg cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac
     4081 gagccaggga tagcgctccc gcagacggac gaggtcgtcc gtccactcct gcggttcctg
     4141 cggctcggta cggaagttga ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac
     4201 cgccggcatg tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat
     4261 ggtagatccc cctcgatcga gttgagagtg aatatgagac tctaattgga taccgagggg
     4321 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     4381 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     4441 ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     4501 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     4561 tgatcagatt gtcgtttccc gccttcggtt tgggcgcgcc cggtctcaga agaccagagg
     4621 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     4681 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat
     4741 cattgcgata aaggaaaggc tatcgttcaa gaatgcctct accgacagtg gtcccaaaga
     4801 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4861 gcaagtggat tgatgtgata acatggtgga gcacgacact ctcgtctact ccaagaatat
     4921 caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     4981 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     5041 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     5101 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
     5161 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     5221 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     5281 catttcattt ggagaggacc tcgaggtcga cggtatcgat aagcttgata tc
//
