LOCUS       62056_22795        4059 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    116..250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(550..1138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1312..2169)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2170..2274)
                     /label=AmpR promoter
     terminator      2443..2470
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2547..3081
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     intron          3175..4001
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
ORIGIN
        1 gaattcattg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
       61 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      241 tgtctggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      301 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      361 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      421 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      481 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgacctc gggccgcgtt
      541 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      601 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      661 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      721 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      781 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      841 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      901 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      961 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1021 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1081 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1141 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1201 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1261 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1321 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1381 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1441 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1501 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1561 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1621 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1681 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1741 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1801 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1861 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1921 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1981 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2041 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2101 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2161 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2221 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2281 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2341 aaataggcgt atcacgaggc cctgatggct ctttgcggca cccatcgttc gtaatgttcc
     2401 gtggcaccga ggacaaccct caagagaaaa tgtaatcaca ctggctcacc ttcgggtggg
     2461 cctttctgcg tttataagga gacactttat gtttaagaag gttggtaaat tccttgcggc
     2521 tttggcagcc aagctagcgg ccgctgaggc gcgcctactg agtcattagg gactttccaa
     2581 tgggttttgc ccagtacata aggtcaatag gggtgaatca acaggaaagt cccattggag
     2641 ccaagtacac tgagtcaata gggactttcc attgggtttt gcccagtaca aaaggtcaat
     2701 agggggtgag tcaatgggtt tttcccatta ttggcacgta cataaggtca ataggggtga
     2761 gtcattgggt ttttccagcc aatttaatta aaacgccatg tactttccca ccattgacgt
     2821 caatgggcta ttgaaactaa tgcaacgtga cctttaaacg gtactttccc atagctgatt
     2881 aatgggaaag taccgttctc gagccaatac acgtcaatgg gaagtgaaag ggcagccaaa
     2941 acgtaacacc gccccggttt tcccctggaa attccatatt ggcacgcatt ctattggctg
     3001 agctgcgttc tacgtgggta taagaggcgc gaccagcgtc ggtaccgtcg cagtcttcgg
     3061 tctgaccacc gtagaacgca gcctcaggac ctccatagaa gacaccggga ccgatccagc
     3121 ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt
     3181 accgcctata gagtctatag gcccaccccc ttggcttctt atgcatgcta tactgttttt
     3241 ggcttggggt ctatacaccc ccgcttcctc atgttatagg tgatggtata gcttagccta
     3301 taggtgtggg ttattgacca ttattgacca ctcccctatt ggtgacgata ctttccatta
     3361 ctaatccata acatggctct ttgccacaac tctctttatt ggctatatgc caatacactg
     3421 tccttcagag actgacacgg actctgtatt tttacaggat ggggtctcat ttattattta
     3481 caaattcaca tatacaacac caccgtcccc agtgcccgca gtttttatta aacataacgt
     3541 gggatctcca cgcgaatctc gggtacgtgt tccggacatg ggctcttctc cggtagcggc
     3601 ggagcttcta catccgagcc ctgctcccat gcctccagcg actcatggtc gctcggcagc
     3661 tccttgctcc taacagtgga ggccagactt aggcacagca cgatgcccac caccaccagt
     3721 gtgccgcaca aggccgtggc ggtagggtat gtgtctgaaa atgagctcgg ggagcgggct
     3781 tgcaccgctg acgcatttgg aagacttaag gcagcggcag aagaagatgc aggcagctga
     3841 gttgttgtgt tctgataaga gtcagaggta actcccgttg cggtgctgtt aacggtggag
     3901 ggcagtgtag tctgagcagt actcgttgct gccgcgcgcg ccaccagaca taatagctga
     3961 cagactaaca gactgttcct ttccatgggt cttttctgca gtcaccgtcc ttgacacgaa
     4021 gcttcgcgac gtacgttcga acccgggcac gtgcggacc
//
