LOCUS       V014286                 6934 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014286
VERSION     V014286
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    116..237
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(999..1587)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1761..2618)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2619..2723)
                     /label="AmpR promoter"
     terminator      2892..2919
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     promoter        3002..3331
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3370..4488
                     /gene="GLUL"
                     /label="Glutamine synthetase"
                     /note="Glutamine synthetase from Acomys cahirinus.
                     Accession#: Q9QY94"
     intron          4623..4688
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4818..4838
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5263..5397
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        5422..5956
                     /label="mCMV promoter"
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     intron          6050..6876
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
ORIGIN
        1 gaattcattg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
       61 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      241 tgtctggcgg ccgcgacctg caggcgcaga actggtaggt atggaagatc cctcgagatc
      301 cattgtgctg gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa
      361 atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg
      421 cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct
      481 gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt
      541 tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca
      601 ctttatcact gataaacata atatgtccac caacttatca gtgataaaga atccgcgcca
      661 gcacaatgga tctcgaggtc gagggatctc tagaggatcc tctacgccgg acgcatcgtg
      721 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
      781 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
      841 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
      901 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
      961 aagggagagc gtcgacctcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2761 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctgatggctc
     2821 tttgcggcac ccatcgttcg taatgttccg tggcaccgag gacaaccctc aagagaaaat
     2881 gtaatcacac tggctcacct tcgggtgggc ctttctgcgt ttataaggag acactttatg
     2941 tttaagaagg ttggtaaatt ccttgcggct ttggcagcca agctagatcc ggctgtggaa
     3001 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3061 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3121 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3301 aggctttttt ggaggcctag gcttttgcaa aaagctagct tggggccacc gctcagagca
     3361 ccttccacca tggccacctc agcaagttcc cacttgaaca aaaacatcaa gcaaatgtac
     3421 ttgtgcctgc cccagggtga gaaagtccaa gccatgtata tctgggttga tggtactgga
     3481 gaaggactgc gctgcaaaac ccgcaccctg gactgtgagc ccaagtgtgt agaagagtta
     3541 cctgagtgga attttgatgg ctctagtacc tttcagtctg agggctccaa cagtgacatg
     3601 tatctcagcc ctgttgccat gtttcgggac cccttccgca gagatcccaa caagctggtg
     3661 ttctgtgaag ttttcaagta caaccggaag cctgcagaga ccaatttaag gcactcgtgt
     3721 aaacggataa tggacatggt gagcaaccag cacccctggt ttggaatgga acaggagtat
     3781 actctgatgg gaacagatgg gcaccctttt ggttggcctt ccaatggctt tcctgggccc
     3841 caaggtccgt attactgtgg tgtgggcgca gacaaagcct atggcaggga tatcgtggag
     3901 gctcactacc gcgcctgctt gtatgctggg gtcaagatta caggaacaaa tgctgaggtc
     3961 atgcctgccc agtgggagtt ccaaatagga ccctgtgaag gaatccgcat gggagatcat
     4021 ctctgggtgg cccgtttcat cttgcatcga gtatgtgaag actttggggt aatagcaacc
     4081 tttgacccca agcccattcc tgggaactgg aatggtgcag gctgccatac caactttagc
     4141 accaaggcca tgcgggagga gaatggtctg aagcacatcg aggaggccat cgagaaacta
     4201 agcaagcggc accggtacca cattcgagcc tacgatccca aggggggcct ggacaatgcc
     4261 cgtcgtctga ctgggttcca cgaaacgtcc aacatcaacg acttttctgc tggtgtcgcc
     4321 aatcgcagtg ccagcatccg cattccccgg actgtcggcc aggagaagaa aggttacttt
     4381 gaagaccgcc gcccctctgc caattgtgac ccctttgcag tgacagaagc catcgtccgc
     4441 acatgccttc tcaatgagac tggcgacgag cccttccaat acaaaaacta attagacttt
     4501 gagtgatctt gagcctttcc tagttcatcc caccccgccc cagagagatc tttgtgaagg
     4561 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     4621 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     4681 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     4741 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     4801 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     4861 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     4921 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     4981 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     5041 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     5101 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     5161 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5221 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     5281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctct
     5401 agcttcgtgt caaggacggt gaggcgcgcc tactgagtca ttagggactt tccaatgggt
     5461 tttgcccagt acataaggtc aataggggtg aatcaacagg aaagtcccat tggagccaag
     5521 tacactgagt caatagggac tttccattgg gttttgccca gtacaaaagg tcaatagggg
     5581 gtgagtcaat gggtttttcc cattattggc acgtacataa ggtcaatagg ggtgagtcat
     5641 tgggtttttc cagccaattt aattaaaacg ccatgtactt tcccaccatt gacgtcaatg
     5701 ggctattgaa actaatgcaa cgtgaccttt aaacggtact ttcccatagc tgattaatgg
     5761 gaaagtaccg ttctcgagcc aatacacgtc aatgggaagt gaaagggcag ccaaaacgta
     5821 acaccgcccc ggttttcccc tggaaattcc atattggcac gcattctatt ggctgagctg
     5881 cgttctacgt gggtataaga ggcgcgacca gcgtcggtac cgtcgcagtc ttcggtctga
     5941 ccaccgtaga acgcagcctc aggacctcca tagaagacac cgggaccgat ccagcctccg
     6001 cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc
     6061 ctatagagtc tataggccca cccccttggc ttcttatgca tgctatactg tttttggctt
     6121 ggggtctata cacccccgct tcctcatgtt ataggtgatg gtatagctta gcctataggt
     6181 gtgggttatt gaccattatt gaccactccc ctattggtga cgatactttc cattactaat
     6241 ccataacatg gctctttgcc acaactctct ttattggcta tatgccaata cactgtcctt
     6301 cagagactga cacggactct gtatttttac aggatggggt ctcatttatt atttacaaat
     6361 tcacatatac aacaccaccg tccccagtgc ccgcagtttt tattaaacat aacgtgggat
     6421 ctccacgcga atctcgggta cgtgttccgg acatgggctc ttctccggta gcggcggagc
     6481 ttctacatcc gagccctgct cccatgcctc cagcgactca tggtcgctcg gcagctcctt
     6541 gctcctaaca gtggaggcca gacttaggca cagcacgatg cccaccacca ccagtgtgcc
     6601 gcacaaggcc gtggcggtag ggtatgtgtc tgaaaatgag ctcggggagc gggcttgcac
     6661 cgctgacgca tttggaagac ttaaggcagc ggcagaagaa gatgcaggca gctgagttgt
     6721 tgtgttctga taagagtcag aggtaactcc cgttgcggtg ctgttaacgg tggagggcag
     6781 tgtagtctga gcagtactcg ttgctgccgc gcgcgccacc agacataata gctgacagac
     6841 taacagactg ttcctttcca tgggtctttt ctgcagtcac cgtccttgac acgaagcttc
     6901 gcgacgtacg ttcgaacccg ggcacgtgcg gacc
//
