LOCUS       40924_47113       10266 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pXB173-lux, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Bina XR, Miller MA, Bina JE.
  TITLE     Construction of a bioluminescence reporter plasmid for Francisella 
            tularensis
  JOURNAL   Plasmid 64 (3), 156-161 (2010)
  PUBMED    20620161
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Bina JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2010) Molecular Sciences, The University of 
            Tennessee HSC, Memphis, TN 38163, USA
REFERENCE   3  (bases 1 to 10266)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2010"; volume: "64"; issue: "3"; pages: "156-161"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAR-2010) Molecular Sciences, The University of Tennessee HSC, 
            Memphis, TN 38163, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(2..185)
                     /label=repA promoter
                     /note="repA promoter"
                     /regulatory_class="promoter"
     regulatory      186..372
                     /label=orf5 promoter
                     /note="orf5 promoter"
                     /regulatory_class="promoter"
     CDS             418..1230
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1319..1707)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            1878..1987
                     /label=oriT
                     /note="incP origin of transfer"
     regulatory      2088..2305
                     /label=Francisella tularensis gro promoter
                     /note="Francisella tularensis gro promoter"
                     /regulatory_class="promoter"
     CDS             2382..3821
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             3837..4757
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             4809..5888
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             5906..6886
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             7068..8177
                     /label=LuxE
                     /note="LuxE"
     CDS             complement(8823..9245)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /protein_id="ADI49667.1"
                     /translation="MNIEIFKFSKKHKKINKKLIDLTKVKQVVTTGYSFIVVYQRGRKI
                     ELSYESSYWHAKKDLDNSNSDVGITYSYIKKDKRGPCHKDNYLRDSKGRLLKDWGKIEE
                     LHEKYPKIGKIWYISREETDKLLKDFTYKNGNLIMV"
     gene            complement(8823..9245)
                     /gene="repB"
                     /label=repB
ORIGIN
        1 tagaactaga gtcattatct cgatatacaa attctataaa acttctatta gtaaaacaac
       61 tacttcataa aaaaaagtag ttttaacgat acaaaaagta gttttaaatt caaaaagtga
      121 tacaaaaagt agttttaaat tcaaaaagtg atacaaaaag tagttttaaa ttttttaaaa
      181 aagtgcttca aagccttatg tagcaatact tacagaggat taaaaaaaaa tctgacaata
      241 tataaagaga atatataaag agaatatctt aggggatttt aaaaaaatcc cacagactca
      301 aagacttttt tgacttttta aatcctagaa actatacttt aagtacttat ttaagtacat
      361 ggatttagat tatgcaaacc gttaaggatc aaggcgcgcc acgcgtagga gtttgttatg
      421 agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct
      481 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      541 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
      601 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
      661 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
      721 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
      781 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
      841 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
      901 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
      961 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1021 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     1081 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     1141 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     1201 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     1261 tggcagagca ttacgctgac ttgacgcgtg ccgatgatca acgcgatcta ctatgccatg
     1321 tcagccgtta agtgttcctg tgtcactcaa aattgctttg agaggctcta agggcttctc
     1381 agtgcgttac atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc
     1441 ttatatattc ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat
     1501 ttatattaat tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta
     1561 cgttagccat gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc
     1621 ttagtacgtt aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca
     1681 acaggttgaa ctgctgatct tcagatcctc tacgccggac gcatcgtggc cggatcaatt
     1741 ccgttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     1801 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     1861 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     1921 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     1981 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
     2041 accaggaagg gcagcccacc tatcacggaa ttgatccccg atatccctaa agttaaaatc
     2101 gagagcttgt ttgacaaaaa aacaaaaaaa tttcttgaaa attttttttt tgactcaata
     2161 tctagacttg caagagcttg gaactttgag attgttctaa gatgcataca aattcaaaat
     2221 gcttaaacaa aaataattta acaaaggagt aagattgtta tgaacattcg tccattacaa
     2281 gatagagtat tagttcgtcg tgcaggtacc gagctcggat ccactagtaa cggccgccag
     2341 tgtgctggaa ttcgcccttg gatccattaa aggaggcaaa tatgactaaa aaaatttcat
     2401 tcattattaa cggccaggtt gaaatctttc ccgaaagtga tgatttagtg caatccatta
     2461 attttggtga taatagtgtt tacctgccaa tattgaatga ctctcatgta aaaaacatta
     2521 ttgattgtaa tggaaataac gaattacggt tgcataacat tgtcaatttt ctctatacgg
     2581 tagggcaaag atggaaaaat gaagaatact caagacgcag gacatacatt cgtgacttaa
     2641 aaaaatatat gggatattca gaagaaatgg ctaagctaga ggccaattgg atatctatga
     2701 ttttatgttc taaaggcggc ctttatgatg ttgtagaaaa tgaacttggt tctcgccata
     2761 tcatggatga atggctacct caggatgaaa gttatgttcg ggcttttccg aaaggtaaat
     2821 ctgtacatct gttggcaggt aatgttccat tatctgggat catgtctata ttacgcgcaa
     2881 ttttaactaa gaatcagtgt attataaaaa catcgtcaac cgatcctttt accgctaatg
     2941 cattagcgtt aagttttatt gatgtagacc ctaatcatcc gataacgcgc tctttatctg
     3001 ttatatattg gccccaccaa ggtgatacat cactcgcaaa agaaattatg cgacatgcgg
     3061 atgttattgt cgcttgggga gggccagatg cgattaattg ggcggtagag catgcgccat
     3121 cttatgctga tgtgattaaa tttggttcta aaaagagtct ttgcattatc gataatcctg
     3181 ttgatttgac gtccgcagcg acaggtgcgg ctcatgatgt ttgtttttac gatcagcgag
     3241 cttgtttttc tgcccaaaac atatattaca tgggaaatca ttatgaggaa tttaagttag
     3301 cgttgataga aaaacttaat ctatatgcgc atatattacc gaatgccaaa aaagattttg
     3361 atgaaaaggc ggcctattct ttagttcaaa aagaaagctt gtttgctgga ttaaaagtag
     3421 aggtggatat tcatcaacgt tggatgatta ttgagtcaaa tgcaggtgtg gaatttaatc
     3481 aaccacttgg cagatgtgtg taccttcatc acgtcgataa tattgagcaa atattgcctt
     3541 atgttcaaaa aaataagacg caaaccatat ctatttttcc ttgggagtca tcatttaaat
     3601 atcgagatgc gttagcatta aaaggtgcgg aaaggattgt agaagcagga atgaataaca
     3661 tatttcgagt tggtggatct catgacggaa tgagaccgtt gcaacgatta gtgacatata
     3721 tttctcatga aaggccatct aactatacgg ctaaggatgt tgcggttgaa atagaacaga
     3781 ctcgattcct ggaagaagat aagttccttg tatttgtccc ataataggta aaaagtatgg
     3841 aaaatgaatc aaaatataaa accatcgacc acgttatttg tgttgaagga aataaaaaaa
     3901 ttcatgtttg ggaaacgctg ccagaagaaa acagcccaaa gagaaagaat gccattatta
     3961 ttgcgtctgg ttttgcccgc aggatggatc attttgctgg tctggcggaa tatttatcgc
     4021 ggaatggatt tcatgtgatc cgctatgatt cgcttcacca cgttggattg agttcaggga
     4081 caattgatga atttacaatg tctataggaa agcagagctt gttagcagtg gttgattggt
     4141 taactacacg aaaaataaat aacttcggta tgttggcttc aagcttatct gcgcggatag
     4201 cttatgcaag cctatctgaa atcaatgctt cgtttttaat caccgcagtc ggtgttgtta
     4261 acttaagata ttctcttgaa agagctttag ggtttgatta tctcagtcta cccattaatg
     4321 aattgccgaa taatctagat tttgaaggcc ataaattggg tgctgaagtc tttgcgagag
     4381 attgtcttga ttttggttgg gaagatttag cttctacaat taataacatg atgtatcttg
     4441 atataccgtt tattgctttt actgcaaata acgataattg ggtcaagcaa gatgaagtta
     4501 tcacattgtt atcaaatatt cgtagtaatc gatgcaagat atattctttg ttaggaagtt
     4561 cgcatgactt gagtgaaaat ttagtggtcc tgcgcaattt ttatcaatcg gttacgaaag
     4621 ccgctatcgc gatggataat gatcatctgg atattgatgt tgatattact gaaccgtcat
     4681 ttgaacattt aactattgcg acagtcaatg aacgccgaat gagaattgag attgaaaatc
     4741 aagcaatttc tctgtcttaa aatctattga gatattctat cactcaaata gcaatataag
     4801 gactctctat gaaatttgga aactttttgc ttacatacca acctccccaa ttttctcaaa
     4861 cagaggtaat gaaacgtttg gttaaattag gtcgcatctc tgaggagtgt ggttttgata
     4921 ccgtatggtt actggagcat catttcacgg agtttggttt gcttggtaac ccttatgtcg
     4981 ctgctgcata tttacttggc gcgactaaaa aattgaatgt aggaactgcc gctattgttc
     5041 ttcccacagc ccatccagta cgccaacttg aagatgtgaa tttattggat caaatgtcaa
     5101 aaggacgatt tcggtttggt atttgccgag ggctttacaa caaggacttt cgcgtattcg
     5161 gcacagatat gaataacagt cgcgccttag cggaatgctg gtacgggctg ataaagaatg
     5221 gcatgacaga gggatatatg gaagctgata atgaacatat caagttccat aaggtaaaag
     5281 taaaccccgc ggcgtatagc agaggtggcg caccggttta tgtggtggct gaatcagctt
     5341 cgacgactga gtgggctgct caatttggcc taccgatgat attaagttgg attataaata
     5401 ctaacgaaaa gaaagcacaa cttgagcttt ataatgaagt ggctcaagaa tatgggcacg
     5461 atattcataa tatcgaccat tgcttatcat atataacatc tgtagatcat gactcaatta
     5521 aagcgaaaga gatttgccgg aaatttctgg ggcattggta tgattcttat gtgaatgcta
     5581 cgactatttt tgatgattca gaccaaacaa gaggttatga tttcaataaa gggcagtggc
     5641 gtgactttgt attaaaagga cataaagata ctaatcgccg tattgattac agttacgaaa
     5701 tcaatcccgt gggaacgccg caggaatgta ttgacataat tcaaaaagac attgatgcta
     5761 caggaatatc aaatatttgt tgtggatttg aagctaatgg aacagtagac gaaattattg
     5821 cttccatgaa gctcttccag tctgatgtca tgccatttct taaagaaaaa caacgttcgc
     5881 tattatatta gctaaggaga aagaaatgaa atttggattg ttcttcctta acttcatcaa
     5941 ttcaacaact gttcaagaac aaagtatagt tcgcatgcag gaaataacgg agtatgttga
     6001 taagttgaat tttgaacaga ttttagtgta tgaaaatcat ttttcagata atggtgttgt
     6061 cggcgctcct ctgactgttt ctggttttct gctcggttta acagagaaaa ttaaaattgg
     6121 ttcattaaat cacatcatta caactcatca tcctgtccgc atagcggagg aagcttgctt
     6181 attggatcag ttaagtgaag ggagatttat tttagggttt agtgattgcg aaaaaaaaga
     6241 tgaaatgcat ttttttaatc gcccggttga atatcaacag caactatttg aagagtgtta
     6301 tgaaatcatt aacgatgctt taacaacagg ctattgtaat ccagataacg atttttatag
     6361 cttccctaaa atatctgtaa atccccatgc ttatacgcca ggcggacctc ggaaatatgt
     6421 aacagcaacc agtcatcata ttgttgagtg ggcggccaaa aaaggtattc ctctcatctt
     6481 taagtgggat gattctaatg atgttagata tgaatatgct gaaagatata aagccgttgc
     6541 ggataaatat gacgttgacc tatcagagat agaccatcag ttaatgatat tagttaacta
     6601 taacgaagat agtaataaag ctaaacaaga gacgcgtgca tttattagtg attatgttct
     6661 tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa gaaataattg cagaaaacgc
     6721 tgtcggaaat tatacggagt gtataactgc ggctaagttg gcaattgaaa agtgtggtgc
     6781 gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa aaaatgtaat
     6841 caatattgtt gatgataata ttaagaagta ccacatggaa tatacctaat agatttcgag
     6901 ttgcagcgag gcggcaagtg aacgaatccc caggagcata gataactatg tgactggggt
     6961 gagtgaaagc agccaacaaa gcagcagctt gaaagatgaa gggtataaaa gagtatgaca
     7021 gcagtgctgc catactttct aatattatct tgaggagtaa aacaggtatg acttcatatg
     7081 ttgataaaca agaaattaca gcaagctcag aaattgatga tttgattttt tcgagcgatc
     7141 cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa gaaacttgtg cttgatgcat
     7201 ttcgtaatca ttataaacat tgtcgagaat atcgtcacta ctgtcaggca cacaaagtag
     7261 atgacaatat tacggaaatt gatgacatac ctgtattccc aacatcggtt tttaagttta
     7321 ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg gtttaccagt agcggcacga
     7381 atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat tgagagactc ttaggctctg
     7441 tgagttatgg catgaaatat gttggtagtt ggtttgatca tcaaatagaa ttagtcaatt
     7501 tgggaccaga tagatttaat gctcataata tttggtttaa atatgttatg agtttggtgg
     7561 aattgttata tcctacgaca tttaccgtaa cagaagaacg aatagatttt gttaaaacat
     7621 tgaatagtct tgaacgaata aaaaatcaag ggaaagatct ttgtcttatt ggttcgccat
     7681 actttattta tttactctgc cattatatga aagataaaaa aatctcattt tctggagata
     7741 aaagccttta tatcataacc ggaggcggct ggaaaagtta cgaaaaagaa tctctgaaac
     7801 gtgatgattt caatcatctt ttatttgata ctttcaatct cagtgatatt agtcagatcc
     7861 gagatatatt taatcaagtt gaactcaaca cttgtttctt tgaggatgaa atgcagcgta
     7921 aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc tgaaacgttg aaacctgtac
     7981 ctgatggaac gccggggttg atgagttata tggatgcgtc agcaaccagt tatccagcat
     8041 ttattgttac cgatgatgtc gggataatta gcagagaata tggtaagtat cccggcgtgc
     8101 tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa agggtgtgct ttaagcttaa
     8161 ccgaagcgtt tgatagttga tatccctgtc gacaagggcg aattctgcag tggatggtgg
     8221 acgatcgggt cgccggaccg ttgatccgct ccgtgctgcc ggcgggctgg tttatcgccg
     8281 ataagaccgg agctagcgag cggggtgcgc gcgggattgt cgccctgctt ggcccgaata
     8341 acaaagcaga gcgcattgtg gtgatttatc tgcgggatac cccggcgagc atggccgagc
     8401 gaaatcagca aatcgccggg atcggcgcgg cgctgatcga gcactggcaa cgctaactcg
     8461 aatcggaccg ttccaactta ccgaccagtt cggcaggtat gtatttgcgt gagcaaaagc
     8521 aacaagacta tgaagctaga atgactatta gtgaagagtt aggtcatggt agagaagatg
     8581 taacagtcaa ctacttaggc agataaaaag caatatagct atagaagaaa agaaagctat
     8641 tttacatagt agatcgactc ttcttaggga ttttatattt tttgataaat catctatttt
     8701 gctagttaaa tcatcaaatt tatcatcttg ttgtttgact aaatctaaga atctattctc
     8761 ttttttaaaa tcgttcatgc aaaccgccta tagctttctt ctttttctga aattatttgt
     8821 cttcacacca taattaaatt cccattttta taagtaaagt cttttaaaag cttgtcagtc
     8881 tcttctctag aaatgtacca aattttacct attttaggat acttttcatg aagctcttct
     8941 atttttcccc agtcctttaa tagtctacct ttagagtctc gtaaatagtt atctttgtga
     9001 caggggcctc ttttatcttt tttaatgtaa ctatatgtta ttccaacgtc actattacta
     9061 ttatccaaat cttttttagc atgccagtaa gaactttcat aacttaactc tatctttcga
     9121 cctctttgat atacaacaat aaagctatag ccagtagtaa caacctgttt tacttttgtt
     9181 aaatctatta acttcttatt tattttttta tgtttttttg aaaatttaaa tatttctata
     9241 ttcattccta cacttcctca aatccaaatg gtagcttatg attctcttct ggtttctttt
     9301 ctaatttttt tatatttgca ataaaaactc tttttctatc tttgattttt ttattgtccc
     9361 aattcctcca agtatcatca caaacccttt caatatcatg taaatgatga tgtctaaata
     9421 ttgatctgac ataatacaga tctaggtcta gttcatcact taacacaact tctctaagtc
     9481 tttcagatgc ttcgattggt atgtaatcct ctttattttt agtatctaaa agcttttgct
     9541 taaattcttc ttctgtctct gctaccttac taactgtaaa cttgatattt gtaatcttac
     9601 gaccatgttt tctgtgatga tccttgtcat cataggttac aaaaatatcc gataattgat
     9661 taatctcttc tagtgctggt aataggaact tatttttaaa atttgaatat ctgttgctgt
     9721 aacttttagg taaatcaaaa tcattaatca tatcatcgac atacaatacg caatcaacta
     9781 tattagcata ccctgcttgt tcgcctaatt tgcttttgag aagtaagtat aatctgcttg
     9841 aatacttact tttaaatgaa aatagtaact gtctttctgc tttagtaaag tactcttgta
     9901 gttgtatcat gtgtggcatt aatgaccaat gaaactcgca aattaaagca ctgcttttag
     9961 ggtctgcttc aatatatgca aaccagttag ctatcttcgt ttgttcttta ttcagccata
    10021 ctggcttaga cattattgag tgcattaatt gcttcaatct cactctgtta tgcttaaccc
    10081 ctgtagcttt ttcaagatca gataggctta tcttatacct gtgaaactct ttatcttctc
    10141 ttttaaccat tgaggcaact aagaatatta agttttgttc ttcttttgta aggctatact
    10201 ttcctgcaac aagagtatta gacatagcta tttctttgcc agcatttaca tttttaactt
    10261 ctttca
//
