LOCUS       40924_47053       14103 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant DNA excision vector pX6-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Zuo J, Niu QW, Moller SG, Chua NH.
  TITLE     Chemical-regulated, site-specific DNA excision in transgenic plants
  JOURNAL   Nat. Biotechnol. 19 (2), 157-161 (2001)
  PUBMED    11175731
REFERENCE   2  (bases 1 to 14103)
  AUTHORS   Zuo J, Chua N-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2000) Laboratory of Plant Molecular Biology, The 
            Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
REFERENCE   3  (bases 1 to 14103)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2001"; volume: "19"; issue: "2"; pages: 
            "157-161"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-DEC-2000) Laboratory of Plant Molecular Biology, The Rockefeller
            University, 1230 York Avenue, New York, NY 10021, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    complement(280..313)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             332..583
                     /label=LexA BD
                     /note="DNA-binding domain of E. coli LexA"
     CDS             599..832
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     CDS             842..1777
                     /label=ERT2
                     /note="mutated ligand-binding domain of the human estrogen 
                     receptor (Feil et al., 1997)"
     3'UTR           1797..1890
                     /label=rat glucocorticoid receptor
                     /note="rat glucocorticoid receptor"
     regulatory      1916..2211
                     /label=pea rbcS E9
                     /note="pea rbcS E9"
                     /regulatory_class="terminator"
     promoter        2368..2551
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2575..3363
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     misc_feature    3368..3739
                     /label=derived from pBI101
                     /note="derived from pBI101"
     terminator      3756..4008
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4099..4249
                     /label=LexA operator
                     /note="LexA operator"
     regulatory      4329..4387
                     /label=35S promoter
                     /note="35S promoter"
                     /regulatory_class="promoter"
     promoter        4331..4377
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     5'UTR           4390..4408
                     /label=cre
                     /note="cre"
     CDS             join(4409..4840,5011..5610)
                     /codon_start=1
                     /product="cre"
                     /label=cre
                     /protein_id="AAK08508.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          4841..5010
                     /number=5
                     /note="Arabidopsis KOR1 gene"
     3'UTR           5611..5620
                     /label=cre
                     /note="cre"
     regulatory      5624..6036
                     /label=Nos
                     /note="Nos"
                     /regulatory_class="terminator"
     terminator      5788..6040
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(6041..6074)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6101..6814
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      6830..7299
                     /label=3A
                     /note="3A"
                     /regulatory_class="terminator"
     misc_feature    7305..7505
                     /label=derived from pUC9
                     /note="derived from pUC9"
     primer_bind     complement(7331..7347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7506..7562
                     /label=derived from pBI101
                     /note="derived from pBI101"
     misc_feature    complement(7569..7593)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8121..8909
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      9156..9744
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9930..10070)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10414..10608)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10677..11741)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12178..12804)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatcccctaa taacttcgta tagcatacat
      301 tatacgaagt tatgaattaa atccgggcgg aatgaaagcg ttaacggcca ggcaacaaga
      361 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga
      421 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct
      481 ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca
      541 ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgtcgagcgc
      601 ccccccgacc gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggcgat
      661 ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc
      721 gggtccggga tttacccccc acgactccgc cccctacggc gctctggata tggccgactt
      781 cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg gggatccgtc
      841 tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa
      901 gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc
      961 tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat
     1021 gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa
     1081 gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc
     1141 ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct
     1201 actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt
     1261 ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg
     1321 agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct
     1381 gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat
     1441 cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca
     1501 gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat
     1561 ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga
     1621 gatgctggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga
     1681 gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta
     1741 ttacatcacg ggggaggcag agggtttccc tgccacagtc tgagagctcc ctggcgaatt
     1801 cccagagatg ttagctgaaa tcatcactaa tcagatacca aaatattcaa atggaaatat
     1861 caaaaagctt ctgtttcatc aaaaatgact cgacctaact gagtaagcta gcttgttcga
     1921 gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg
     1981 tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa
     2041 tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt
     2101 gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg
     2161 tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacat cccaaacaag
     2221 cttggaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac
     2281 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg
     2341 caacgattga aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac
     2401 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
     2461 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
     2521 tcatattcac tctcaatcca aataatctgc accggatctg gatcgtttcg catgattgaa
     2581 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     2641 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     2701 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     2761 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     2821 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     2881 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     2941 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3001 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3061 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat
     3121 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3181 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3241 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3301 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3361 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     3421 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     3481 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacggga
     3541 tctctgcgga acaggcggtc gaaggtgccg atatcattac gacagcaacg gccgacaagc
     3601 acaacgccac gatcctgagc gacaatatga tcgggcccgg cgtccacatc aacggcgtcg
     3661 gcggcgactg cccaggcaag accgagatgc accgcgatat cttgctgcgt tcggatattt
     3721 tcgtggagtt cccgccacag acccggatga tccccgatcg ttcaaacatt tggcaataaa
     3781 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     3841 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     3901 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     3961 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg ggaattgatc
     4021 ccccctcgac agcttgcatg ccagcttggg ctgcaggtcg aggctaaaaa actaatcgca
     4081 ttatcatccc ctcgacgtac tgtacatata accactggtt ttatatacag cagtactgta
     4141 catataacca ctggttttat atacagcagt cgacgtactg tacatataac cactggtttt
     4201 atatacagca gtactgtaca tataaccact ggttttatat acagcagtcg aggtaagatt
     4261 agatatggat atgtatatgg atatgtatat ggtggtaatg ccatgtaata tgctcgactc
     4321 taggatcttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
     4381 gctgaagcta gtcgactcta gcctcgacat gtccaattta ctgaccgtac accaaaattt
     4441 gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt
     4501 cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc
     4561 gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt
     4621 tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca
     4681 tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa
     4741 tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc
     4801 aaaacaggct ctagcgttcg aacgcactga tttcgaccag gtaagtcttc ttttccttta
     4861 ctctttacag aaatggtaat ctcagatata gtaatggata agatccaaaa atgacacttt
     4921 taaccaagat tgtacgaaga tctttttaaa ctccattttt tattttgaca tctaaattgg
     4981 atttaactcg gccttgctgt attttggcag gttcgttcac tcatggaaaa tagcgatcgc
     5041 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata
     5101 gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta
     5161 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc
     5221 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg
     5281 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc
     5341 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc
     5401 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga
     5461 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc
     5521 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca
     5581 atggtgcgcc tgctggaaga tggcgattag taagaattcg catgcattca tcaatattat
     5641 tcatgcgggg aaaggcaaga ttaatccaac tggcaaatca tccagcgtga ttggtaactt
     5701 cagttccagc gacttgattc gttttggtgc tacccacgtt ttcaataagg acgagatggt
     5761 ggagtaaaga aggagtgcgt cgaagcagat cgttcaaaca tttggcaata aagtttctta
     5821 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     5881 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     5941 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     6001 gataaattat cgcgcgcggt gtcatctatg ttactagatc ataacttcgt atagcataca
     6061 ttatacgaag ttatagatct tcgacctcga gcggggtacc atgggtaagg gagaagaact
     6121 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     6181 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     6241 ttgcactact ggaaagcttc ctgttccttg gccaacactt gtcactactc ttacttatgg
     6301 tgttcaatgc ttttcaagat acccagatca tatgaagcgg cacgacttct tcaagagcgc
     6361 catgcctgag ggatacgtgc aggaaaggac catcttcttc aaggacgacg ggaactacaa
     6421 gacacgtgct gaagtcaagt ttgagggaga cacccttgtc aacaggatcg agcttaaggg
     6481 aatcgatttc aaggaggacg gaaacatcct cggccacaag ttggaataca actacaactc
     6541 ccacaacgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     6601 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     6661 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     6721 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     6781 tgggattaca catggcatgg atgaactata caaataaact agtcgatcca ggcctcccag
     6841 ctttcgtccg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc agtttcattg
     6901 cccacacacc agaatcctac taagtttgag tattatggca ttggaaaagc tgttttcttc
     6961 tatcatttgt tctgcttgta atttactgtg ttctttcagt ttttgttttc ggacatcaaa
     7021 atgcaaatgg atggataaga gttaataaat gatatggtcc ttttgttcat tctcaaatta
     7081 ttattatctg ttgtttttac tttaatgggt tgaatttaag taagaaagga actaacagtg
     7141 tgatattaag gtgcaatgtt agacatataa aacagtcttt cacctctctt tggttatgtc
     7201 ttgaattggt ttgtttcttc acttatctgt gtaatcaagt ttactatgag tctatgatca
     7261 agtaattatg caatcaagtt aagtacagta taggcttttt gtgtcgaggg ggtaccgagt
     7321 cgaggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7381 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7441 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgggtaccga gctcgaattc
     7501 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     7561 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     7621 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     7681 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     7741 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     7801 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     7861 ctcgttcggt tcgtaagctg taatgcaagt agcgtaactg ccgtcacgca actggtccag
     7921 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttcttgtta
     7981 tgacatgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     8041 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     8101 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     8161 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8221 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8281 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8341 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8401 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8461 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8521 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8581 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8641 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8701 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8761 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     8821 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     8881 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     8941 cgccgcttcg ccggcgttaa ctcaagcgat tagatgcact aagcacataa ttgctcacag
     9001 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
     9061 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga
     9121 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9181 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9241 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9301 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9361 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9421 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9481 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9541 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9601 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9661 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9721 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9781 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9841 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9901 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     9961 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10021 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
    10081 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
    10141 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
    10201 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
    10261 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
    10321 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
    10381 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
    10441 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
    10501 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
    10561 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct
    10621 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
    10681 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
    10741 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
    10801 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
    10861 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
    10921 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
    10981 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
    11041 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
    11101 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
    11161 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
    11221 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
    11281 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
    11341 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
    11401 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
    11461 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
    11521 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
    11581 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
    11641 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
    11701 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg
    11761 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca
    11821 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
    11881 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
    11941 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
    12001 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
    12061 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
    12121 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
    12181 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
    12241 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
    12301 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
    12361 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt
    12421 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg
    12481 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc
    12541 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc
    12601 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc
    12661 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc
    12721 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg
    12781 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc
    12841 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt
    12901 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca
    12961 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg
    13021 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg
    13081 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg
    13141 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg
    13201 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa
    13261 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg
    13321 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg
    13381 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg
    13441 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca
    13501 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg
    13561 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt
    13621 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca
    13681 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg
    13741 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc
    13801 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc
    13861 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt
    13921 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
    13981 tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa
    14041 cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct
    14101 tag
//
