LOCUS       62056_22755       11947 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        268..343
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        440..725
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer;
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        727..1004
                     /label=chicken beta-actin promoter
     CDS             1251..5354
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5391..5411
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5415..5435
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5442..8177
                     /codon_start=1
                     /label=DNMT3A
                     /note="DNA methyltransferase 3A"
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     CDS             8247..8960
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    8994..9201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   9210..9350
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      9425..9880
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10162..10266
                     /label=AmpR promoter
     CDS             10267..11124
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11298..11886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
      361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
      421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
      541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
      661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
     1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
     1201 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc atggacaaga
     1261 agtattctat cggactggcc atcgggacta atagcgtcgg gtgggccgtg atcactgacg
     1321 agtacaaggt gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca
     1381 agaaaaatct gatcggagct ctcctctttg attcagggga gaccgctgaa gcaacccgcc
     1441 tcaagcggac tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag
     1501 agatattctc caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat
     1561 cattcctcgt ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg
     1621 acgaggtggc ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg
     1681 actcaaccga caaggcagac ctccggctta tctacctggc cctggcccac atgatcaagt
     1741 tcagaggcca cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac
     1801 tgttcatcca gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa
     1861 gcggagtcga tgccaaggcc attctgtcag cccggctgtc aaagagccgc agacttgaga
     1921 atcttatcgc tcagctgccg ggtgaaaaga aaaatggact gttcgggaac ctgattgctc
     1981 tttcacttgg gctgactccc aatttcaagt ctaatttcga cctggcagag gatgccaagc
     2041 tgcaactgtc caaggacacc tatgatgacg atctcgacaa cctcctggcc cagatcggtg
     2101 accaatacgc cgaccttttc cttgctgcta agaatctttc tgacgccatc ctgctgtctg
     2161 acattctccg cgtgaacact gaaatcacca aggcccctct ttcagcttca atgattaagc
     2221 ggtatgatga gcaccaccag gacctgaccc tgcttaaggc actcgtccgg cagcagcttc
     2281 cggagaagta caaggaaatc ttctttgacc agtcaaagaa tggatacgcc ggctacatcg
     2341 acggaggtgc ctcccaagag gaattttata agtttatcaa acctatcctt gagaagatgg
     2401 acggcaccga agagctcctc gtgaaactga atcgggagga tctgctgcgg aagcagcgca
     2461 ctttcgacaa tgggagcatt ccccaccaga tccatcttgg ggagcttcac gccatccttc
     2521 ggcgccaaga ggacttctac ccctttctta aggacaacag ggagaagatt gagaaaattc
     2581 tcactttccg catcccctac tacgtgggac ccctcgccag aggaaatagc cggtttgctt
     2641 ggatgaccag aaagtcagaa gaaactatca ctccctggaa cttcgaagag gtggtggaca
     2701 agggagccag cgctcagtca ttcatcgaac ggatgactaa cttcgataag aacctcccca
     2761 atgagaaggt cctgccgaaa cattccctgc tctacgagta ctttaccgtg tacaacgagc
     2821 tgaccaaggt gaaatatgtc accgaaggga tgaggaagcc cgcattcctg tcaggcgaac
     2881 aaaagaaggc aattgtggac cttctgttca agaccaatag aaaggtgacc gtgaagcagc
     2941 tgaaggagga ctatttcaag aaaattgaat gcttcgactc tgtggagatt agcggggtcg
     3001 aagatcggtt caacgcaagc ctgggtacct accatgatct gcttaagatc atcaaggaca
     3061 aggattttct ggacaatgag gagaacgagg acatccttga ggacattgtc ctgactctca
     3121 ctctgttcga ggaccgggaa atgatcgagg agaggcttaa gacctacgcc catctgttcg
     3181 acgataaagt gatgaagcaa cttaaacgga gaagatatac cggatgggga cgccttagcc
     3241 gcaaactcat caacggaatc cgggacaaac agagcggaaa gaccattctt gatttcctta
     3301 agagcgacgg attcgctaat cgcaacttca tgcaacttat ccatgatgat tccctgacct
     3361 ttaaggagga catccagaag gcccaagtgt ctggacaagg tgactcactg cacgagcata
     3421 tcgcaaatct ggctggttca cccgctatta agaagggtat tctccagacc gtgaaagtcg
     3481 tggacgagct ggtcaaggtg atgggtcgcc ataaaccaga gaacattgtc atcgagatgg
     3541 ccagggaaaa ccagactacc cagaagggac agaagaacag cagggagcgg atgaaaagaa
     3601 ttgaggaagg gattaaggag ctcgggtcac agatccttaa agagcacccg gtggaaaaca
     3661 cccagcttca gaatgagaag ctctatctgt actaccttca aaatggacgc gatatgtatg
     3721 tggaccaaga gcttgatatc aacaggctct cagactacga cgtggacgcc atcgtccctc
     3781 agagcttcct caaagacgac tcaattgaca ataaggtgct gactcgctca gacaagaacc
     3841 ggggaaagtc agataacgtg ccctcagagg aagtcgtgaa aaagatgaag aactattggc
     3901 gccagcttct gaacgcaaag ctgatcactc agcggaagtt cgacaatctc actaaggctg
     3961 agaggggcgg actgagcgaa ctggacaaag caggattcat taaacggcaa cttgtggaga
     4021 ctcggcagat tactaaacat gtcgcccaaa tccttgactc acgcatgaat accaagtacg
     4081 acgaaaacga caaacttatc cgcgaggtga aggtgattac cctgaagtcc aagctggtca
     4141 gcgatttcag aaaggacttt caattctaca aagtgcggga gatcaataac tatcatcatg
     4201 ctcatgacgc atatctgaat gccgtggtgg gaaccgccct gatcaagaag tacccaaagc
     4261 tggaaagcga gttcgtgtac ggagactaca aggtctacga cgtgcgcaag atgattgcca
     4321 aatctgagca ggagatcgga aaggccaccg caaagtactt cttctacagc aacatcatga
     4381 atttcttcaa gaccgaaatc acccttgcaa acggtgagat ccggaagagg ccgctcatcg
     4441 agactaatgg ggagactggc gaaatcgtgt gggacaaggg cagagatttc gctaccgtgc
     4501 gcaaagtgct ttctatgcct caagtgaaca tcgtgaagaa aaccgaggtg caaaccggag
     4561 gcttttctaa ggaatcaatc ctccccaagc gcaactccga caagctcatt gcaaggaaga
     4621 aggattggga ccctaagaag tacggcggat tcgattcacc aactgtggct tattctgtcc
     4681 tggtcgtggc taaggtggaa aaaggaaagt ctaagaagct caagagcgtg aaggaactgc
     4741 tgggtatcac cattatggag cgcagctcct tcgagaagaa cccaattgac tttctcgaag
     4801 ccaaaggtta caaggaagtc aagaaggacc ttatcatcaa gctcccaaag tatagcctgt
     4861 tcgaactgga gaatgggcgg aagcggatgc tcgcctccgc tggcgaactt cagaagggta
     4921 atgagctggc tctcccctcc aagtacgtga atttcctcta ccttgcaagc cattacgaga
     4981 agctgaaggg gagccccgag gacaacgagc aaaagcaact gtttgtggag cagcataagc
     5041 attatctgga cgagatcatt gagcagattt ccgagttttc taaacgcgtc attctcgctg
     5101 atgccaacct cgataaagtc cttagcgcat acaataagca cagagacaaa ccaattcggg
     5161 agcaggctga gaatatcatc cacctgttca ccctcaccaa tcttggtgcc cctgccgcat
     5221 tcaagtactt cgacaccacc atcgaccgga aacgctatac ctccaccaaa gaagtgctgg
     5281 acgccaccct catccaccag agcatcaccg gactttacga aactcggatt gacctctcac
     5341 agctcggagg ggatgaggga gctgatccaa aaaagaagag aaaggtagac ccaaagaaaa
     5401 aacgaaaagt agatccgaaa aagaagagga aggtgggatc catgcccgcc atgccctcca
     5461 gcggccccgg ggacaccagc agctctgctg cggagcggga ggaggaccga aaggacggag
     5521 aggagcagga ggagccgcgt ggcaaggagg agcgccaaga gcccagcacc acggcacgga
     5581 aggtggggcg gcctgggagg aagcgcaagc accccccggt ggaaagcggt gacacgccaa
     5641 aggaccctgc ggtgatctcc aagtccccat ccatggccca ggactcaggc gcctcagagc
     5701 tattacccaa tggggacttg gagaagcgga gtgagcccca gccagaggag gggagccctg
     5761 ctggggggca gaagggcggg gccccagcag agggagaggg tgcagctgag accctgcctg
     5821 aagcctcaag agcagtggaa aatggctgct gcacccccaa ggagggccga ggagcccctg
     5881 cagaagcggg caaagaacag aaggagacca acatcgaatc catgaaaatg gagggctccc
     5941 ggggccggct gcggggtggc ttgggctggg agtccagcct ccgtcagcgg cccatgccga
     6001 ggctcacctt ccaggcgggg gacccctact acatcagcaa gcgcaagcgg gacgagtggc
     6061 tggcacgctg gaaaagggag gctgagaaga aagccaaggt cattgcagga atgaatgctg
     6121 tggaagaaaa ccaggggccc ggggagtctc agaaggtgga ggaggccagc cctcctgctg
     6181 tgcagcagcc cactgacccc gcatccccca ctgtggctac cacgcctgag cccgtggggt
     6241 ccgatgctgg ggacaagaat gccaccaaag caggcgatga cgagccagag tacgaggacg
     6301 gccggggctt tggcattggg gagctggtgt gggggaaact gcggggcttc tcctggtggc
     6361 caggccgcat tgtgtcttgg tggatgacgg gccggagccg agcagctgaa ggcacccgct
     6421 gggtcatgtg gttcggagac ggcaaattct cagtggtgtg tgttgagaag ctgatgccgc
     6481 tgagctcgtt ttgcagtgcg ttccaccagg ccacgtacaa caagcagccc atgtaccgca
     6541 aagccatcta cgaggtcctg caggtggcca gcagccgcgc ggggaagctg ttcccggtgt
     6601 gccacgacag cgatgagagt gacactgcca aggccgtgga ggtgcagaac aagcccatga
     6661 ttgaatgggc cctggggggc ttccagcctt ctggccctaa gggcctggag ccaccagaag
     6721 aagagaagaa tccctacaaa gaagtgtaca cggacatgtg ggtggaacct gaggcagctg
     6781 cctacgcacc acctccacca gccaaaaagc cccggaagag cacagcggag aagcccaagg
     6841 tcaaggagat tattgatgag cgcacaagag agcggctggt gtacgaggtg cggcagaagt
     6901 gccggaacat tgaggacatc tgcatctcct gtgggagcct caatgttacc ctggaacacc
     6961 ccctcttcgt tggaggaatg tgccaaaact gcaagaactg ctttctggag tgtgcgtacc
     7021 agtacgacga cgacggctac cagtcctact gcaccatctg ctgtgggggc cgtgaggtgc
     7081 tcatgtgcgg aaacaacaac tgctgcaggt gcttttgcgt ggagtgtgtg gacctcttgg
     7141 tggggccggg ggctgcccag gcagccatta aggaagaccc ctggaactgc tacatgtgcg
     7201 ggcacaaggg tacctacggg ctgctgcggc ggcgagagga ctggccctcc cggctccaga
     7261 tgttcttcgc taataaccac gaccaggaat ttgaccctcc aaaggtttac ccacctgtcc
     7321 cagctgagaa gaggaagccc atccgggtgc tgtctctctt tgatggaatc gctacagggc
     7381 tcctggtgct gaaggacttg ggcattcagg tggaccgcta cattgcctcg gaggtgtgtg
     7441 aggactccat cacggtgggc atggtgcggc accaggggaa gatcatgtac gtcggggacg
     7501 tccgcagcgt cacacagaag catatccagg agtggggccc attcgatctg gtgattgggg
     7561 gcagtccctg caatgacctc tccatcgtca accctgctcg caagggcctc tacgagggca
     7621 ctggccggct cttctttgag ttctaccgcc tcctgcatga tgcgcggccc aaggagggag
     7681 atgatcgccc cttcttctgg ctctttgaga atgtggtggc catgggcgtt agtgacaaga
     7741 gggacatctc gcgatttctc gagtccaacc ctgtgatgat tgatgccaaa gaagtgtcag
     7801 ctgcacacag ggcccgctac ttctggggta accttcccgg tatgaacagg ccgttggcat
     7861 ccactgtgaa tgataagctg gagctgcagg agtgtctgga gcatggcagg atagccaagt
     7921 tcagcaaagt gaggaccatt actacgaggt caaactccat aaagcagggc aaagaccagc
     7981 attttcctgt cttcatgaat gagaaagagg acatcttatg gtgcactgaa atggaaaggg
     8041 tatttggttt cccagtccac tatactgacg tctccaacat gagccgcttg gcgaggcaga
     8101 gactgctggg ccggtcatgg agcgtgccag tcatccgcca cctcttcgct ccgctgaagg
     8161 agtattttgc gtgtgtggaa ttcggcagtg gagagggcag aggaagtctg ctaacatgcg
     8221 gtgacgtcga ggagaatcct ggcccagtga gcaagggcga ggagctgttc accggggtgg
     8281 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     8341 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     8401 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     8461 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     8521 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     8581 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     8641 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     8701 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     8761 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     8821 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     8881 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     8941 gcatggacga gctgtacaag gaattctaac tagagctcgc tgatcagcct cgactgtgcc
     9001 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     9061 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     9121 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     9181 gaatagcagg catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc
     9241 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     9301 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     9361 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     9421 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     9661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     9781 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9841 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     9901 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     9961 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    10021 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
    10081 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    10141 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10201 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10261 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10321 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    10381 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    10441 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    10501 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10561 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10621 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10681 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10741 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10801 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10861 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10921 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10981 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    11041 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    11101 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    11161 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11221 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11281 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11341 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11401 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    11461 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    11521 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11581 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11641 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11701 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11761 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11821 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11881 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11941 cacatgt
//
