LOCUS       62056_22750       11950 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        271..346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        443..728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer;
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        730..1007
                     /label=chicken beta-actin promoter
     CDS             1254..5357
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5394..5414
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5418..5438
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5445..8180
                     /codon_start=1
                     /label=DNMT3A
                     /note="DNA methyltransferase 3A"
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     CDS             8250..8963
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    8997..9204
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   9213..9353
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      9428..9883
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10165..10269
                     /label=AmpR promoter
     CDS             10270..11127
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11301..11889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg cgcttccgcg gcccgttcaa gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
      361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
      421 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
      481 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
      541 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      601 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
      661 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      721 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
      781 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
      841 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
      901 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
      961 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga
     1021 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1081 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1141 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat
     1201 tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc accatggaca
     1261 agaagtattc tatcggactg gccatcggga ctaatagcgt cgggtgggcc gtgatcactg
     1321 acgagtacaa ggtgccctct aagaagttca aggtgctcgg gaacaccgac cggcattcca
     1381 tcaagaaaaa tctgatcgga gctctcctct ttgattcagg ggagaccgct gaagcaaccc
     1441 gcctcaagcg gactgctaga cggcggtaca ccaggaggaa gaaccggatt tgttaccttc
     1501 aagagatatt ctccaacgaa atggcaaagg tcgacgacag cttcttccat aggctggaag
     1561 aatcattcct cgtggaagag gataagaagc atgaacggca tcccatcttc ggtaatatcg
     1621 tcgacgaggt ggcctatcac gagaaatacc caaccatcta ccatcttcgc aaaaagctgg
     1681 tggactcaac cgacaaggca gacctccggc ttatctacct ggccctggcc cacatgatca
     1741 agttcagagg ccacttcctg atcgagggcg acctcaatcc tgacaatagc gatgtggata
     1801 aactgttcat ccagctggtg cagacttaca accagctctt tgaagagaac cccatcaatg
     1861 caagcggagt cgatgccaag gccattctgt cagcccggct gtcaaagagc cgcagacttg
     1921 agaatcttat cgctcagctg ccgggtgaaa agaaaaatgg actgttcggg aacctgattg
     1981 ctctttcact tgggctgact cccaatttca agtctaattt cgacctggca gaggatgcca
     2041 agctgcaact gtccaaggac acctatgatg acgatctcga caacctcctg gcccagatcg
     2101 gtgaccaata cgccgacctt ttccttgctg ctaagaatct ttctgacgcc atcctgctgt
     2161 ctgacattct ccgcgtgaac actgaaatca ccaaggcccc tctttcagct tcaatgatta
     2221 agcggtatga tgagcaccac caggacctga ccctgcttaa ggcactcgtc cggcagcagc
     2281 ttccggagaa gtacaaggaa atcttctttg accagtcaaa gaatggatac gccggctaca
     2341 tcgacggagg tgcctcccaa gaggaatttt ataagtttat caaacctatc cttgagaaga
     2401 tggacggcac cgaagagctc ctcgtgaaac tgaatcggga ggatctgctg cggaagcagc
     2461 gcactttcga caatgggagc attccccacc agatccatct tggggagctt cacgccatcc
     2521 ttcggcgcca agaggacttc tacccctttc ttaaggacaa cagggagaag attgagaaaa
     2581 ttctcacttt ccgcatcccc tactacgtgg gacccctcgc cagaggaaat agccggtttg
     2641 cttggatgac cagaaagtca gaagaaacta tcactccctg gaacttcgaa gaggtggtgg
     2701 acaagggagc cagcgctcag tcattcatcg aacggatgac taacttcgat aagaacctcc
     2761 ccaatgagaa ggtcctgccg aaacattccc tgctctacga gtactttacc gtgtacaacg
     2821 agctgaccaa ggtgaaatat gtcaccgaag ggatgaggaa gcccgcattc ctgtcaggcg
     2881 aacaaaagaa ggcaattgtg gaccttctgt tcaagaccaa tagaaaggtg accgtgaagc
     2941 agctgaagga ggactatttc aagaaaattg aatgcttcga ctctgtggag attagcgggg
     3001 tcgaagatcg gttcaacgca agcctgggta cctaccatga tctgcttaag atcatcaagg
     3061 acaaggattt tctggacaat gaggagaacg aggacatcct tgaggacatt gtcctgactc
     3121 tcactctgtt cgaggaccgg gaaatgatcg aggagaggct taagacctac gcccatctgt
     3181 tcgacgataa agtgatgaag caacttaaac ggagaagata taccggatgg ggacgcctta
     3241 gccgcaaact catcaacgga atccgggaca aacagagcgg aaagaccatt cttgatttcc
     3301 ttaagagcga cggattcgct aatcgcaact tcatgcaact tatccatgat gattccctga
     3361 cctttaagga ggacatccag aaggcccaag tgtctggaca aggtgactca ctgcacgagc
     3421 atatcgcaaa tctggctggt tcacccgcta ttaagaaggg tattctccag accgtgaaag
     3481 tcgtggacga gctggtcaag gtgatgggtc gccataaacc agagaacatt gtcatcgaga
     3541 tggccaggga aaaccagact acccagaagg gacagaagaa cagcagggag cggatgaaaa
     3601 gaattgagga agggattaag gagctcgggt cacagatcct taaagagcac ccggtggaaa
     3661 acacccagct tcagaatgag aagctctatc tgtactacct tcaaaatgga cgcgatatgt
     3721 atgtggacca agagcttgat atcaacaggc tctcagacta cgacgtggac gccatcgtcc
     3781 ctcagagctt cctcaaagac gactcaattg acaataaggt gctgactcgc tcagacaaga
     3841 accggggaaa gtcagataac gtgccctcag aggaagtcgt gaaaaagatg aagaactatt
     3901 ggcgccagct tctgaacgca aagctgatca ctcagcggaa gttcgacaat ctcactaagg
     3961 ctgagagggg cggactgagc gaactggaca aagcaggatt cattaaacgg caacttgtgg
     4021 agactcggca gattactaaa catgtcgccc aaatccttga ctcacgcatg aataccaagt
     4081 acgacgaaaa cgacaaactt atccgcgagg tgaaggtgat taccctgaag tccaagctgg
     4141 tcagcgattt cagaaaggac tttcaattct acaaagtgcg ggagatcaat aactatcatc
     4201 atgctcatga cgcatatctg aatgccgtgg tgggaaccgc cctgatcaag aagtacccaa
     4261 agctggaaag cgagttcgtg tacggagact acaaggtcta cgacgtgcgc aagatgattg
     4321 ccaaatctga gcaggagatc ggaaaggcca ccgcaaagta cttcttctac agcaacatca
     4381 tgaatttctt caagaccgaa atcacccttg caaacggtga gatccggaag aggccgctca
     4441 tcgagactaa tggggagact ggcgaaatcg tgtgggacaa gggcagagat ttcgctaccg
     4501 tgcgcaaagt gctttctatg cctcaagtga acatcgtgaa gaaaaccgag gtgcaaaccg
     4561 gaggcttttc taaggaatca atcctcccca agcgcaactc cgacaagctc attgcaagga
     4621 agaaggattg ggaccctaag aagtacggcg gattcgattc accaactgtg gcttattctg
     4681 tcctggtcgt ggctaaggtg gaaaaaggaa agtctaagaa gctcaagagc gtgaaggaac
     4741 tgctgggtat caccattatg gagcgcagct ccttcgagaa gaacccaatt gactttctcg
     4801 aagccaaagg ttacaaggaa gtcaagaagg accttatcat caagctccca aagtatagcc
     4861 tgttcgaact ggagaatggg cggaagcgga tgctcgcctc cgctggcgaa cttcagaagg
     4921 gtaatgagct ggctctcccc tccaagtacg tgaatttcct ctaccttgca agccattacg
     4981 agaagctgaa ggggagcccc gaggacaacg agcaaaagca actgtttgtg gagcagcata
     5041 agcattatct ggacgagatc attgagcaga tttccgagtt ttctaaacgc gtcattctcg
     5101 ctgatgccaa cctcgataaa gtccttagcg catacaataa gcacagagac aaaccaattc
     5161 gggagcaggc tgagaatatc atccacctgt tcaccctcac caatcttggt gcccctgccg
     5221 cattcaagta cttcgacacc accatcgacc ggaaacgcta tacctccacc aaagaagtgc
     5281 tggacgccac cctcatccac cagagcatca ccggacttta cgaaactcgg attgacctct
     5341 cacagctcgg aggggatgag ggagctgatc caaaaaagaa gagaaaggta gacccaaaga
     5401 aaaaacgaaa agtagatccg aaaaagaaga ggaaggtggg atccatgccc gccatgccct
     5461 ccagcggccc cggggacacc agcagctctg ctgcggagcg ggaggaggac cgaaaggacg
     5521 gagaggagca ggaggagccg cgtggcaagg aggagcgcca agagcccagc accacggcac
     5581 ggaaggtggg gcggcctggg aggaagcgca agcacccccc ggtggaaagc ggtgacacgc
     5641 caaaggaccc tgcggtgatc tccaagtccc catccatggc ccaggactca ggcgcctcag
     5701 agctattacc caatggggac ttggagaagc ggagtgagcc ccagccagag gaggggagcc
     5761 ctgctggggg gcagaagggc ggggccccag cagagggaga gggtgcagct gagaccctgc
     5821 ctgaagcctc aagagcagtg gaaaatggct gctgcacccc caaggagggc cgaggagccc
     5881 ctgcagaagc gggcaaagaa cagaaggaga ccaacatcga atccatgaaa atggagggct
     5941 cccggggccg gctgcggggt ggcttgggct gggagtccag cctccgtcag cggcccatgc
     6001 cgaggctcac cttccaggcg ggggacccct actacatcag caagcgcaag cgggacgagt
     6061 ggctggcacg ctggaaaagg gaggctgaga agaaagccaa ggtcattgca ggaatgaatg
     6121 ctgtggaaga aaaccagggg cccggggagt ctcagaaggt ggaggaggcc agccctcctg
     6181 ctgtgcagca gcccactgac cccgcatccc ccactgtggc taccacgcct gagcccgtgg
     6241 ggtccgatgc tggggacaag aatgccacca aagcaggcga tgacgagcca gagtacgagg
     6301 acggccgggg ctttggcatt ggggagctgg tgtgggggaa actgcggggc ttctcctggt
     6361 ggccaggccg cattgtgtct tggtggatga cgggccggag ccgagcagct gaaggcaccc
     6421 gctgggtcat gtggttcgga gacggcaaat tctcagtggt gtgtgttgag aagctgatgc
     6481 cgctgagctc gttttgcagt gcgttccacc aggccacgta caacaagcag cccatgtacc
     6541 gcaaagccat ctacgaggtc ctgcaggtgg ccagcagccg cgcggggaag ctgttcccgg
     6601 tgtgccacga cagcgatgag agtgacactg ccaaggccgt ggaggtgcag aacaagccca
     6661 tgattgaatg ggccctgggg ggcttccagc cttctggccc taagggcctg gagccaccag
     6721 aagaagagaa gaatccctac aaagaagtgt acacggacat gtgggtggaa cctgaggcag
     6781 ctgcctacgc accacctcca ccagccaaaa agccccggaa gagcacagcg gagaagccca
     6841 aggtcaagga gattattgat gagcgcacaa gagagcggct ggtgtacgag gtgcggcaga
     6901 agtgccggaa cattgaggac atctgcatct cctgtgggag cctcaatgtt accctggaac
     6961 accccctctt cgttggagga atgtgccaaa actgcaagaa ctgctttctg gagtgtgcgt
     7021 accagtacga cgacgacggc taccagtcct actgcaccat ctgctgtggg ggccgtgagg
     7081 tgctcatgtg cggaaacaac aactgctgca ggtgcttttg cgtggagtgt gtggacctct
     7141 tggtggggcc gggggctgcc caggcagcca ttaaggaaga cccctggaac tgctacatgt
     7201 gcgggcacaa gggtacctac gggctgctgc ggcggcgaga ggactggccc tcccggctcc
     7261 agatgttctt cgctaataac cacgaccagg aatttgaccc tccaaaggtt tacccacctg
     7321 tcccagctga gaagaggaag cccatccggg tgctgtctct ctttgatgga atcgctacag
     7381 ggctcctggt gctgaaggac ttgggcattc aggtggaccg ctacattgcc tcggaggtgt
     7441 gtgaggactc catcacggtg ggcatggtgc ggcaccaggg gaagatcatg tacgtcgggg
     7501 acgtccgcag cgtcacacag aagcatatcc aggagtgggg cccattcgat ctggtgattg
     7561 ggggcagtcc ctgcaatgac ctctccatcg tcaaccctgc tcgcaagggc ctctacgagg
     7621 gcactggccg gctcttcttt gagttctacc gcctcctgca tgatgcgcgg cccaaggagg
     7681 gagatgatcg ccccttcttc tggctctttg agaatgtggt ggccatgggc gttagtgaca
     7741 agagggacat ctcgcgattt ctcgagtcca accctgtgat gattgatgcc aaagaagtgt
     7801 cagctgcaca cagggcccgc tacttctggg gtaaccttcc cggtatgaac aggccgttgg
     7861 catccactgt gaatgataag ctggagctgc aggagtgtct ggagcatggc aggatagcca
     7921 agttcagcaa agtgaggacc attactacga ggtcaaactc cataaagcag ggcaaagacc
     7981 agcattttcc tgtcttcatg aatgagaaag aggacatctt atggtgcact gaaatggaaa
     8041 gggtatttgg tttcccagtc cactatactg acgtctccaa catgagccgc ttggcgaggc
     8101 agagactgct gggccggtca tggagcgtgc cagtcatccg ccacctcttc gctccgctga
     8161 aggagtattt tgcgtgtgtg gaattcggca gtggagaggg cagaggaagt ctgctaacat
     8221 gcggtgacgt cgaggagaat cctggcccag tgagcaaggg cgaggagctg ttcaccgggg
     8281 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     8341 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     8401 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     8461 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     8521 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     8581 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     8641 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     8701 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     8761 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     8821 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     8881 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     8941 tcggcatgga cgagctgtac aaggaattct aactagagct cgctgatcag cctcgactgt
     9001 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     9061 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     9121 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     9181 agagaatagc aggcatgctg gggagcggcc gcaggaaccc ctagtgatgg agttggccac
     9241 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     9301 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     9361 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     9421 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     9661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9721 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     9781 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9841 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     9901 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     9961 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    10021 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    10081 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    10141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    10201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    10261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    10321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    10381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    10441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    10501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    10561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    10621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    10681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    10741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    10801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    10861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    10921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    10981 cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    11041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    11101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    11161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    11221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    11281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    11341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    11401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    11461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    11521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    11581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    11641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    11701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    11761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    11821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    11881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    11941 gctcacatgt
//
