LOCUS       V002062                 5008 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002062
VERSION     V002062
KEYWORDS    bata-lactamase (bla); catechol-2,3-dioxygenase (xylE); gentamy
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Schweizer HP, Hoang TT.
  TITLE     An improved system for gene replacement and xylE fusion analysis in
            Pseudomonas aeruginosa
  JOURNAL   Gene 158 (1), 15-22 (1995)
   PUBMED   7789804
REFERENCE   3  (bases 1 to 5008)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-NOV-1994) Herbert P. Schweizer, Microbiology "
            SGRef: number: 2; type: "Journal Article"; journalName: "Gene";
            date: "1995"; volume: "158"; issue: "1"; pages: "15-22"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             665..1585
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     promoter        1704..1732
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             1921..2451
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     misc_feature    2718..2774
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2787..2803)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2811..2827)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2835..2865)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2880..2901)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      complement(2984..3011)
                     /label="T2"
                     /note="T2"
                     /regulatory_class="terminator"
     regulatory      complement(3018..3143)
                     /label="T1"
                     /note="T1"
                     /regulatory_class="terminator"
     rep_origin      complement(3189..3777)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3951..4808)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4809..4913)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattcctccg cgagagcacc gcgatcaagg
      481 actacgattc ccgccgatga ccgtgatcgg cgaactggac aagcagaccg gcgaccttct
      541 cgaggagctt ttttttcctg gccctggcct gtgaagtgtt tccggaaacc gacttgacca
      601 tcgagtactt tcgccacgtt ggcggaaaca aacctgacaa catgaactat gaagaggtga
      661 cgtcatgaac aaaggtgtaa tgcgaccggg ccatgtgcag ctgcgtgtac tggacatgag
      721 caaggccctg gaacactacg tcgagttgct gggcctgatc gagatggacc gtgacgacca
      781 gggccgtgtc tatctgaagg cttggaccga agtggataag ttttccctgg tgctacgcga
      841 ggctgacgag ccgggcatgg attttatggg tttcaaggtt gtggatgagg atgctctccg
      901 gcaactggag cgggatctga tggcatatgg ctgtgccgtt gagcagctac ccgcaggtga
      961 actgaacagt tgtggccggc gcgtgcgctt ccaggccccc tccgggcatc acttcgagtt
     1021 gtatgcagac aaggaatata ctggaaagtg gggtttgaat gacgtcaatc ccgaggcatg
     1081 gccgcgcgat ctgaaaggta tggcggctgt gcgtttcgac cacgccctca tgtatggcga
     1141 cgaattgccg gcgacctatg acctgttcac caaggtgctc ggtttctatc tggccgaaca
     1201 ggtgctggac gaaaatggca cgcgcgtcgc ccagtttctc agtctgtcga ccaaggccca
     1261 cgacgtggcc ttcattcacc atccggaaaa aggccgcctc catcatgtgt ccttccacct
     1321 cgaaacctgg gaagacttgc ttcgcgccgc cgacctgatc tccatgaccg acacatctat
     1381 cgatatcggc ccaacccgcc acggcctcac tcacggcaag accatctact tcttcgaccc
     1441 gtccggtaac cgcaacgaag tgttctgcgg gggagattac aactacccgg accacaaacc
     1501 ggtgacctgg accaccgacc agctgggcaa ggcgatcttt taccacgacc gcattctcaa
     1561 cgaacgattc atgaccgtgc tgacctgatg gtccggtacg acttattgca gagattgtgc
     1621 agatgaaaga aatcaagcat ttcattagcg gtgaattagt cggttcggcc agcggcaagc
     1681 tgttcgacaa tgtcagcccc gaattgacat aagcctgttc ggttcgtaaa ctgtaatgca
     1741 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1801 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg
     1861 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1921 atgttacgca gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaggt
     1981 ggctcaagta tgggcatcat tcgcacatgt aggctcggcc ctgaccaagt caaatccatg
     2041 cgggctgctc ttgatctttt cggtcgtgag ttcggagacg tagccaccta ctcccaacat
     2101 cagccggact ccgattacct cgggaacttg ctccgtagta agacattcat cgcgcttgct
     2161 gccttcgacc aagaagcggt tgttggcgct ctcgcggctt acgttctgcc caggtttgag
     2221 cagccgcgta gtgagatcta tatctatgat ctcgcagtct ccggcgagca ccggaggcag
     2281 ggcattgcca ccgcgctcat caatctcctc aagcatgagg ccaacgcgct tggtgcttat
     2341 gtgatctacg tgcaagcaga ttacggtgac gatcccgcag tggctctcta tacaaagttg
     2401 ggcatacggg aagaagtgat gcactttgat atcgacccaa gtaccgccac ctaacaattc
     2461 gttcaagccg agatcggctt cccggccgcg gagttgttcg gtaaattgtc acaacgccgc
     2521 ggccaattcg gggcaacggc caggtgatcg gccgcgtcca cgaggccggc cgcgccgagg
     2581 tcgacgccgc ggtcagggcg gcacgcgctg cgctgaaggg accctggggg aagatgacgg
     2641 tggccgagcg cgctgagatt ctgcatcgcg tggccgatgg catcacggcg cgcttcggcg
     2701 agtttctcga cggatcggaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
     2761 aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2821 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2881 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2941 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3001 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3061 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3601 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4261 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4321 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4381 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4441 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4501 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4561 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4621 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4681 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4741 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4801 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4861 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4921 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4981 aataggcgta tcacgaggcc ctttcgtc
//
