LOCUS       40924_46923        5187 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pX-DR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 5187)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4..256)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             764..1066
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    complement(1125..1181)
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(1182..1856)
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     promoter        complement(1914..1960)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     rep_origin      complement(2586..3041)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3068..3172
                     /label=AmpR promoter
     CDS             3173..4030
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4204..4792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(5145..5169)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
       61 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
      121 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
      181 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
      241 aaatgtttga acgatcgggg aaattcgagc tggtcacctg taattcacac aacaacaaca
      301 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      361 aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc ggtggatccc
      421 caatacttgt atggggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
      481 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
      541 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
      601 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
      661 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
      721 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
      781 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
      841 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
      901 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
      961 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     1021 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     1081 ccttatacac agccagtctg caggtcgacc catacaagta ttggggatcc cgggcccgcg
     1141 gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc tcaggaacag gtggtggcgg
     1201 ccctcggtgc gctcgtactg ctccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
     1261 agcttggcgt ccacgtagta gtagccgggc agctgcacgg gcttcttggc catgtagatg
     1321 gacttgaact ccaccaggta gtggccgccg tccttcagct tcagggcctt gtgggtctcg
     1381 cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca gcccatggtc
     1441 ttcttctgca tcacggggcc gtcggagggg aagttcacgc cgatgaactt caccttgtag
     1501 atgaagcagc cgtcctgcag ggaggagtcc tgggtcacgg tcgccacgcc gccgtcctcg
     1561 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttctt gtagtcgggg
     1621 atgtcggcgg ggtgcttcac gtacaccttg gagccgtact ggaactgggg ggacaggatg
     1681 tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttcacggt gttgtggccc
     1741 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggtgccc
     1801 tccatgcgca ccttgaagcg catgaactcg gtgatgacgt tctcggagga ggccatggtg
     1861 gcgaccggta gcgctagagg atcttgtaga gagagactgg tgatttcagc gtgtcctctc
     1921 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     1981 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     2041 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     2101 ggcagaggca tcttgaatga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     2161 accttccttt tctactgtcc ttttgatgaa gtgacagata ggatcggaat taattccccg
     2221 gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag
     2281 ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactca gccgcggtgg
     2341 agctcgaatt tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag
     2401 ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     2461 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtctggc
     2521 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     2581 aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2641 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2701 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2761 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2821 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2881 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2941 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3001 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     3061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4321 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4441 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4501 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4561 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4621 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4681 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4741 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4801 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4861 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4921 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4981 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagac tttgatcccg
     5041 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
     5101 ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
     5161 atcctgtcaa acactgatag tttaatt
//
