LOCUS       40924_46918        5235 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pX-DG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 5235)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4..256)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(478..780)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    complement(1125..1190)
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(1191..1904)
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTMGYGVLCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(1962..2008)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     rep_origin      complement(2634..3089)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3116..3220
                     /label=AmpR promoter
     CDS             3221..4078
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4252..4840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(5193..5217)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
       61 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
      121 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
      181 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
      241 aaatgtttga acgatcgggg aaattcgagc tggtcacctg taattcacac aacaacaaca
      301 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      361 aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc ggtggatccc
      421 caatacttgt atgggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat
      481 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
      541 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
      601 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag
      661 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
      721 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
      781 ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct
      841 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
      901 tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg
      961 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac
     1021 atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac
     1081 gcatactgtt atctggcttt tagtaagccc catacaagta ttggggatcc cgggcccgcg
     1141 gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc tgagtccgga cttgtatagt
     1201 tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct
     1261 ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt
     1321 aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
     1381 cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg
     1441 tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg
     1501 tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct
     1561 tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt
     1621 tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatct
     1681 gggtatcttg aaaagcatag aacaccataa cccatagtag tgacaagtgt tggccatgga
     1741 acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca
     1801 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca
     1861 agaattggga caactccagt gaaaagttct tctcctttac ccatggtggc gaccggtagc
     1921 gctagaggat cttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga
     1981 acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac
     2041 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
     2101 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
     2161 ttgaatgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
     2221 tactgtcctt ttgatgaagt gacagatagg atcggaatta attccccgga tcatgagcgg
     2281 agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt
     2341 tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcggtggag ctcgaatttc
     2401 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     2461 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     2521 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtctggcgt aatagcgaag
     2581 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt
     2641 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2701 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2761 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2821 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     2881 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2941 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3001 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3061 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3121 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3181 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3241 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3301 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3361 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3421 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3481 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3541 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3601 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3661 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3721 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3781 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3841 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3901 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3961 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4021 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4081 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4141 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4201 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4261 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4321 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4381 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4441 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4501 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4561 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4621 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4681 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4741 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4801 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4861 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4921 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4981 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5041 gcctctcccc gcgcgttggc cgattcatta atgcagactt tgatcccgag gggaaccctg
     5101 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     5161 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     5221 actgatagtt taatt
//
