LOCUS       40924_46908        5575 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pWY109, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Rozwadowski K, Yang W, Kagale S.
  TITLE     Homologous recombination-mediated cloning and manipulation of 
            genomic DNA regions using Gateway and recombineering systems
  JOURNAL   BMC Biotechnol. 8, 88 (2008)
  PUBMED    19014699
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Rozwadowski KL, Yang W, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2008) Molecular Genetics, Agriculture and 
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 
            0X2, Canada
REFERENCE   3  (bases 1 to 5575)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 8, 88 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2008) Molecular Genetics, Agriculture and Agri-Food Canada, 
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(106..130)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     regulatory      231..733
                     /label=tCUP2 promoter
                     /note="tCUP2 promoter"
                     /regulatory_class="promoter"
     CDS             830..1282
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      1404..1656
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1673..1689)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1770..1794)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      1872..2503
                     /label=oriV
                     /note="incP origin of replication"
     CDS             3346..4020
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /protein_id="ACL35315.1"
                     /translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI
                     GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY
                     QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT
                     MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK
                     KL"
     gene            3346..4020
                     /gene="aad9"
                     /label=aad9
     CDS             4381..5526
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
ORIGIN
        1 tgagatctcc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
       61 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
      121 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gagctccacc
      181 gcggtggcgg ccgctctaga actagccaat tggggcccaa cgttctcgag gggatcttct
      241 gcaagcatct ctatttcctg aaggtctaac ctcgaagatt taagatttaa ttacgtttat
      301 aattacaaaa ttgattctag tatctttaat ttaatgctta tacattatta attaatttag
      361 tactttcaat ttgttttcag aaattatttt actatttttt ataaaataaa agggagaaaa
      421 tggctattta aatactagcc tattttattt caattttagc ttaaaatcag ccccaattag
      481 ccccaatttc aaattcaaat ggtccagccc aattcctaaa taacccaccc ctaacccgcc
      541 cggtttcccc ttttgatcca tgcagtcaac gcccagaatt tccctatata attttttaat
      601 tcccaaacac ccctaactct atcccatttc tcaccaaccg ccacatagat ctatcctctt
      661 atctctcaaa ctctctcgaa ccttccccta accctagcag cctctcatca tcctcacctc
      721 aaaacccacc ggatctagag catcgaattc ctgcagccca gcttgataaa ggccggccat
      781 gtctccggag aggagaccag ttgagattag gccagctaca gcagctgata tggccgctgt
      841 ttgtgacatc gttaaccatt acattgagac ttctacagtg aactttagga cagagccaca
      901 aacaccacaa gagtggattg atgatcttga gaggttgcaa gatagatacc cttggttggt
      961 tgctgaggtt gagggtgttg tggctggtat tgcttacgct ggaccttgga aggctaggaa
     1021 cgcttacgat tggacagttg agagtactgt ttacgtgtca cataggcatc aaaggttggg
     1081 cctcggatct acattgtaca cacatttgct taagtctatg gaggcgcaag gttttaagtc
     1141 tgtggttgct gttattggcc ttccaaacga tccatctgtt aggttgcatg aggctttggg
     1201 atacacagcc aggggtacat tgcgcgcagc tggatacaag catggtggat ggcatgatgt
     1261 tggtttttgg caaagggatt ttgagttgcc agctcctcca aggccagtta gaccagttac
     1321 ccagatctga ggcgcgccga attcctgcag cccgggggat ccactagtga tatccctgtg
     1381 tgaaattgtt atccgctacg cgtgatcgtt caaacatttg gcaataaagt ttcttaagat
     1441 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     1501 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     1561 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     1621 aattatcgcg cgcggtgtca tctatgttac tagatcccat gatcaagctt atcgataccg
     1681 tcgacctcga gggggggccc ggtaccaaaa ccaccccagt acattaaaaa cgtccgcaat
     1741 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     1801 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     1861 tcagtccact agacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
     1921 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
     1981 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     2041 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     2101 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
     2161 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     2221 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     2281 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     2341 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     2401 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     2461 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctctcgagt tggcagcatc
     2521 acccataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     2581 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     2641 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     2701 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     2761 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     2821 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     2881 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     2941 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagctag ccgttcgtga
     3001 atacatgtta taataactat aactaataac gtaacgtgac tggcaagaga tatttttaaa
     3061 acaatgaata ggtttacact tactttagtt ttatggaaat gaaagatcat atcatatata
     3121 atctagaata aaattaacta aaataattat tatctagata aaaaatttag aagccaatga
     3181 aatctataaa taaactaaat taagtttatt taattaacaa ctatggatat aaaataggta
     3241 ctaatcaaaa tagtgaggag gatatatttg aatacatacg aacaaattaa taaagtgaaa
     3301 aaaatacttc ggaaacattt aaaaaataac cttattggta cttacatgtt tggatcagga
     3361 gttgagagtg gactaaaacc aaatagtgat cttgactttt tagtcgtcgt atctgaacca
     3421 ttgacagatc aaagtaaaga aatacttata caaaaaatta gacctatttc aaaaaaaata
     3481 ggagataaaa gcaacttacg atatattgaa ttaacaatta ttattcagca agaaatggta
     3541 ccgtggaatc atcctcccaa acaagaattt atttatggag aatggttaca agagctttat
     3601 gaacaaggat acattcctca gaaggaatta aattcagatt taaccataat gctttaccaa
     3661 gcaaaacgaa aaaataaaag aatatacgga aattatgact tagaggaatt actacctgat
     3721 attccatttt ctgatgtgag aagagccatt atggattcgt cagaggaatt aatagataat
     3781 tatcaggatg atgaaaccaa ctctatatta actttatgcc gtatgatttt aactatggac
     3841 acgggtaaaa tcataccaaa agatattgcg ggaaatgcag tggctgaatc ttctccatta
     3901 gaacataggg agagaatttt gttagcagtt cgtagttatc ttggagagaa tattgaatgg
     3961 actaatgaaa atgtaaattt aactataaac tatttaaata acagattaaa aaaattataa
     4021 aaaaattgaa aaaatggtgg aaacactttt gttattatat tttactggat gaattgtttt
     4081 agtacctaga tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga cttcttccgc
     4141 atcaagtgtt ttggctctca ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg
     4201 gtattcgtgc agggcaagat tcggaatacc aagtacgaga aggacggcca gacggtctac
     4261 gggaccgact tcattgccga taaggtggat tatctggaca ccaaggcacc aggcgggtca
     4321 aatcaggaat aagggcacat tgccccggcg tgagtcgggg caatcccgca aggagggtga
     4381 atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc ggggttttcc
     4441 gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc
     4501 cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa
     4561 ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg tcgtctcgaa
     4621 caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac tatgacgacc
     4681 aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc
     4741 gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt gttcgatatt
     4801 gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc
     4861 accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc
     4921 aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga tgacgaactg
     4981 gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc
     5041 acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta ttacacgaag
     5101 gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt
     5161 gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa
     5221 acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac
     5281 tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg acggatgttc
     5341 gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg
     5401 tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa
     5461 gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct ggtgcattgc
     5521 aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagccagcgc tttac
//
