LOCUS       40924_46903        5818 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pWY107, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Rozwadowski K, Yang W, Kagale S.
  TITLE     Homologous recombination-mediated cloning and manipulation of 
            genomic DNA regions using Gateway and recombineering systems
  JOURNAL   BMC Biotechnol. 8, 88 (2008)
  PUBMED    19014699
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Rozwadowski KL, Yang W, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2008) Molecular Genetics, Agriculture and 
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 
            0X2, Canada
REFERENCE   3  (bases 1 to 5818)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5818)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 8, 88 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2008) Molecular Genetics, Agriculture and Agri-Food Canada, 
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(106..130)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     regulatory      231..733
                     /label=tCUP2 promoter
                     /note="tCUP2 promoter"
                     /regulatory_class="promoter"
     CDS             779..1570
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      1647..1899
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1916..1932)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2013..2037)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      2115..2746
                     /label=oriV
                     /note="incP origin of replication"
     CDS             3589..4263
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /protein_id="ACL35312.1"
                     /translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI
                     GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY
                     QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT
                     MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK
                     KL"
     gene            3589..4263
                     /gene="aad9"
                     /label=aad9
     CDS             4624..5769
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
ORIGIN
        1 tgagatctcc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
       61 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
      121 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gagctccacc
      181 gcggtggcgg ccgctctaga actagccaat tggggcccaa cgttctcgag gggatcttct
      241 gcaagcatct ctatttcctg aaggtctaac ctcgaagatt taagatttaa ttacgtttat
      301 aattacaaaa ttgattctag tatctttaat ttaatgctta tacattatta attaatttag
      361 tactttcaat ttgttttcag aaattatttt actatttttt ataaaataaa agggagaaaa
      421 tggctattta aatactagcc tattttattt caattttagc ttaaaatcag ccccaattag
      481 ccccaatttc aaattcaaat ggtccagccc aattcctaaa taacccaccc ctaacccgcc
      541 cggtttcccc ttttgatcca tgcagtcaac gcccagaatt tccctatata attttttaat
      601 tcccaaacac ccctaactct atcccatttc tcaccaaccg ccacatagat ctatcctctt
      661 atctctcaaa ctctctcgaa ccttccccta accctagcag cctctcatca tcctcacctc
      721 aaaacccacc ggatctagag catcgaattc ctgcagccca gcttgataaa ggccggccat
      781 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      841 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      901 gcaggggagg ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaacttca
      961 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1021 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1081 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     1141 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     1201 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     1261 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     1321 cgaggatctc gtcgtgactc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1381 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1441 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1501 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1561 cgagttcttc tgaggcgcgc cgaattcctg cagcccgggg gatccactag tgatatccct
     1621 gtgtgaaatt gttatccgct acgcgtgatc gttcaaacat ttggcaataa agtttcttaa
     1681 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     1741 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     1801 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     1861 ataaattatc gcgcgcggtg tcatctatgt tactagatcc catgatcaag cttatcgata
     1921 ccgtcgacct cgaggggggg cccggtacca aaaccacccc agtacattaa aaacgtccgc
     1981 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     2041 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     2101 ccatcagtcc actagacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc
     2161 tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc
     2221 cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt
     2281 gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt
     2341 cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg
     2401 agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg
     2461 aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct
     2521 gacagatgag gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc
     2581 atttgcaagg gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt
     2641 aaaccaatat ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc
     2701 acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctctcg agttggcagc
     2761 atcacccata attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
     2821 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
     2881 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
     2941 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     3001 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     3061 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     3121 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     3181 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagc tagccgttcg
     3241 tgaatacatg ttataataac tataactaat aacgtaacgt gactggcaag agatattttt
     3301 aaaacaatga ataggtttac acttacttta gttttatgga aatgaaagat catatcatat
     3361 ataatctaga ataaaattaa ctaaaataat tattatctag ataaaaaatt tagaagccaa
     3421 tgaaatctat aaataaacta aattaagttt atttaattaa caactatgga tataaaatag
     3481 gtactaatca aaatagtgag gaggatatat ttgaatacat acgaacaaat taataaagtg
     3541 aaaaaaatac ttcggaaaca tttaaaaaat aaccttattg gtacttacat gtttggatca
     3601 ggagttgaga gtggactaaa accaaatagt gatcttgact ttttagtcgt cgtatctgaa
     3661 ccattgacag atcaaagtaa agaaatactt atacaaaaaa ttagacctat ttcaaaaaaa
     3721 ataggagata aaagcaactt acgatatatt gaattaacaa ttattattca gcaagaaatg
     3781 gtaccgtgga atcatcctcc caaacaagaa tttatttatg gagaatggtt acaagagctt
     3841 tatgaacaag gatacattcc tcagaaggaa ttaaattcag atttaaccat aatgctttac
     3901 caagcaaaac gaaaaaataa aagaatatac ggaaattatg acttagagga attactacct
     3961 gatattccat tttctgatgt gagaagagcc attatggatt cgtcagagga attaatagat
     4021 aattatcagg atgatgaaac caactctata ttaactttat gccgtatgat tttaactatg
     4081 gacacgggta aaatcatacc aaaagatatt gcgggaaatg cagtggctga atcttctcca
     4141 ttagaacata gggagagaat tttgttagca gttcgtagtt atcttggaga gaatattgaa
     4201 tggactaatg aaaatgtaaa tttaactata aactatttaa ataacagatt aaaaaaatta
     4261 taaaaaaatt gaaaaaatgg tggaaacact tttgttatta tattttactg gatgaattgt
     4321 tttagtacct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc
     4381 cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg
     4441 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
     4501 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
     4561 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
     4621 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
     4681 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
     4741 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
     4801 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc
     4861 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
     4921 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
     4981 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
     5041 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
     5101 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
     5161 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
     5221 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
     5281 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
     5341 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
     5401 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
     5461 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
     5521 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
     5581 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
     5641 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
     5701 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
     5761 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagccag cgctttac
//
