LOCUS       40924_46898        3030 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway Entry vector pWY102, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Rozwadowski K, Yang W, Kagale S.
  TITLE     Homologous recombination-mediated cloning and manipulation of 
            genomic DNA regions using Gateway and recombineering systems
  JOURNAL   BMC Biotechnol. 8, 88 (2008)
  PUBMED    19014699
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Rozwadowski KL, Yang W, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2008) Molecular Genetics, Agriculture and 
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 
            0X2, Canada
REFERENCE   3  (bases 1 to 3030)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3030)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 8, 88 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2008) Molecular Genetics, Agriculture and Agri-Food Canada, 
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    67..166
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             209..919
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     protein_bind    complement(968..1067)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1190..1996
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2089..2677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2842..2928
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3020..3030
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc
       61 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctttaa aggaaccaat
      181 tcagtcgact ggatccaaaa caatggctag taaaggagaa gaacttttca ctggagttgt
      241 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      301 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      361 actacctgtt ccctggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc
      421 aagataccca gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata
      481 cgtgcaggag aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt
      541 caagtttgag ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga
      601 ggacggaaac atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat
      661 catggccgac aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga
      721 agacggcggc gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatggccc
      781 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      841 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
      901 catggatgaa ctatacaaag gtggaggtgg aagatctacc tagggcggcc gcactcgaga
      961 tatctagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1021 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
     1081 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1141 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     1201 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     1261 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     1321 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     1381 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     1441 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     1501 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     1561 gtccctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     1621 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     1681 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     1741 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     1801 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     1861 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     1921 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     1981 gctcgatgag tttttctaat cagaattggt taattggttg taacattatt cagattgggc
     2041 cccgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2341 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2641 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2701 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2761 ttctgtggat aaccgtatta ccgctagcat ggatctcggg gacgtctaac tactaagcga
     2821 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt
     2881 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     2941 gatttgaacg ttgtgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     3001 gccaggcatc aaactaagca gaaggccatc
//
