LOCUS       40924_46798        4819 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWW3840, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in 
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2017)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAR-2017) Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..530
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     terminator      553..580
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(812..2095)
                     /codon_start=1
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71385.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKSGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTPKYALLSTHAGRRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     misc_feature    2255..2266
                     /label=integration site
                     /note="integration site"
     misc_feature    2534..3131
                     /label=split antibiotic resistance marker
                     /note="split antibiotic resistance marker"
     terminator      3147..3173
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      3148..3177
                     /regulatory_class="terminator"
     rep_origin      complement(3279..3867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3945..4048
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4049..4705
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      4779..4813
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gcggccgctg gtctccaatg gtttttactc tggaagattt tgttggcgat tggcgtcaga
       61 ccgcgggtta taatttggat caagtcctgg aacagggtgg cgtaagctct ctgttccaga
      121 acctgggtgt gagcgtgacg ccgattcagc gcatcgttct gtccggcgag aacggtctga
      181 aaattgatat tcatgtgatc atcccgtacg aaggcctgag cggtgaccaa atgggtcaaa
      241 tcgagaaaat ctttaaagtc gtctacccag ttgacgatca ccacttcaag gttatcttgc
      301 attacggtac gctggtgatt gatggtgtga ccccgaatat gattgactat ttcggccgtc
      361 cgtatgaagg cattgccgtt tttgacggta aaaagatcac cgtcaccggt accctgtgga
      421 atggcaataa gattattgac gagcgtctga ttaacccgga cggcagcctg ctgttccgcg
      481 tgaccatcaa cggtgtcacg ggttggcgtc tgtgcgagcg catcctggca taaatcctag
      541 ctgattagct cgagaaggcc atcctgacgg atggcctttt ttttgtatcg agacgactag
      601 tgtaattgcc tatcttccag tgatggaaca gcatttgtgc attggctgca acaatcagcc
      661 ttacttgtgc ctgttctatt tccgaaccga ccgcttgtat gaatccatca aaattcgttt
      721 tctctatgtt ggattccttg ttgctcatat tgtgatgata atttctacaa atatagtcat
      781 tggtaactat ctatgaaact gtttgatact tttatagttg attaaacttg ttcatggcat
      841 ttgccttaat atcatccgct atgtcaatgt agggtttcat agctttgtag tcgctgtgtc
      901 ccgtccattt catgaccacc tgtgccggga ttccgagagc cagcgcattg cagatgaatg
      961 tccttcttcc tgcatgggta ctgagcaaag cgtatttggg tgtgacttca tcaatacgtt
     1021 catttccctt gtagtaggtt tcccgtacag gctcgttgat ttctgccagt tcgcccagct
     1081 ctttcaggta atcgttcatc ttctggttgc tgatgacggg cagagccatg taattctcga
     1141 aatggatgtc cttgtatttg tccagtatgg ctttgctgta tttgttcagt tcaatcgtca
     1201 ggctgtcggc agtcttgact gtggttattt cgatgtggtc ggacttcaca tcgcttcttt
     1261 tcagattgcg aacatccgaa taccgcaaac tcgtaaagca gcagaacagg aaaacatcac
     1321 gcacacgttc caggtattgc ttatccttgg gtatctggta gtctttcagc ttgttcagtt
     1381 catcccaagt caggaagatt acttttttcg aggtggtttt cagtttcggt ttgaacgtat
     1441 cgtatgcaat gttctgatga tgtcctttct tgaagctcca gcgcaggaac catttgagga
     1501 atcccatttg cttgccgatg gtgctgtttc tcatatcctt ggtgtcacgc aggaagttga
     1561 cgtattcgtt caatccaaac tcgttgaaat agttgaacgt tgcatcctcc ttgaactctt
     1621 tgaggtggtt cctcactgct gcaaattttt cataggtgga tgccgtccag ttattctggt
     1681 taccgcactc ttttacaaac tcatcgaaca cctcccaaaa gctgacaggg gcttcttccg
     1741 gctgttcttc gctggtgtct ttcattctca tgttgaaagc ttccttcaac tgttgggtcg
     1801 ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt ctggatttcg gcatagtatt
     1861 tcagcaagtc cgtattgatt tcggctgcac tttgctttag cttgttggta catccgctct
     1921 ttacccgctg cttatctgca tcccatttgg ctacgtcaat ccggtagccc gttgtaaact
     1981 cgatgcgttg gctggcaaag atgacacgca tacggatggg tacgttctct acgattggca
     2041 caccgttctt tttccggctc tccaatgcaa aaatgatgtt gcgcttgata ttcataattg
     2101 ggtgcgtttg aaattctaca cccaaatata cacccaatta ttgagatagc aaaagacatt
     2161 tagaaacatt tacttttact ctatattgta atttacactt gattatcagt cgtttgcagt
     2221 cttatgatat tctgtgaaag tataagttcg agagcctgtc tctccgcaaa aaacgctgaa
     2281 aatcagcaga ttgcaaaaca aacaccctgt tttacaccca agaatgtaaa gtcggctgtt
     2341 tttgttttat ttaagataat acaaccacta cataataaaa gagtagcgat attaaaagaa
     2401 tccgatgaga aaagactaat atttatctat ccattcagtt tgatttttca ggactttaca
     2461 tcgtcctgaa agtatttgtt ggtaccgagg acgcgacagc tgcagacgcg ttatgagtaa
     2521 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2581 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2641 ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat
     2701 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2761 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2821 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2881 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2941 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3001 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3061 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3121 cggcgaccga gtgagaccac ctgcaggaag gccatcctga cggatggcct tttttttaga
     3181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgattgatcg ggcacgtaag
     3961 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
     4021 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     4081 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     4141 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     4201 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     4261 ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     4321 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     4381 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     4441 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtttcagcca atccctgggt
     4501 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     4561 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     4621 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     4681 ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga
     4741 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
     4801 gggcgttttt tattggtag
//
