LOCUS       40924_46788        5014 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWW3828, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in 
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5014)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2017)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAR-2017) Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..725
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      748..775
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(1007..2290)
                     /codon_start=1
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71379.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKSGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTPKYALLSTHAGRRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     misc_feature    2450..2461
                     /label=integration site
                     /note="integration site"
     misc_feature    2729..3326
                     /label=split antibiotic resistance marker
                     /note="split antibiotic resistance marker"
     terminator      3342..3368
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      3343..3372
                     /regulatory_class="terminator"
     rep_origin      complement(3474..4062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4140..4243
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4244..4900
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      4974..5008
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gcggccgctg gtctccaatg gtttcgaaag gggaagaaga taacatggct atcattaagg
       61 agtttatgcg cttcaaggtg cacatggagg gctctgtcaa cggccacgag ttcgaaatcg
      121 aaggtgaagg tgaaggtcgt ccgtacgaag gtactcagac cgcaaagttg aaagttacaa
      181 aaggaggtcc gttgcctttc gcttgggaca ttctgagccc gcaatttatg tacgggagca
      241 aagcctatgt taaacatccg gcggatattc ccgattacct gaaactgtca ttcccggagg
      301 ggttcaagtg ggaacgcgtc atgaactttg aagacggtgg cgtggttaca gtgactcagg
      361 attcgtccct tcaagatggc gaatttatct acaaagtcaa actgcgcggg acaaattttc
      421 cgagtgacgg tcctgttatg cagaagaaga ccatggggtg ggaagcaagc tccgaacgca
      481 tgtaccccga ggacggtgca ctcaaaggag aaatcaagca gcgtctgaaa ctgaaagatg
      541 gcgggcacta cgatgcagaa gtgaaaacca cctataaggc gaaaaagcct gtacaactgc
      601 cgggtgccta taatgtgaac attaaactgg atataacgag tcacaacgaa gactatacaa
      661 tcgtcgagca gtatgagcgt gcggaaggac gtcattccac tggtggaatg gatgaactgt
      721 ataagtaaat cctagctgat tagctcgaga aggccatcct gacggatggc cttttttttg
      781 tatcgagacg actagtgtaa ttgcctatct tccagtgatg gaacagcatt tgtgcattgg
      841 ctgcaacaat cagccttact tgtgcctgtt ctatttccga accgaccgct tgtatgaatc
      901 catcaaaatt cgttttctct atgttggatt ccttgttgct catattgtga tgataatttc
      961 tacaaatata gtcattggta actatctatg aaactgtttg atacttttat agttgattaa
     1021 acttgttcat ggcatttgcc ttaatatcat ccgctatgtc aatgtagggt ttcatagctt
     1081 tgtagtcgct gtgtcccgtc catttcatga ccacctgtgc cgggattccg agagccagcg
     1141 cattgcagat gaatgtcctt cttcctgcat gggtactgag caaagcgtat ttgggtgtga
     1201 cttcatcaat acgttcattt cccttgtagt aggtttcccg tacaggctcg ttgatttctg
     1261 ccagttcgcc cagctctttc aggtaatcgt tcatcttctg gttgctgatg acgggcagag
     1321 ccatgtaatt ctcgaaatgg atgtccttgt atttgtccag tatggctttg ctgtatttgt
     1381 tcagttcaat cgtcaggctg tcggcagtct tgactgtggt tatttcgatg tggtcggact
     1441 tcacatcgct tcttttcaga ttgcgaacat ccgaataccg caaactcgta aagcagcaga
     1501 acaggaaaac atcacgcaca cgttccaggt attgcttatc cttgggtatc tggtagtctt
     1561 tcagcttgtt cagttcatcc caagtcagga agattacttt tttcgaggtg gttttcagtt
     1621 tcggtttgaa cgtatcgtat gcaatgttct gatgatgtcc tttcttgaag ctccagcgca
     1681 ggaaccattt gaggaatccc atttgcttgc cgatggtgct gtttctcata tccttggtgt
     1741 cacgcaggaa gttgacgtat tcgttcaatc caaactcgtt gaaatagttg aacgttgcat
     1801 cctccttgaa ctctttgagg tggttcctca ctgctgcaaa tttttcatag gtggatgccg
     1861 tccagttatt ctggttaccg cactctttta caaactcatc gaacacctcc caaaagctga
     1921 caggggcttc ttccggctgt tcttcgctgg tgtctttcat tctcatgttg aaagcttcct
     1981 tcaactgttg ggtcgttggc atgacctcct gcacctcaaa ttccttgaaa atattctgga
     2041 tttcggcata gtatttcagc aagtccgtat tgatttcggc tgcactttgc tttagcttgt
     2101 tggtacatcc gctctttacc cgctgcttat ctgcatccca tttggctacg tcaatccggt
     2161 agcccgttgt aaactcgatg cgttggctgg caaagatgac acgcatacgg atgggtacgt
     2221 tctctacgat tggcacaccg ttctttttcc ggctctccaa tgcaaaaatg atgttgcgct
     2281 tgatattcat aattgggtgc gtttgaaatt ctacacccaa atatacaccc aattattgag
     2341 atagcaaaag acatttagaa acatttactt ttactctata ttgtaattta cacttgatta
     2401 tcagtcgttt gcagtcttat gatattctgt gaaagtataa gttcgagagc ctgtctctcc
     2461 gcaaaaaacg ctgaaaatca gcagattgca aaacaaacac cctgttttac acccaagaat
     2521 gtaaagtcgg ctgtttttgt tttatttaag ataatacaac cactacataa taaaagagta
     2581 gcgatattaa aagaatccga tgagaaaaga ctaatattta tctatccatt cagtttgatt
     2641 tttcaggact ttacatcgtc ctgaaagtat ttgttggtac cgaggacgcg acagctgcag
     2701 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc
     2881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3301 tgagaatagt gtatgcggcg accgagtgag accacctgca ggaaggccat cctgacggat
     3361 ggcctttttt ttagacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgatt
     4141 gatcgggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
     4201 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     4261 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     4321 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     4381 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     4441 gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga
     4501 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     4561 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     4621 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtttc
     4681 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     4741 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     4801 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     4861 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc
     4921 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     4981 cgctcggttg ccgccgggcg ttttttattg gtag
//
