LOCUS       40924_46778        4307 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWW3810, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in 
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4307)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4307)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2017)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAR-2017) Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      35..62
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(294..1577)
                     /codon_start=1
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71375.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKSGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTPKYALLSTHAGRRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     misc_feature    1737..1748
                     /label=integration site
                     /note="integration site"
     misc_feature    2022..2619
                     /label=split antibiotic resistance marker
                     /note="split antibiotic resistance marker"
     terminator      2635..2661
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      2636..2665
                     /regulatory_class="terminator"
     rep_origin      complement(2767..3355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3433..3536
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3537..4193
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      4267..4301
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gcggccgcgg tctccatcct agctgattag ctcgagaagg ccatcctgac ggatggcctt
       61 ttttttgtat cgagacgact agtgtaattg cctatcttcc agtgatggaa cagcatttgt
      121 gcattggctg caacaatcag ccttacttgt gcctgttcta tttccgaacc gaccgcttgt
      181 atgaatgcat caaaattcgt tttctctacg ttggattcct tgttgctcat attgtgatga
      241 taatttctac aaatatagtc attggtaact atctatgaaa ctgtttgata cttttatagt
      301 tgattaaact tgttcatggc atttgcctta atatcatccg ctatgtcaat gtagggtttc
      361 atagctttgt agtcgctgtg tcccgtccat ttcatgacca cctgtgccgg gattccgaga
      421 gccagcgcat tgcagatgaa tgtccttctt cctgcatggg tactgagcaa agcgtatttg
      481 ggtgtgactt catcaatacg ttcatttccc ttgtagtagg tttcccgtac aggctcgttg
      541 atttctgcca gttcgcccag ctctttcagg taatcgttca tcttctggtt gctgatgacg
      601 ggcagagcca tgtaattctc gaaatggatg tccttgtatt tgtccagtat ggctttgctg
      661 tatttgttca gttcaatcgt caggctgtcg gcagtcttga ctgtggttat ttcgatgtgg
      721 tcggacttca catcgcttct tttcagattg cgaacatccg aataccgcaa actcgtaaag
      781 cagcagaaca ggaaaacatc acgcacacgt tccaggtatt gcttatcctt gggtatctgg
      841 tagtctttca gcttgttcag ttcatcccaa gtcaggaaga ttactttttt cgaggtggtt
      901 ttcagtttcg gtttgaacgt atcgtatgca atgttctgat gatgtccttt cttgaagctc
      961 cagcgcagga accatttgag gaatcccatt tgcttgccga tggtgctgtt tctcatatcc
     1021 ttggtgtcac gcaggaagtt gacgtattcg ttcaatccaa actcgttgaa atagttgaac
     1081 gttgcatcct ccttgaactc tttgaggtgg ttcctcactg ctgcaaattt ttcataggtg
     1141 gatgccgtcc agttattctg gttaccgcac tcttttacaa actcatcgaa cacctcccaa
     1201 aagctgacag gggcttcttc cggctgttct tcgctggtgt ctttcattct catgttgaaa
     1261 gcttccttca actgttgggt cgttggcatg acctcctgca cctcaaattc cttgaaaata
     1321 ttctggattt cggcatagta tttcagcaag tccgtattga tttcggctgc actttgcttt
     1381 agcttgttgg tacatccgct ctttacccgc tgcttatctg catcccattt ggctacgtca
     1441 atccggtagc ccgttgtaaa ctcgatgcgt tggctggcaa agatgacacg catacggatg
     1501 ggtacgttct ctacgattgg cacaccgttc tttttccggc tctccaatgc aaaaatgatg
     1561 ttgcgcttga tattcataat tgggtgcgtt tgaaattcta cacccaaata tacacccaat
     1621 tattgagata gcaaaagaca tttagaaaca tttactttta ctctatattg taatttacac
     1681 ttgattatca gtcgtttgca gtcttatgat attctgtgaa agtataagtt cgagagcctg
     1741 tctctccgca aaaaacgctg aaaatcagca gattgcaaaa caaacaccct gttttacacc
     1801 caagaatgta aagtcggctg tttttgtttt atttaagata atacaaccac tacataataa
     1861 aagagtagcg atattaaaag aatccgatga gaaaagacta atatttatct atccattcag
     1921 tttgattttt caggacttta catcgtcctg aaagtatttg ttggtaccgg taccgaggac
     1981 gcgacagctg cagacgcgtt atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2161 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt gagaccacct gcaggaaggc
     2641 catcctgacg gatggccttt tttttagacg gttatccaca gaatcagggg ataacgcagg
     2701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3421 tttggtcatg attgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat
     3481 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
     3541 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     3601 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     3661 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     3721 ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc
     3781 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     3841 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     3901 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     3961 tgtttttcgt ttcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     4021 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     4081 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     4141 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg
     4201 atatcgagct cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
     4261 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtag
//
