LOCUS       40924_46773        4072 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWW3807, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in 
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4072)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4072)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2017)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAR-2017) Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..284
                     /label=split antibiotic resistance marker
                     /note="split antibiotic resistance marker"
     CDS             772..1506
                     /codon_start=1
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71374.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGSIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      complement(1576..1964)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            2135..2244
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      2399..2426
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(2532..3120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3198..3301
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3302..3958
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      4032..4066
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gcggccgctg gtctcccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
       61 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      301 tcatggccgc gggattaaaa gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag
      361 agaaatatcg ttttgctaaa cagttgatat tgaggtatca ttttatcgta aaagacattt
      421 ttgctcaaca attgcttgac ggaaatcaac aaattttagc attttgtaaa aaagtcgcta
      481 tataatttgg tgaattggag ttattttcat atttttgcat cccgaagagt ttctcttaaa
      541 gagagaaaca tcttttgcat accttttccg accgaatttt tatgtcgtaa agaggggctt
      601 tgcagggggt ggactcagaa agatgagaat agatgactat tgtagttgaa acacatagaa
      661 agttgctgat atacagaccg atacgcatat cgggatgaac catgagtacg ttcttttctc
      721 aaaaaacata aatattcgaa aagagatgca ataaattaag gagaggttat aatgaacaaa
      781 gtaaatataa aagatagtca aaattttatt acttcaaaat atcacataga aaaaataatg
      841 aattgcataa gtttagatga aaaagataac atctttgaaa taggtgcagg gaaaggtcat
      901 tttactgctg gattggtaaa gagatgtaat tttgtaacgg cgatagaaat tgattctaaa
      961 ttatgtgagg taactcgtaa taagctctta aattatccta actatcaaat agtaaatgat
     1021 gatatactga aatttacatt tcctagccac aatccatata aaatatttgg cagcatacct
     1081 tacaacataa gcacaaatat aattcgaaaa attgtttttg aaagttcagc cacaataagt
     1141 tatttaatag tggaatatgg ttttgctaaa atgttattag atacaaacag atcactagca
     1201 ttgctgttaa tggcagaggt agatatttct atattagcaa aaattcctag gtattatttc
     1261 catccaaaac ctaaagtgga tagcacatta attgtattaa aaagaaagcc agcaaaaatg
     1321 gcatttaaag agagaaaaaa atatgaaact tttgtaatga aatgggttaa caaagagtac
     1381 gaaaaactgt ttacaaaaaa tcaatttaat aaagctttaa aacatgcgag aatatatgat
     1441 ataaacaata ttagtttcga acaatttgta tcgctattta atagttataa aatatttaac
     1501 ggctaaaaac aataggccac atgcaactgt aaatgtttac gcgggtaccg acaccgcggt
     1561 ggaggggaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga
     1621 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
     1681 taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg
     1741 ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga
     1801 aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt
     1861 tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag
     1921 cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca
     1981 tcgtggccgg atcaattccg ttttccgctg cataaccctg cttcggggtc attatagcga
     2041 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     2101 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     2161 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     2221 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
     2281 ctgatgaaac caagccaacc aggaagggca gcccacctat cacggaattg atccccctcg
     2341 aattggaatt cgttccgtct cgattcagat ctaaaggatc caatctatga gacctgcagg
     2401 aaggccatcc tgacggatgg cctttttttt agacggttat ccacagaatc aggggataac
     2461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2941 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3181 gggattttgg tcatgattga tcgggcacgt aagaggttcc aactttcacc ataatgaaat
     3241 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     3301 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     3361 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     3421 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     3481 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     3541 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     3601 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     3661 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     3721 gaatatgttt ttcgtttcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     3781 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     3841 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     3901 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     3961 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     4021 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt ag
//
