LOCUS       40924_46768        4243 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWW3806, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in 
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4243)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4243)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2017)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAR-2017) Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..284
                     /label=split antibiotic resistance marker
                     /note="split antibiotic resistance marker"
     CDS             772..1506
                     /codon_start=1
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71373.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGSIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      complement(1576..1964)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            2135..2244
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(2389..2432)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2570..2597
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(2703..3291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3369..3472
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3473..4129
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      4203..4237
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gcggccgctg gtctcccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
       61 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      301 tcatggccgc gggattaaaa gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag
      361 agaaatatcg ttttgctaaa cagttgatat tgaggtatca ttttatcgta aaagacattt
      421 ttgctcaaca attgcttgac ggaaatcaac aaattttagc attttgtaaa aaagtcgcta
      481 tataatttgg tgaattggag ttattttcat atttttgcat cccgaagagt ttctcttaaa
      541 gagagaaaca tcttttgcat accttttccg accgaatttt tatgtcgtaa agaggggctt
      601 tgcagggggt ggactcagaa agatgagaat agatgactat tgtagttgaa acacatagaa
      661 agttgctgat atacagaccg atacgcatat cgggatgaac catgagtacg ttcttttctc
      721 aaaaaacata aatattcgaa aagagatgca ataaattaag gagaggttat aatgaacaaa
      781 gtaaatataa aagatagtca aaattttatt acttcaaaat atcacataga aaaaataatg
      841 aattgcataa gtttagatga aaaagataac atctttgaaa taggtgcagg gaaaggtcat
      901 tttactgctg gattggtaaa gagatgtaat tttgtaacgg cgatagaaat tgattctaaa
      961 ttatgtgagg taactcgtaa taagctctta aattatccta actatcaaat agtaaatgat
     1021 gatatactga aatttacatt tcctagccac aatccatata aaatatttgg cagcatacct
     1081 tacaacataa gcacaaatat aattcgaaaa attgtttttg aaagttcagc cacaataagt
     1141 tatttaatag tggaatatgg ttttgctaaa atgttattag atacaaacag atcactagca
     1201 ttgctgttaa tggcagaggt agatatttct atattagcaa aaattcctag gtattatttc
     1261 catccaaaac ctaaagtgga tagcacatta attgtattaa aaagaaagcc agcaaaaatg
     1321 gcatttaaag agagaaaaaa atatgaaact tttgtaatga aatgggttaa caaagagtac
     1381 gaaaaactgt ttacaaaaaa tcaatttaat aaagctttaa aacatgcgag aatatatgat
     1441 ataaacaata ttagtttcga acaatttgta tcgctattta atagttataa aatatttaac
     1501 ggctaaaaac aataggccac atgcaactgt aaatgtttac gcgggtaccg acaccgcggt
     1561 ggaggggaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga
     1621 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
     1681 taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg
     1741 ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga
     1801 aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt
     1861 tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag
     1921 cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca
     1981 tcgtggccgg atcaattccg ttttccgctg cataaccctg cttcggggtc attatagcga
     2041 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     2101 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     2161 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     2221 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
     2281 ctgatgaaac caagccaacc aggaagggca gcccacctat cacggaattg atccccctcg
     2341 aattggaatt cgttccgtct cgattcagat ctaaaggatc caatctagat aaaacgaaag
     2401 gctcagtcga aagactgggc ctttcgtttt acaattgggc tacctttttt ttgttttgtt
     2461 tgcaatggtt aatctattgt taaaatttaa agtttcactt gaactttcaa ataatgttct
     2521 tatatttgca gtgtcgaaag aaacaaagta ggacttgaga ccacctgcag gaaggccatc
     2581 ctgacggatg gccttttttt tagacggtta tccacagaat caggggataa cgcaggaaag
     2641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3301 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3361 gtcatgattg atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact
     3421 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     3481 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     3541 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     3601 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     3661 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     3721 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     3781 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     3841 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     3901 tttcgtttca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     3961 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     4021 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     4081 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatttgatat
     4141 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     4201 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tag
//
