LOCUS       V002097                 5823 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002097
VERSION     V002097
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Tunable expression tools enable single-cell strain distinction in
            the gut microbiome
  JOURNAL   Cell (2017) In press
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Whitaker WR, Shepherd ES, Sonnenburg JL.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2017)
            In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-MAR-2017) Microbiology "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..284
                     /label="split antibiotic resistance marker"
                     /note="split antibiotic resistance marker"
     promoter        complement(285..389)
                     /label="AmpR promoter"
     CDS             complement(811..2733)
                     /gene="tetQ"
                     /label="Tetracycline resistance protein TetQ"
                     /note="Tetracycline resistance protein TetQ from
                     Bacteroides fragilis. Accession#: Q08425"
     rep_origin      complement(3327..3715)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            3886..3995
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      4150..4177
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4949..5052
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5053..5709
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      5783..5817
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gcggccgctg gtctcccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
       61 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgctaag
      421 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
      481 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
      541 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      601 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      661 accatacgtc tattttttta ttgccaagtt ctaatgcttc tatctcctgc cattcataga
      721 ggctattata tgccatacat ccgataagaa tggagataag tatatatccc aactgtattg
      781 ccttatagaa atttcctgac cgctccatta ttttgatgac attgattttt ggaacatgaa
      841 taaaagttta tctttttcgt tcatgcggat attatcagaa taaccgcctt ttgttatttg
      901 atacccgcat ggcttaacca taaaaatgcc taagccctta gtgtatgaac ttacttctga
      961 tgcatagtct ttacttgtat ttaatggaac tttcccttta atatgacacc actcattatt
     1021 gcaactgatg tcttcaatct cagacatcat tttttgcaaa tctgtaatag ctttggaact
     1081 tgccgcttgg ggtatctgca actcaaaata gagcatcggt tcgagaatgt ccacacctga
     1141 ctgttgcaag gccagcctga agacataagg ggtcagctgt ctgaaatcag caggtgtact
     1201 taccgggcta taatactcgg cttgagtaaa agttactttc agatcagtca cttcccatcc
     1261 atgtaacccg gattggcaag acatacgaat cccttcaaaa acggcatttt gaaaagaatg
     1321 gttcagataa ccataggaga tgtcactttc gatttgcaac cctgtcccta acggtaaggg
     1381 ttcaagagtc agccctattg tggcccaata agggttgggc ggcacttcga tctgaataat
     1441 cttattgacc ttttttacag gtcgttcttt gtatatagtc ttgatctcat caaaatggac
     1501 ctttacggaa aatcgttctt ccagcaatgt ctgtatgatt tccttttggg ttaaaccata
     1561 taacgagatt tccaattcat cactatatga gtttatggaa aaggacaaag acgggtcttc
     1621 aatccacaat gtattcagag cggatatcac cttgcttctc tcttcgggcc tgtctggccg
     1681 gacggaggat ttgagagcgg gatgctgatg cgataatcct tgaatcaaac aaggttcagc
     1741 acctaaataa tttccgattc gaaaatcatc catatcctct acaatcgcga tatcattggc
     1801 gcccacttca tcaacattta tctctctgcc ctgattgata gtttttagat ttttaatctt
     1861 gatgaatttt tccgaatcgt tgattcttac aacgtctcga agtctcagac ttccgtcaat
     1921 tatttttaga aaacttcttt tatgtccttt ggggtcatgc tctatcttat aaagataaga
     1981 tgaaagtctg tttgaaaccg atgccggagg aagtataaaa gaagtgatgg cgtccaacaa
     2041 ctcattgata ccgatattga acattgctga tccatgtagc accggataga ctttggcttt
     2101 tgccacaaga gcgattatcg tattccaata atcagccggt gaaatttcgc tatccgccaa
     2161 atatcgttct aatatattgt cgtcatggtt gcatacaaat tctttgtatt cttcctttat
     2221 atatgtttgg gagcaaaccg gataaaccga tccatcgaca acattttgca taaacaggac
     2281 atcttgagac agatttgctt ttatatccag atacaaacgc tccaaattca caccggctcg
     2341 gtcaatctta ttgataaata taattgtcgg gatttgcagc ttctgtaaag tattgaacag
     2401 caactttgtc tgcgcttgta tgccttcctt tgcggataag atgaggactg ctccatcaag
     2461 cattttgaat gtccgctcca cttccgcaat aaaatccatg tgtcccggag tgtcaatgat
     2521 attgcatttc acaccattcc agataataga tgtcgtagaa gcccgaacag taattcctct
     2581 acgtttctct atatccatag agtccgttat ggtgtcacca ttatccacac agccgcactt
     2641 ttccgttgct ccactggcaa acagcagatt ctcggttacg gaagtttttc ctgcatcaat
     2701 gtgagcaaga attcctaaat ttataatatt catttggatt aagcaataat atactacaat
     2761 agatgcattg tcgaaacgca ccttttaata cctcctcgta gcatatgaga actacaggat
     2821 tcctaactcg tattaatatg tatattatta ctgccgcata acgattacaa aattacacaa
     2881 aaaaatatat ctaacaaaag taggaggatt ttttaacttt ttaccataga cattttatta
     2941 gggaaacgta ggttcaactg gtaagtacaa ataagtagaa atgtttgaac aacactgttt
     3001 taggaagttg aaaaatcaag tttctctctc ggtatagcgt ttattttggc caatatcttc
     3061 tttagtttgg catttgagta tttcccattc gtgttctatt tagctcctta ttatgagtat
     3121 gtagcaagtt gcttatcaaa ttcagcctct ttgagggtca aatatggttt tgcaaatggt
     3181 gactgacaag acataaacaa ggtcagcagg attttttaca taaatggaca tgaatgtata
     3241 taacggggtt tgagggccaa tggaacgaaa acgtacgtta aggagataac gcgtggtacc
     3301 gacaccgcgg tggaggggaa ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa
     3361 ttgctttgag aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa
     3421 ccgttaaacc ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa
     3481 aaccttagag gctatttaag ttgctgattt atattaattt tattgttcaa acatgagagc
     3541 ttagtacgtg aaacatgaga gcttagtacg ttagccatga gagcttagta cgttagccat
     3601 gagggtttag ttcgttaaac atgagagctt agtacgttaa acatgagagc ttagtacgtg
     3661 aaacatgaga gcttagtacg tactatcaac aggttgaact gctgatcttc agatcctcta
     3721 cgccggacgc atcgtggccg gatcaattcc gttttccgct gcataaccct gcttcggggt
     3781 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca
     3841 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga
     3901 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt
     3961 gctcaacggg aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg
     4021 caagcggatg gctgatgaaa ccaagccaac caggaagggc agcccaccta tcacggaatt
     4081 gatccccctc gaattggaat tcgttccgtc tcgattcaga tctaaaggat ccaatctatg
     4141 agacctgcag gaaggccatc ctgacggatg gccttttttt tagacggtta tccacagaat
     4201 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4261 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4321 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4381 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4441 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4501 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4561 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4621 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4681 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4741 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4801 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4861 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4921 actcacgtta agggattttg gtcatgattg atcgggcacg taagaggttc caactttcac
     4981 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     5041 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     5101 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     5161 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     5221 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
     5281 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     5341 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     5401 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     5461 gggtttattg agaatatgtt tttcgtttca gccaatccct gggtgagttt caccagtttt
     5521 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     5581 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     5641 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     5701 ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc cagtaatgac
     5761 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     5821 tag
//
