LOCUS       40924_46693        7212 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pWW102B, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Wang W, Attia AS, Liu L, Rosche T, Wagner NJ, Hansen EJ.
  TITLE     Development of a shuttle vector for Moraxella catarrhalis
  JOURNAL   Plasmid 55 (1), 50-57 (2006)
  PUBMED    16188314
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Wang W, Attia AS, Liu L, Rosche T, Wagner NJ, Hansen EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2004) Microbiology, University of Texas 
            Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 
            75390, USA
REFERENCE   3  (bases 1 to 7212)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7212)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2006"; volume: "55"; issue: "1"; pages: "50-57"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-SEP-2004) Microbiology, University of Texas Southwestern Medical
            Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(588..1133)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6515..7171)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(7172..7212,1..62))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
       61 caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag
      121 gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt
      181 cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
      241 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
      301 aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg
      361 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca
      421 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt
      481 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
      541 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
      601 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
      661 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
      721 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
      781 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
      841 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
      901 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
      961 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     1021 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     1081 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     1141 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
     1201 cacctgaagt cagccccata cgatataagt tgtaattctc atgtttgaca gcttatcatc
     1261 gataagctag cttgcatgcc tgcaggtcga cggatctatc gattttcgtt cgtgaataca
     1321 tgttataata actataacta ataacgtaac gtgactggca agagatattt ttaaaacaat
     1381 gaataggttt acacttactt tagttttatg gaaatgaaag atcatatcat atataatcta
     1441 gaataaaatt aactaaaata attattatct agataaaaaa tttagaagcc aatgaaatct
     1501 ataaataaac taaattaagt ttatttaatt aacaactatg gatataaaat aggtactaat
     1561 caaaatagtg aggaggatat atttgaatac atacgaacaa attaataaag tgaaaaaaat
     1621 acttcggaaa catttaaaaa ataaccttat tggtacttac atgtttggat caggagttga
     1681 gagtggacta aaaccaaata gtgatcttga ctttttagtc gtcgtatctg aaccattgac
     1741 agatcaaagt aaagaaatac ttatacaaaa aattagacct atttcaaaaa aaataggaga
     1801 taaaagcaac ttacgatata ttgaattaac aattattatt cagcaagaaa tggtaccgtg
     1861 gaatcatcct cccaaacaag aatttattta tggagaatgg ttacaagagc tttatgaaca
     1921 aggatacatt cctcagaagg aattaaattc agatttaacc ataatgcttt accaagcaaa
     1981 acgaaaaaat aaaagaatat acggaaatta tgacttagag gaattactac ctgatattcc
     2041 attttctgat gtgagaagag ccattatgga ttcgtcagag gaattaatag ataattatca
     2101 ggatgatgaa accaactcta tattaacttt atgccgtatg attttaacta tggacacggg
     2161 taaaatcata ccaaaagata ttgcgggaaa tgcagtggct gaatcttctc cattagaaca
     2221 tagggagaga attttgttag cagttcgtag ttatcttgga gagaatattg aatggactaa
     2281 tgaaaatgta aatttaacta taaactattt aaataacaga ttaaaaaaat tataaaaaaa
     2341 ttgaaaaaat ggtggaaaca cttttttcaa tttttttgtt ttattattta atatttggga
     2401 aatattcatt ctaattggta atcagatttt agaaaacaat aaacccttgc atatgattcg
     2461 acggatcttg tacaaaatgg gaaatttgtt ttgccaaacc gtctacaaaa ggttcattgt
     2521 taatgcgatt ataaacaatg gttgcaccat gcttcatatg atgataaatg gcagatttaa
     2581 taaaacgata gcgagtacgc cccatgtcat caaagctttc tacatactca gaaatgggta
     2641 cgatttcacc ctttctaagg cgaaaaagct catctgtggg attggctcgc tgatataatt
     2701 cgttgatttg cttccaagta atattcaaat ctttgactgc gcccttgaat actttgggtg
     2761 cttggtatag cacttgctac ctaaaatctt gagatgacat accaagatct atgtgcatat
     2821 aatacatccc tattaaatta aattaaaaca ttttataaaa atttttatca ttatattcaa
     2881 ataaaggcaa tttgacaaca cagaataaca aaagccctag ataaggggat aaaaattggg
     2941 attttgggcg ttgattttct agttacccct acaagttttg tatttgctaa aatttttgat
     3001 ttatcatatt gcagataaag aaaaacccac cataaggcgg gcttttcgct taatactagg
     3061 gggtaggcgt aagccctcaa tagaccctag caaacagtaa tattttaagg ctttatctct
     3121 atgaaatcaa gtgaaaatta tccaaacatc aaaaacgctt tatcacatcc acgccttgcc
     3181 acctatgaaa aatataccca aaacaccgaa caggcattaa gtttgtatca atggaatttg
     3241 caaatatcgt ccgccttgtt tgagtgtttg gcggtgtgtg aagtggttat tagaaatgcc
     3301 gttgccaatg ccatacaagc tgaacatggt gcagattggg ctttcaatgt gcgatttgtc
     3361 cgaagtatgc aacaaaaccg tcgaaatgaa ctcctaaacg cacgtcaaga aaccacagaa
     3421 cagaccattt gtgaattgcc gtttgtgttt tggcagggat tttttactgt tcgctttgat
     3481 gatgaaattt ggaaaaagca ttgggcggtt gccttgccca atgccaagca cgcagattta
     3541 caagcgatgc gaaccgaagt atttgagaac ctagaaaaaa tccgcaagct aagaaaccgc
     3601 attgcccacc acgaacccat atttaaccgc aatctacaag gcgattacaa gcggattatc
     3661 aaaattatta gctactgctc acaagatacc gcagactggg taaaatcgtg tcaaaggatt
     3721 gatgaattat taaaaatcaa gcctatactc ccataacttg cttaaacgct ccacgataca
     3781 ttaaatcttt gtgggacgag ccatttttga cagatttatc aaaaaattgc tttaaaaatg
     3841 gctcaatagt acgcccaact tcatcaataa tccgagtttg ttcatcttgg ggtaacctct
     3901 caaaataagc gatgaattca gcattttcag cttcttggcg ttgtttttct gcttgctctg
     3961 ctagtttggc ttgtttttca cgctccgctt gctccttgcg tgctaggcgt tcattttcgg
     4021 ctttttgcaa ctgctcataa tgttcatcaa gcccccaacg gtcggcaatg gctttttgct
     4081 caatgttttt tctatgaaaa tcgttcacta attcgccctt actctccaaa tggacgatat
     4141 agtcagcgac tttttggtgg acgatagcaa gttctttgtc ggtcaaacca tgtatgatat
     4201 ccaccgtgtt agggtctcgg ctatcgttaa tcatcttatc aattttggcg gttttggtgc
     4261 gtttatcgtt gataatcagc gtaaaatgcg tgatgatttt gcctattttt tcctgttcaa
     4321 aggttaattc aaattcgcct tgttcgttaa tttcttttat aacaggagtc aaaacatatc
     4381 tcttgaaatc attaactgca ccatacttgt taatacagcc aaaaaaatct ttccattcat
     4441 caaccaccat tgttactttt gggtattttt taccatgtgg cggctttgaa ccagctcgtt
     4501 tacaacacaa ttcataaagg cgttttgtat gaaaacgctt taagtctatg gttttttcgt
     4561 aggggtattt ggtataagct tcccctgttt tttgaattaa atatgccaaa acatctgaaa
     4621 acatcaaata aataaaaccg ctcttttctt ggtaggctac cgcctgcacc caattagcgt
     4681 gcaagtctaa caactcataa tcaccttttg ggcgtgctac actgtacggg tcttcttccc
     4741 ctaccaactt cttcaaacca tctttataaa ttttggcttt aaaagttcgt ttttgtagcc
     4801 ttttcgctgt ctcagccaaa acttcatagg cataattttc attaatttga ttggcaatcg
     4861 caaactcttt ggcagagata aaaacctgct taccaccaac gccttgacta aatagcttgt
     4921 taattgccaa agtcattaaa tcatgttcgg ctggcgacaa gtcaaaagca ccttcaacca
     4981 acgcatttga tttaaaaatt tcaatttctt tgccttcaac ataaaagctg gcattttgga
     5041 caaaatcgga cataaccact aaccatattc aaaaattagg ttcattgtat caaacggaaa
     5101 ttatataatc aattcctttt acggaaaaaa aaatataact ccgtttgatt tcaggggttt
     5161 ctccgtttga tttcaggggt ttctccgttt gatttcaggg gtttctccgt ttgatttcag
     5221 gggtttctcc gtttataatc atgtaaacta ttgattttaa aaagaaaaaa cgcccctata
     5281 atataataat atataataat taaataatac cgtcctcttt ttgccaaaat tttcaatttt
     5341 aaccctaaaa accactttct tttgtctttt caacagatga aaaccatagt aaaattaaca
     5401 aaaaatagta aagggcaagc atacgcagag ttactccatt tcatttcgta acctgcgtgc
     5461 ttggcaggga caagccccta caaccccctc caagtgccag ctgtcttggt ttatttgtag
     5521 ttgtttttgt ctctgtagag tacgcccttt tatactgtag tctataattt attttgttag
     5581 acaaatttct ataaaaatat gggatccccg ggtaccgagc tcgaatttcg ggcagcgttg
     5641 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
     5701 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
     5761 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
     5821 cgtaaagtct ggaaacgcgg aagtccccta cgtgctgctg aagttgcccg caacagagag
     5881 tggaaccaac cggtgatacc acgatactat gactgagagt caacgccatg agcggcctca
     5941 tttcttattc tgagttacaa cagtccgcac cgctgccggt agctccttcc ggtgggcgcg
     6001 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     6061 ggtgccggca gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     6121 acaagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg
     6181 tggaacacct acatctgtat taacgaagcg ctaaccgttt ttatcaggct ctgggaggca
     6241 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
     6301 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     6361 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     6421 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caaccaggcg
     6481 tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt
     6541 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     6601 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     6661 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     6721 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     6781 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     6841 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     6901 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg
     6961 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     7021 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     7081 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     7141 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     7201 ataactcaaa aa
//
