LOCUS       40924_46688        3085 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Regulator-reporter vector pWUR873, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     A growth- and bioluminescence-based bioreporter for the in vivo 
            detection of novel biocatalysts
  JOURNAL   Microb Biotechnol (2017) In press
  PUBMED    28393499
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Creutzburg SCA., Kengen SWM., van der Oost J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2016) Laboratory of Microbiology, Wageningen 
            University and Research, Stippeneng 4, Wageningen 6708 WE, The 
            Netherlands
REFERENCE   3  (bases 1 to 3085)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3085)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUL-2016) Laboratory of Microbiology, Wageningen University and 
            Research, Stippeneng 4, Wageningen 6708 WE, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        105..123
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             155..177
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             185..898
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      971..1018
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1225..2082)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2083..2174)
                     /label=AmpR promoter
     rep_origin      complement(2509..3054)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat
       61 ataagttgta attcggtacc ccgcttcggc ggggtttttt caagtaatac gactcactat
      121 agggagacca caacggtttc cctctagaaa taattttgtt taactataag aaggagatat
      181 acatatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      241 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      301 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      361 acttgtcact actttctctt atggtgttca atgcttttcc cgttatccgg atcacatgaa
      421 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc
      481 tttcaaagat gacgggaact acaagacgcg tgctgaagtc aagtttgaag gtgataccct
      541 tgttaatcgt atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttctcggaca
      601 caaactggag tacaactata actcacacaa tgtatacatc acggcagaca aacaaaagaa
      661 tggaatcaaa gctaacttca aaattcgcca caacattgaa gatggatccg ttcaactagc
      721 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      781 ttacctgtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg accacatggt
      841 ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaata
      901 aactagtgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      961 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1021 gaaaggagga actatatcta gtgcaagtgg cacttttcgg ggaaatgtgc gcggaacccc
     1081 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagat tatcaaaaag
     1141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2161 gaaaaataaa caaataggct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     2221 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact
     2281 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
     2341 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
     2401 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
     2461 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
     2521 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     2581 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc
     2641 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
     2701 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     2761 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac
     2821 atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag
     2881 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc
     2941 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag
     3001 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat
     3061 catcttatta atcagataaa atatt
//
