LOCUS       40924_46683       10294 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Regulator-reporter vector pWUR772, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     A growth- and bioluminescence-based bioreporter for the in vivo 
            detection of novel biocatalysts
  JOURNAL   Microb Biotechnol (2017) In press
  PUBMED    28393499
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Laboratory of Microbiology, Wageningen 
            University and Research, Stippeneng 4, Wageningen 6708 WE, The 
            Netherlands
REFERENCE   3  (bases 1 to 10294)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2016) Laboratory of Microbiology, Wageningen University and 
            Research, Stippeneng 4, Wageningen 6708 WE, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..87
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      95..100
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             107..982
                     /label=araC
                     /note="L-arabinose regulatory protein"
     CDS             complement(995..1807)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1814..1819)
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1828..2138)
                     /label=PBAD-adapt
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     rep_origin      2245..2790
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3060..3370
                     /label=PBAD-adapt
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     regulatory      3377..3382
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             3389..4828
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             4844..5764
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             5816..6895
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6913..7893
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             8075..9184
                     /label=LuxE
                     /note="LuxE"
     terminator      9207..9293
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9319..9413)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9437..10093)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(10094..10196)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctcgaggttg acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg
       61 gaagagagtc aattcagggt ggtgaatcgg accgaggagg atacgtatgg ctgaagcgca
      121 aaatgatccc ctgctgccgg gatactcgtt taatgcccat ctggtggcgg gtttaacgcc
      181 gattgaggcc aacggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta
      241 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt
      301 ttgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca
      361 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca
      421 tgaatggctt aactggccgt caatatttgc caatacgggg ttctttcgcc cggatgaagc
      481 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg
      541 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga
      601 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta
      661 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg
      721 cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag
      781 ctggcgcgag gaccaacgta tcagccaggc gaagctgctt ttgagcacca cccggatgcc
      841 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgggtatt
      901 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa
      961 tgatgtagcc gtcaagttgt cataacgatc gttagaaaaa ctcatcgagc atcaaatgaa
     1021 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     1081 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     1141 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     1201 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttgt
     1261 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     1321 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcggtcgc
     1381 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     1441 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     1501 cggggatcgc agtggtgagt aaccatgcgt catcaggagt acggataaaa tgcttgatgg
     1561 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     1621 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     1681 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     1741 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     1801 ggctcatacg tatcctcctc gccggcgccc aaaaaaacgg gtatggagaa acagtagaga
     1861 gttgcgataa aaagcgtcag gtaggatccg ctaatcttat ggataaaaat gctatgcgag
     1921 ctagcttcca tctcgactta ggttaatcaa tgtggacttt tctgccgtga ttatagacac
     1981 ttttgttacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg cttttaataa
     2041 gcggggttac cggtttggtt agcgagaaga gccagtaaaa gacgcagtga cggcaatgtc
     2101 tgatgcaata tggacaattg gtttcttctc tgaatggccc taggacaact tatatcgtat
     2161 ggggctgact tcaggtgcta catttgaaga gataaattgc actgaaatct agaaatattt
     2221 tatctgatta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc
     2281 tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc
     2341 tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta
     2401 gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg
     2461 ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg
     2521 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     2581 acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca
     2641 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc
     2701 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga
     2761 tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc
     2821 tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg
     2881 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc
     2941 tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac
     3001 ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg ctaggtaccg
     3061 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     3121 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     3181 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     3241 gtccacattg attaacctaa gtcgagatgg aactgcagtc gcatagcatt tttatccata
     3301 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     3361 tttttttggg gtcgacagga ggatacgtat gactaaaaaa atttcattca ttattaacgg
     3421 ccaggttgaa atctttcccg aaggtgatga tttagtgcaa tccattaatt ttggtgataa
     3481 tagtgtttac ctgccaatat tgaatgactc tcatgtaaaa aacattattg attgtaatgg
     3541 aaataacgaa ttacggttgc ataacattgt caattttctc tatacggtag ggcaaagatg
     3601 gaaaaatgaa gaatactcaa gacgcaggac atacattcgt gacttaaaaa aatatatggg
     3661 atattcagaa gaaatggcta agctagaggc caattggata tctatgattt tatgttctaa
     3721 aggcggcctt tatgatgttg tagaaaatga acttggttct cgccatatca tggatgaatg
     3781 gctacctcag gatgaaagtt atgttcgggc ttttccgaaa ggtaaatctg tacatctgtt
     3841 ggcaggtaat gttccattat ctgggatcat gtctatatta cgcgcaattt taactaagaa
     3901 tcagtgtatt ataaaaacat cgtcaaccga tccttttacc gctaatgcat tagcgttaag
     3961 ttttattgat gtagacccta atcatccgat aacgcgctct ttatctgtta tatattggcc
     4021 ccaccaaggt gatacatcac tcgcaaaaga aattatgcaa catgcggatg ttattgtcgc
     4081 ttggggaggg ccagatgcga ttaattgggc ggtagagcat gcgccatctt atgctgatgt
     4141 gattaaattt ggttctaaaa agagtctttg cattatcgat aatcctgttg atttgacgtc
     4201 cgcagcgaca ggtgcggctc atgatgtttg tttttacgat cagcgagctt gtttttctgc
     4261 ccaaaacata tattacatgg gaaatcatta tgaggaattt aagttagcgt tgatagaaaa
     4321 acttaatcta tatgcgcata tattaccgaa tgccaaaaaa gattttgatg aaaaggcggc
     4381 ctattcttta gttcaaaaag aaagcttgtt tgctggatta aaagtagagg tggatattca
     4441 tcaacgttgg atgattattg agtcaaatgc aggtgtggaa tttaatcaac cacttggcag
     4501 atgtgtgtac cttcatcacg tcgataatat tgagcaaata ttgccttatg ttcaaaaaaa
     4561 taagacgcaa accatatcta tttttccttg ggagtcatca tttaaatatc gagatgcgtt
     4621 agcattaaaa ggtgcggaaa ggattgtaga agcaggaatg aataacatat ttcgagttgg
     4681 tggatctcat gacggaatga gaccgttgca acgattagtg acatatattt ctcatgaaag
     4741 gccatctaac tatacggcta aggatgttgc ggttgaaata gaacagactc gattcctgga
     4801 agaagataag ttccttgtat ttgtcccata ataggtaaaa agtatggaaa atgaatcaaa
     4861 atataaaacc atcgaccacg ttatttgtgt tgaaggaaat aaaaaaattc atgtttggga
     4921 aacgctgcca gaagaaaaca gcccaaagag aaagaatgcc attattattg cgtctggttt
     4981 tgcccgcagg atggatcatt ttgctggtct ggcggaatat ttatcgcgga atggatttca
     5041 tgtgatccgc tatgattcgc ttcaccacgt tggattgagt tcagggacaa ttgatgaatt
     5101 tacaatgtct ataggaaagc agagcttgtt agcagtggtt gattggttaa ctacacgaaa
     5161 aataaataac ttcggtatgt tggcttcaag cttatctgcg cggatagctt atgcaagcct
     5221 atctgaaatc aatgcttcgt ttttaatcac cgcagtcggt gttgttaact taagatattc
     5281 tcttgaaaga gctttagggt ttgattatct cagtctaccc attaatgaat tgccgaataa
     5341 tctagatttt gaaggccata aattgggtgc tgaagtcttt gcgagagatt gtcttgattt
     5401 tggttgggaa gatttagctt ctacaattaa taacatgatg tatcttgata taccgtttat
     5461 tgcttttact gcaaataacg ataattgggt caagcaagat gaagttatca cattgttatc
     5521 aaatattcgt agtaatcgat gcaagatata ttctttgtta ggaagttcgc atgacttgag
     5581 tgaaaattta gtggtcctgc gcaattttta tcaatcggtt acgaaagccg ctatcgcgat
     5641 ggataatgat catctggata ttgatgttga tattactgaa ccgtcatttg aacatttaac
     5701 tattgcgaca gtcaatgaac gccgaatgag aattgagatt gaaaatcaag caatttctct
     5761 gtcttaaaat ctattgagat attctatcac tcaaatagca atataaggac tctctatgaa
     5821 atttggaaac tttttgctta cataccaacc tccccaattt tctcaaacag aggtaatgaa
     5881 acgtttggtt aaattaggtc gcatctctga ggagtgtggt tttgataccg tatggttact
     5941 ggagcatcat ttcacggagt ttggtttgct tggtaaccct tatgtcgctg ctgcatattt
     6001 acttggcgcg actaaaaaat tgaatgtagg aactgccgct attgttcttc ccacagccca
     6061 tccagtacgc caacttgaag atgtgaattt attggatcaa atgtcaaaag gacgatttcg
     6121 gtttggtatt tgccgagggc tttacaacaa ggactttcgc gtattcggca cagatatgaa
     6181 taacagtcgc gccttagcgg aatgctggta cgggctgata aagaatggca tgacagaggg
     6241 atatatggaa gctgataatg aacatatcaa gttccataag gtaaaagtaa accccgcggc
     6301 gtatagcaga ggtggcgcac cggtttatgt ggtggctgaa tcagcttcga cgactgagtg
     6361 ggctgctcaa tttggcctac cgatgatatt aagttggatt ataaatacta acgaaaagaa
     6421 agcacaactt gagctttata atgaagtggc tcaagaatat gggcacgata ttcataatat
     6481 cgaccattgc ttatcatata taacatctgt agatcatgac tcaattaaag cgaaagagat
     6541 ttgccggaaa tttctggggc attggtatga ttcttatgtg aatgctacga ctatttttga
     6601 tgattcagac caaacaagag gttatgattt caataaaggg cagtggcgtg actttgtatt
     6661 aaaaggacat aaagatacta atcgccgtat tgattacagt tacgaaatca atcccgtggg
     6721 aacgccgcag gaatgtattg acataattca aaaagacatt gatgctacag gaatatcaaa
     6781 tatttgttgt ggatttgaag ctaatggaac agtagacgaa attattgctt ccatgaagct
     6841 cttccagtct gatgtcatgc catttcttaa agaaaaacaa cgttcgctat tatattagct
     6901 aaggagaaag aaatgaaatt tggattgttc ttccttaact tcatcaattc aacaactgtt
     6961 caagaacaaa gtatagttcg catgcaggaa ataacggagt atgttgataa gttgaatttt
     7021 gaacagattt tagtgtatga aaatcatttt tcagataatg gtgttgtcgg cgctcctctg
     7081 actgtttctg gttttctgct cggtttaaca gagaaaatta aaattggttc attaaatcac
     7141 atcattacaa ctcatcatcc tgtccgcata gcggaggaag cttgcttatt ggatcagtta
     7201 agtgaaggga gatttatttt agggtttagt gattgcgaaa aaaaagatga aatgcatttt
     7261 tttaatcgcc cggttgaata tcaacagcaa ctatttgaag agtgttatga aatcattaac
     7321 gatgctttaa caacaggcta ttgtaatcca gataacgatt tttatagctt ccctaaaata
     7381 tctgtaaatc cccatgctta tacgccaggc ggacctcgga aatatgtaac agcaaccagt
     7441 catcatattg ttgagtgggc ggccaaaaaa ggtattcctc tcatctttaa gtgggatgat
     7501 tctaatgatg ttagatatga atatgctgaa agatataaag ccgttgcgga taaatatgac
     7561 gttgacctat cagagataga ccatcagtta atgatattag ttaactataa cgaagatagt
     7621 aataaagcta aacaagagac gcgtgcattt attagtgatt atgttcttga aatgcaccct
     7681 aatgaaaatt tcgaaaataa acttgaagaa ataattgcag aaaacgctgt cggaaattat
     7741 acggagtgta taactgcggc taagttggca attgaaaagt gtggtgcgaa aagtgtattg
     7801 ctgtcctttg aaccaatgaa tgatttgatg agccaaaaaa atgtaatcaa tattgttgat
     7861 gataatatta agaagtacca catggaatat acctaataga tttcgagttg cagcgaggcg
     7921 gcaagtgaac gaatccccag gagcatagat aactatgtga ctggggtgag tgaaagcagc
     7981 caacaaagca gcagcttgaa agatgaaggg tataaaagag tatgacagca gtgctgccat
     8041 actttctaat attatcttga ggagtaaaac aggtatgact tcatatgttg ataaacaaga
     8101 aattacagca agctcagaaa ttgatgattt gattttttcg agcgatccat tagtgtggtc
     8161 ttacgacgag caggaaaaaa tcagaaagaa acttgtgctt gatgcatttc gtaatcatta
     8221 taaacattgt cgagaatatc gtcactactg tcaggcacac aaagtagatg acaatattac
     8281 ggaaattgat gacatacctg tattcccaac atcggttttt aagtttactc gcttattaac
     8341 ttctcaggaa aacgagattg aaagttggtt taccagtagc ggcacgaatg gtttaaaaag
     8401 tcaggtggcg cgtgacagat taagtattga gagactctta ggctctgtga gttatggcat
     8461 gaaatatgtt ggtagttggt ttgatcatca aatagaatta gtcaatttgg gaccagatag
     8521 atttaatgct cataatattt ggtttaaata tgttatgagt ttggtggaat tgttatatcc
     8581 tacgacattt accgtaacag aagaacgaat agattttgtt aaaacattga atagtcttga
     8641 acgaataaaa aatcaaggga aagatctttg tcttattggt tcgccatact ttatttattt
     8701 actctgccat tatatgaaag ataaaaaaat ctcattttct ggagataaaa gcctttatat
     8761 cataaccgga ggcggctgga aaagttacga aaaagaatct ctgaaacgtg atgatttcaa
     8821 tcatctttta tttgatactt tcaatctcag tgatattagt cagatccgag atatatttaa
     8881 tcaagttgaa ctcaacactt gtttctttga ggatgaaatg cagcgtaaac atgttccgcc
     8941 gtgggtatat gcgcgagcgc ttgatcctga aacgttgaaa cctgtacctg atggaacgcc
     9001 ggggttgatg agttatatgg atgcgtcagc aaccagttat ccagcattta ttgttaccga
     9061 tgatgtcggg ataattagca gagaatatgg taagtatccc ggcgtgctcg ttgaaatttt
     9121 acgtcgcgtc aatacgagga cgcagaaagg gtgtgcttta agcttaaccg aagcgtttga
     9181 tagttgagcg gccgctctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     9241 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     9301 cctagaccta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     9361 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     9421 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     9481 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca
     9541 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     9601 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     9661 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     9721 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     9781 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     9841 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
     9901 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     9961 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
    10021 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
    10081 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
    10141 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
    10201 ctcattttcg ccagatatcg acgtctaaga aaccattatt atcatgacat taacctataa
    10261 aaataggcgt atcacgaggc cctttcgtct tcac
//
