LOCUS       40924_46673       10193 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Regulator-reporter vector pWUR768, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     A growth- and bioluminescence-based bioreporter for the in vivo 
            detection of novel biocatalysts
  JOURNAL   Microb Biotechnol (2017) In press
  PUBMED    28393499
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Laboratory of Microbiology, Wageningen 
            University and Research, Stippeneng 4, Wageningen 6708 WE, The 
            Netherlands
REFERENCE   3  (bases 1 to 10193)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10193)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2016) Laboratory of Microbiology, Wageningen University and 
            Research, Stippeneng 4, Wageningen 6708 WE, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..87
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      95..100
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             107..982
                     /label=araC
                     /note="L-arabinose regulatory protein"
     CDS             complement(995..1807)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1814..1819)
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1828..2138)
                     /label=PBAD-adapt
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     rep_origin      complement(2289..2877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2959..3269
                     /label=PBAD-adapt
                     /note="PBAD-adapt"
                     /regulatory_class="promoter"
     regulatory      3276..3281
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             3288..4727
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             4743..5663
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             5715..6794
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6812..7792
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             7974..9083
                     /label=LuxE
                     /note="LuxE"
     terminator      9106..9192
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9218..9312)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9336..9992)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9993..10095)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctcgaggttg acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg
       61 gaagagagtc aattcagggt ggtgaatcgg accgaggagg atacgtatgg ctgaagcgca
      121 aaatgatccc ctgctgccgg gatactcgtt taatgcccat ctggtggcgg gtttaacgcc
      181 gattgaggcc aacggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta
      241 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt
      301 ttgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca
      361 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca
      421 tgaatggctt aactggccgt caatatttgc caatacgggg ttctttcgcc cggatgaagc
      481 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg
      541 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga
      601 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta
      661 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg
      721 cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag
      781 ctggcgcgag gaccaacgta tcagccaggc gaagctgctt ttgagcacca cccggatgcc
      841 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgggtatt
      901 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa
      961 tgatgtagcc gtcaagttgt cataacgatc gttagaaaaa ctcatcgagc atcaaatgaa
     1021 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     1081 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     1141 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     1201 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttgt
     1261 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     1321 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcggtcgc
     1381 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     1441 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     1501 cggggatcgc agtggtgagt aaccatgcgt catcaggagt acggataaaa tgcttgatgg
     1561 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     1621 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     1681 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     1741 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     1801 ggctcatacg tatcctcctc gccggcgccc aaaaaaacgg gtatggagaa acagtagaga
     1861 gttgcgataa aaagcgtcag gtaggatccg ctaatcttat ggataaaaat gctatgcgag
     1921 ctagcttcca tctcgactta ggttaatcaa tgtggacttt tctgccgtga ttatagacac
     1981 ttttgttacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg cttttaataa
     2041 gcggggttac cggtttggtt agcgagaaga gccagtaaaa gacgcagtga cggcaatgtc
     2101 tgatgcaata tggacaattg gtttcttctc tgaatggccc taggcgttcg gctgcggcga
     2161 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2221 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2281 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2341 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2401 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2461 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     2521 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2581 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2641 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2701 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     2761 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2821 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2881 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2941 attttggtca tgggtaccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca
     3001 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat
     3061 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt
     3121 ctataatcac ggcagaaaag tccacattga ttaacctaag tcgagatgga actgcagtcg
     3181 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
     3241 tactgtttct ccatacccgt ttttttgggg tcgacaggag gatacgtatg actaaaaaaa
     3301 tttcattcat tattaacggc caggttgaaa tctttcccga aggtgatgat ttagtgcaat
     3361 ccattaattt tggtgataat agtgtttacc tgccaatatt gaatgactct catgtaaaaa
     3421 acattattga ttgtaatgga aataacgaat tacggttgca taacattgtc aattttctct
     3481 atacggtagg gcaaagatgg aaaaatgaag aatactcaag acgcaggaca tacattcgtg
     3541 acttaaaaaa atatatggga tattcagaag aaatggctaa gctagaggcc aattggatat
     3601 ctatgatttt atgttctaaa ggcggccttt atgatgttgt agaaaatgaa cttggttctc
     3661 gccatatcat ggatgaatgg ctacctcagg atgaaagtta tgttcgggct tttccgaaag
     3721 gtaaatctgt acatctgttg gcaggtaatg ttccattatc tgggatcatg tctatattac
     3781 gcgcaatttt aactaagaat cagtgtatta taaaaacatc gtcaaccgat ccttttaccg
     3841 ctaatgcatt agcgttaagt tttattgatg tagaccctaa tcatccgata acgcgctctt
     3901 tatctgttat atattggccc caccaaggtg atacatcact cgcaaaagaa attatgcaac
     3961 atgcggatgt tattgtcgct tggggagggc cagatgcgat taattgggcg gtagagcatg
     4021 cgccatctta tgctgatgtg attaaatttg gttctaaaaa gagtctttgc attatcgata
     4081 atcctgttga tttgacgtcc gcagcgacag gtgcggctca tgatgtttgt ttttacgatc
     4141 agcgagcttg tttttctgcc caaaacatat attacatggg aaatcattat gaggaattta
     4201 agttagcgtt gatagaaaaa cttaatctat atgcgcatat attaccgaat gccaaaaaag
     4261 attttgatga aaaggcggcc tattctttag ttcaaaaaga aagcttgttt gctggattaa
     4321 aagtagaggt ggatattcat caacgttgga tgattattga gtcaaatgca ggtgtggaat
     4381 ttaatcaacc acttggcaga tgtgtgtacc ttcatcacgt cgataatatt gagcaaatat
     4441 tgccttatgt tcaaaaaaat aagacgcaaa ccatatctat ttttccttgg gagtcatcat
     4501 ttaaatatcg agatgcgtta gcattaaaag gtgcggaaag gattgtagaa gcaggaatga
     4561 ataacatatt tcgagttggt ggatctcatg acggaatgag accgttgcaa cgattagtga
     4621 catatatttc tcatgaaagg ccatctaact atacggctaa ggatgttgcg gttgaaatag
     4681 aacagactcg attcctggaa gaagataagt tccttgtatt tgtcccataa taggtaaaaa
     4741 gtatggaaaa tgaatcaaaa tataaaacca tcgaccacgt tatttgtgtt gaaggaaata
     4801 aaaaaattca tgtttgggaa acgctgccag aagaaaacag cccaaagaga aagaatgcca
     4861 ttattattgc gtctggtttt gcccgcagga tggatcattt tgctggtctg gcggaatatt
     4921 tatcgcggaa tggatttcat gtgatccgct atgattcgct tcaccacgtt ggattgagtt
     4981 cagggacaat tgatgaattt acaatgtcta taggaaagca gagcttgtta gcagtggttg
     5041 attggttaac tacacgaaaa ataaataact tcggtatgtt ggcttcaagc ttatctgcgc
     5101 ggatagctta tgcaagccta tctgaaatca atgcttcgtt tttaatcacc gcagtcggtg
     5161 ttgttaactt aagatattct cttgaaagag ctttagggtt tgattatctc agtctaccca
     5221 ttaatgaatt gccgaataat ctagattttg aaggccataa attgggtgct gaagtctttg
     5281 cgagagattg tcttgatttt ggttgggaag atttagcttc tacaattaat aacatgatgt
     5341 atcttgatat accgtttatt gcttttactg caaataacga taattgggtc aagcaagatg
     5401 aagttatcac attgttatca aatattcgta gtaatcgatg caagatatat tctttgttag
     5461 gaagttcgca tgacttgagt gaaaatttag tggtcctgcg caatttttat caatcggtta
     5521 cgaaagccgc tatcgcgatg gataatgatc atctggatat tgatgttgat attactgaac
     5581 cgtcatttga acatttaact attgcgacag tcaatgaacg ccgaatgaga attgagattg
     5641 aaaatcaagc aatttctctg tcttaaaatc tattgagata ttctatcact caaatagcaa
     5701 tataaggact ctctatgaaa tttggaaact ttttgcttac ataccaacct ccccaatttt
     5761 ctcaaacaga ggtaatgaaa cgtttggtta aattaggtcg catctctgag gagtgtggtt
     5821 ttgataccgt atggttactg gagcatcatt tcacggagtt tggtttgctt ggtaaccctt
     5881 atgtcgctgc tgcatattta cttggcgcga ctaaaaaatt gaatgtagga actgccgcta
     5941 ttgttcttcc cacagcccat ccagtacgcc aacttgaaga tgtgaattta ttggatcaaa
     6001 tgtcaaaagg acgatttcgg tttggtattt gccgagggct ttacaacaag gactttcgcg
     6061 tattcggcac agatatgaat aacagtcgcg ccttagcgga atgctggtac gggctgataa
     6121 agaatggcat gacagaggga tatatggaag ctgataatga acatatcaag ttccataagg
     6181 taaaagtaaa ccccgcggcg tatagcagag gtggcgcacc ggtttatgtg gtggctgaat
     6241 cagcttcgac gactgagtgg gctgctcaat ttggcctacc gatgatatta agttggatta
     6301 taaatactaa cgaaaagaaa gcacaacttg agctttataa tgaagtggct caagaatatg
     6361 ggcacgatat tcataatatc gaccattgct tatcatatat aacatctgta gatcatgact
     6421 caattaaagc gaaagagatt tgccggaaat ttctggggca ttggtatgat tcttatgtga
     6481 atgctacgac tatttttgat gattcagacc aaacaagagg ttatgatttc aataaagggc
     6541 agtggcgtga ctttgtatta aaaggacata aagatactaa tcgccgtatt gattacagtt
     6601 acgaaatcaa tcccgtggga acgccgcagg aatgtattga cataattcaa aaagacattg
     6661 atgctacagg aatatcaaat atttgttgtg gatttgaagc taatggaaca gtagacgaaa
     6721 ttattgcttc catgaagctc ttccagtctg atgtcatgcc atttcttaaa gaaaaacaac
     6781 gttcgctatt atattagcta aggagaaaga aatgaaattt ggattgttct tccttaactt
     6841 catcaattca acaactgttc aagaacaaag tatagttcgc atgcaggaaa taacggagta
     6901 tgttgataag ttgaattttg aacagatttt agtgtatgaa aatcattttt cagataatgg
     6961 tgttgtcggc gctcctctga ctgtttctgg ttttctgctc ggtttaacag agaaaattaa
     7021 aattggttca ttaaatcaca tcattacaac tcatcatcct gtccgcatag cggaggaagc
     7081 ttgcttattg gatcagttaa gtgaagggag atttatttta gggtttagtg attgcgaaaa
     7141 aaaagatgaa atgcattttt ttaatcgccc ggttgaatat caacagcaac tatttgaaga
     7201 gtgttatgaa atcattaacg atgctttaac aacaggctat tgtaatccag ataacgattt
     7261 ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg gacctcggaa
     7321 atatgtaaca gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag gtattcctct
     7381 catctttaag tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa gatataaagc
     7441 cgttgcggat aaatatgacg ttgacctatc agagatagac catcagttaa tgatattagt
     7501 taactataac gaagatagta ataaagctaa acaagagacg cgtgcattta ttagtgatta
     7561 tgttcttgaa atgcacccta atgaaaattt cgaaaataaa cttgaagaaa taattgcaga
     7621 aaacgctgtc ggaaattata cggagtgtat aactgcggct aagttggcaa ttgaaaagtg
     7681 tggtgcgaaa agtgtattgc tgtcctttga accaatgaat gatttgatga gccaaaaaaa
     7741 tgtaatcaat attgttgatg ataatattaa gaagtaccac atggaatata cctaatagat
     7801 ttcgagttgc agcgaggcgg caagtgaacg aatccccagg agcatagata actatgtgac
     7861 tggggtgagt gaaagcagcc aacaaagcag cagcttgaaa gatgaagggt ataaaagagt
     7921 atgacagcag tgctgccata ctttctaata ttatcttgag gagtaaaaca ggtatgactt
     7981 catatgttga taaacaagaa attacagcaa gctcagaaat tgatgatttg attttttcga
     8041 gcgatccatt agtgtggtct tacgacgagc aggaaaaaat cagaaagaaa cttgtgcttg
     8101 atgcatttcg taatcattat aaacattgtc gagaatatcg tcactactgt caggcacaca
     8161 aagtagatga caatattacg gaaattgatg acatacctgt attcccaaca tcggttttta
     8221 agtttactcg cttattaact tctcaggaaa acgagattga aagttggttt accagtagcg
     8281 gcacgaatgg tttaaaaagt caggtggcgc gtgacagatt aagtattgag agactcttag
     8341 gctctgtgag ttatggcatg aaatatgttg gtagttggtt tgatcatcaa atagaattag
     8401 tcaatttggg accagataga tttaatgctc ataatatttg gtttaaatat gttatgagtt
     8461 tggtggaatt gttatatcct acgacattta ccgtaacaga agaacgaata gattttgtta
     8521 aaacattgaa tagtcttgaa cgaataaaaa atcaagggaa agatctttgt cttattggtt
     8581 cgccatactt tatttattta ctctgccatt atatgaaaga taaaaaaatc tcattttctg
     8641 gagataaaag cctttatatc ataaccggag gcggctggaa aagttacgaa aaagaatctc
     8701 tgaaacgtga tgatttcaat catcttttat ttgatacttt caatctcagt gatattagtc
     8761 agatccgaga tatatttaat caagttgaac tcaacacttg tttctttgag gatgaaatgc
     8821 agcgtaaaca tgttccgccg tgggtatatg cgcgagcgct tgatcctgaa acgttgaaac
     8881 ctgtacctga tggaacgccg gggttgatga gttatatgga tgcgtcagca accagttatc
     8941 cagcatttat tgttaccgat gatgtcggga taattagcag agaatatggt aagtatcccg
     9001 gcgtgctcgt tgaaatttta cgtcgcgtca atacgaggac gcagaaaggg tgtgctttaa
     9061 gcttaaccga agcgtttgat agttgagcgg ccgctctaga ggcatcaaat aaaacgaaag
     9121 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     9181 agtaggacaa atccgccgcc ctagacctag tgcttggatt ctcaccaata aaaaacgccc
     9241 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     9301 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     9361 tactgttgta attcattaag cattctgccg acatggaagc catcacagac ggcatgatga
     9421 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     9481 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     9541 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     9601 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     9661 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     9721 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
     9781 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     9841 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     9901 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     9961 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
    10021 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
    10081 cttacgtgcc gatcaacgtc tcattttcgc cagatatcga cgtctaagaa accattatta
    10141 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//
