LOCUS       40924_46633       13103 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pWSMV-S1RN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Stenger DC, French R, Choi I-R.
  TITLE     pWSMV-S1RN: an improved plant virus vector to express foreign genes 
            in cereals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Stenger DC, French R, Choi I-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2000) USDA-ARS, University of Nebraska-Lincoln, 
            344 Keim Hall, Lincoln, NE 68583, USA
REFERENCE   3  (bases 1 to 13103)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2000) USDA-ARS, University of Nebraska-Lincoln, 344 Keim 
            Hall, Lincoln, NE 68583, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..9492
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31741"
                     /organism="Wheat streak mosaic virus"
     CDS             131..9241
                     /codon_start=1
                     /product="polyprotein"
                     /label=polyprotein
                     /protein_id="AAK16492.1"
                     /translation="MATANCLLGDFGRQGVVANDPYVQCRARTLIFLSTEEEVDVVVNH
                     HGPGSIFWSKEGILTQTAKNLYKATAYGLGYDLAANVFVCGKCRSSCTQYRYFIEDHFA
                     CDKLVEKNCAYIKDDKYVKVVEAFPIMPSYATPGQETRIIQWMNKTSQCLADHCIQRTR
                     EITFTNSKTQEEETRVKDCSLEVFYDDFDEAHAVIEHAHRKNPVHEYKEKQLRMTSNNI
                     AALVDQVTRMMHSKGKTVEIVGSKGHKKFAKIPLKHTMGYPKRDWDATKDIPEDFRGFI
                     TTYSGVIQYTRKVQDHEVTLGWSGVLLSEMDVPDGYQEDCVDGLFIVMGRCAHGRIQNA
                     LKPKCTHGLRWYGVDQAVNKVIPKYHDVCNNNCASYLEALPRKVSRVWQSMFDIHNLRC
                     DQCRTEWKMRTASEHLQLLQKSVEHYMQTYPDSDVTLFKAFLQALGPDEEVEARQLKPN
                     NTLQLVDVWRTMKNTINIPNRIIYMGMFTDNYGNFDFFPNTSMPELFAMHMQPVQHKML
                     EDGTIETNFRFVDLEGKIQTSIESLYPTFDSTYWNEQARRVHRIQPLADCSMEINGESK
                     PICHWEGNVPLYNPIIRATPGQLPFGVTTHLLSVNDRSGRFHYVPKNGYCYMYIFACAM
                     IFCGNSNRSTVDAFVRQVCEDLGPWPMFGDVLRQLDWMATFYGCYDALVPVILVDHTRK
                     TMHVPTPYGIKQSGMHTIRVNTVLELITLDTMASGAMKDYKIGGFQETVLSIQACVKSR
                     KEFVRKINKDAEWLVDMFINPSTLFVLGGLIEVHQVILADVENSFDKSAALLNLRQIAL
                     KLGPHLESKQRVRQYMELMIQHRASVEAIIPSQHMKAEMMQYIDALQRSILEEQVIIEM
                     DRVGGKEKMLVEQDLSHAECAYNEFFNSIGYLNFHGTVLRLTYSGPGRKVGEVLESLRD
                     NWLTRYLRGPKQPRDYKGSSLRIWRKVTHLCGNAYRWVFYNMAANVLQVILIGLSTVFG
                     AYLLKKILKMLQWEKEQESTELVEYQGKREEAWITRVMAVLYIIASLFSVDFSSALYSN
                     LVKFRTIFDILKFNCEYQSGIFESLKNQLGNIPAFHEVHLYDHEATQVAVPPAILTFER
                     WFETRITSGQQGYAPLDGSHVSLTMTKDTVGEIATQVQTHKAKEFLIIGHVGCGKSTAF
                     PATLSRNGRVMICEPTRVLVTNLQDSMLATRNLSISAMMRNHRVMTASNITVTTYGYAL
                     HYLYNNSHNLSEYDYILFDEVHQTSAEMLVFYNWLKSTTWEGKLIKLTATNNTVNGDMQ
                     TQQALDVKTWPVMDHRTFMQEQGRGTAHDASTLGDVIIVFLTSFREIDESADILSKNSK
                     IGVIKADSRHLRNKISLMDDVEALRAEKKYILATNILQNGVNLHADVVVDFGFKIVPAI
                     DSDNRMITVKRQLINKSDRIQRLGRVGRMKMGYARKIGNEIDASFALDEVTATEAALLA
                     FGLGVAPVLQNVDQHTFGKITAEQVRTAARFEMQLSYMVWMINRDGTMATRLYEQFKSL
                     LLTPGNTSLAPYYETLVDTHRFRTIGQYATLGYMRTDEKHHLVLPFHHNDVSVEFAERI
                     GEAYMASQVPTSIKLRVPAVNHREVAMKMSANPEDVGTILYMVEQALISEKTKLENLTQ
                     AFQQQQSTYCSVLIPNFNVAGRLTQAMDRIRKNVSVLQHQKTALEKAAVTYDYTKLVEL
                     LDENPSIASHVSYQAGPAKFIDEFILEKRDYGWLPYLAVGTACAIAGTTLVMMYYRRMK
                     RSVKFEGKAARNRSAKRQSARDQKMERGNEYTYYDAGDTLYNGVQENMNHAPDWTDRIK
                     KKTHAYAMQFGREVPKTETQRSSQYWHFYGFDPKMYDSVEFKDIAANFSVHQDAKAMDL
                     QKAFTEMVENRWDDEDFFDEKIPKRVLAIFRKGDKVREVALAPHKPNQVNKRGLPVGHA
                     DHRGEWRQTQPSFEKEVSYENKSTFEGARSLDHIHQNQVILVEDNQQLNGLIVGNILLA
                     PYHFTRGMRNREEKETRMLTQFGTYNLGKLTNKHVTKFTMMDLVALTLPPTFQARRKLK
                     CFRPPREGERAMLVTMQYEKAGWVAKQSAETTITPFGDRHDGLWKHRISTGPGDCGSAI
                     VAVADLKVVGFHNLGGKGENYFTPITIEVMDFLAEKSVTPLVPWKFSDEQVDLCGLIAA
                     NGADKYPFTKTISDLVSWQSLQMTKYCGENFKAIAYAPNRMSKRHVITGKRPEFIKFLD
                     SHPKWNATVTPFLNGFQPSVLTHEAYYKDVLKYNKDIIVGGTDEVCFAKAVVATIGILE
                     IAGFSKGQFQPIFDGCKIFNDLNLDAAMGALYSGKKSAYFDGATSDEINEFFELSAAKL
                     LSNGHGVWSGLLKAELRPKAKVVANKTRTFTSAPIDILMGAKAVVDEFNKFFYTKHLRG
                     PWTVGINKFNGGWDLLAKNLMVHEWFIDADGSQFDSSITPLLMNAILNIRQYFMAEDDE
                     AEQMLANLYTQIINTCILIEDGTIVQKFRGNNSGQPSTVVDNTMCLIIAMEYCRMRVEK
                     DHEHRMRILYVCNGDDLLINADTKDKDFIQQYFADYMRELELNYSFDEAYRSIEEVEYM
                     SHTFMKRNSMYIPKLKRERIVAILEWQRSKEPKAIQSAIIAAYVEAFGYDEFTEMIEEL
                     AQEVSAVWPDFKLPSRQEVEDLYLTGTRTDLGEEIKECGEQYCVYESSEAATDAVLAAA
                     NAGTGSASSSGSTQSSQSASTASGSGSSPSGSGSGAAGGSGSGSAQTQSNNVSVMAGLD
                     TGGAKTDQGSGSKGTGGSFTSNPVRTGGRATDVQDQTPGLVFPAPKITTKAIYMPKTVR
                     DKIKPEMINNMIKYQPRAELIDNRYATTEQLNTWIKEASEGLDVTEDVFINTLLPGWVY
                     HCIINTTSPENRALGTWRVVNNAGKDNEQQLEFKIEPMYKAAKPSLRAIMRHFGEGARV
                     MIEESVRIGKPIIPRGFDKAGVLSINNIVAACDFIMRGADDTPNFVQVQNSVAVNRLRG
                     IQNKLFAQARLSAGTNEDNSRHDADDVRENTHSFNGVNALA"
     misc_feature    1190..1195
                     /label=unique SalI site
                     /note="unique SalI site"
     rep_origin      complement(9766..10311)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(10479..11336)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11337..11441)
                     /label=AmpR promoter
     CDS             complement(12300..13082)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 aaattaaacc aacccaaatc gatctgacaa cgaacaaaac gaactcaaag cacccacaag
       61 ctctagttca acaaagttca tcacgcagaa agcaattcgt tcattgtgag ctctcgcata
      121 gagataagca atggcaacag cgaattgttt gctcggtgac ttcggaaggc agggggtcgt
      181 cgctaacgac ccatatgtac agtgcagagc gcgcacgctg attttcctaa gcactgagga
      241 ggaggttgat gtggtggtta accaccacgg accaggaagc attttctggt caaaagaagg
      301 tatcttaaca cagacggcta agaaccttta caaagctact gcgtatggct taggctacga
      361 ccttgcagca aacgtcttcg tttgcggcaa gtgtaggtcc agctgcactc agtataggta
      421 cttcattgag gatcactttg cttgcgacaa actcgtggag aagaactgcg cgtacatcaa
      481 ggatgataag tacgtgaagg ttgtggaggc attcccaatt atgccatctt atgccacacc
      541 agggcaagaa acacgcatca tacagtggat gaacaagaca agtcaatgcc ttgctgatca
      601 ctgcatccaa cgcactcgcg agatcacttt caccaactca aaaactcagg aggaagaaac
      661 tcgcgtgaag gattgcagtt tggaagtatt ttatgatgac ttcgatgaag ctcatgctgt
      721 gattgagcac gcacatcgga aaaatccggt tcacgaatat aaggagaagc agctgcggat
      781 gacttcaaat aatatagctg cgttagtgga ccaagtgaca cggatgatgc actccaaggg
      841 caaaacagtg gaaatagttg ggagtaaagg ccataagaaa tttgccaaaa ttccactgaa
      901 gcacacaatg ggatatccaa agcgggattg ggacgcaaca aaggacatac cagaagattt
      961 cagaggcttt atcacaactt atagtggcgt catacaatac acacgcaaag tgcaggatca
     1021 cgaagtgacg cttggatgga gtggtgttct gcttagtgag atggatgttc ctgatggcta
     1081 tcaagaagat tgcgttgacg gtctatttat tgtcatggga agatgtgcac atggacgcat
     1141 tcaaaatgca ctgaagccga aatgcacaca tggacttaga tggtatggcg tcgaccaagc
     1201 agtgaacaaa gttattccga aatatcacga tgtgtgcaac aacaactgcg caagttacct
     1261 ggaagccttg ccaagaaagg ttagccgtgt atggcaatca atgtttgaca tacataatct
     1321 gaggtgcgac caatgtagaa cagagtggaa gatgcgaaca gcctcagagc atttgcaatt
     1381 gttacagaaa tcggtggaac attacatgca aacgtatcca gacagcgatg taacactgtt
     1441 caaagcattc ttgcaagcac tagggccaga tgaagaggtt gaggcacgcc agctcaaacc
     1501 caacaataca ttgcaacttg tagatgtgtg gcgaacaatg aaaaacacaa taaacatccc
     1561 gaatagaatc atctacatgg gcatgttcac ggacaattat gggaattttg atttctttcc
     1621 gaacacatca atgcctgaat tattcgccat gcacatgcaa ccggtgcaac ataagatgct
     1681 agaagatgga acaattgaaa cgaattttag gttcgttgat ttggaaggga aaatccaaac
     1741 tagcattgaa agtttgtatc cgacatttga cagcacatac tggaatgaac aagcacgcag
     1801 agttcacaga atccaaccgc tagcagactg ctcgatggaa attaacggcg agtctaagcc
     1861 catttgtcac tgggaaggaa atgtgccttt gtacaatccc attattcgtg caacgcctgg
     1921 acaattaccg tttggagtta caacgcattt gctgagtgta aatgacagga gtggaagatt
     1981 ccattatgtg ccgaagaatg ggtattgtta catgtacata tttgcctgtg cgatgatttt
     2041 ctgtggtaat agcaatcgtt ccacagttga cgcctttgtt cggcaagtct gcgaggatct
     2101 gggtccttgg ccaatgtttg gtgatgtact aagacaactt gactggatgg cgacctttta
     2161 tggatgttac gacgcattgg tgccagtcat cttggtagac catacaagaa agaccatgca
     2221 cgtgccaaca ccatatggta taaagcaatc aggaatgcat accatcagag tcaacacagt
     2281 gctagagttg ataactctcg ataccatggc cagtggagca atgaaagatt acaaaattgg
     2341 cgggttccaa gagactgttc tcagtataca agcatgcgtc aagagcagaa aagaatttgt
     2401 gcgcaaaatt aacaaggatg cagaatggtt ggtggatatg tttattaatc cttcaacgct
     2461 cttcgtgttg ggaggcttaa ttgaagtgca ccaagtcatt cttgctgatg tcgaaaattc
     2521 attcgacaaa tcagcagctt tactaaactt gcgccaaata gctttaaagc tcggaccaca
     2581 cttggaatca aagcagcgtg tacgtcagta catggagtta atgattcagc atcgggcatc
     2641 agttgaagca ataattccct cgcaacacat gaaagctgag atgatgcaat atattgatgc
     2701 actgcaacgc tcaattcttg aggagcaagt aatcatagag atggatcgag ttggaggaaa
     2761 ggaaaaaatg ctcgtcgagc aagatctttc acacgcagag tgtgcgtaca acgagttctt
     2821 caactccatt ggctacttaa actttcatgg aaccgtttta cgactcacat attctggtcc
     2881 aggaagaaag gttggagaag tgctagagag tttaagagac aactggttga cacgctatct
     2941 ccgaggaccg aagcagccga gagactacaa agggagttcc ttgaggattt ggaggaaggt
     3001 tactcacctt tgcggaaacg cttacaggtg ggtattttac aacatggccg cgaacgtctt
     3061 gcaagttata ctcataggcc tttctaccgt tttcggagca tatttattaa agaagatctt
     3121 aaaaatgctg cagtgggaga aggagcaaga aagcacagaa ttggttgaat accaagggaa
     3181 gcgagaggaa gcatggataa cacgagtaat ggctgtattg tatataatag cttcactttt
     3241 ctccgtagat tttagttctg ctttatattc gaatttggtg aagttcagaa ccatatttga
     3301 catcttgaag tttaattgcg aatatcagag tggaattttt gaaagtctga aaaatcaact
     3361 cggcaacatc ccagcgtttc atgaggtaca tttgtatgac catgaggcaa cacaagtagc
     3421 agttccacca gcgatattga cattcgagcg atggttcgag actcggatca catctggcca
     3481 gcaaggatat gcaccacttg atggtagcca cgtgagctta acgatgacaa aagacacagt
     3541 tggtgagatc gcaacacaag tgcaaacgca caaggcaaag gagtttctta ttataggaca
     3601 tgttggatgt gggaaatcta cagcttttcc agcaacactc tcccggaatg ggcgtgtgat
     3661 gatatgcgaa ccaacccgcg tgctagtcac aaatttgcag gattcaatgc tagcaacgag
     3721 aaatctaagc atcagtgcca tgatgagaaa ccaccgggtt atgacggcgt cgaatataac
     3781 agtaactacg tatgggtatg cactccacta cttgtacaac aactcacata acctttcaga
     3841 atatgattat attctttttg acgaagtgca ccaaacctcg gcagagatgt tagtgtttta
     3901 caactggctt aagagcacaa cgtgggaggg caagctcatc aagttaacag ccacgaacaa
     3961 cacagttaat ggcgacatgc aaacacagca agccctcgat gtcaaaacgt ggccggttat
     4021 ggatcacaga acattcatgc aggagcaagg tcgaggaacg gctcacgatg catcaactct
     4081 tggtgatgtc atcatagtct tcttaacttc gtttcgtgaa attgatgaat ctgctgatat
     4141 tttaagcaaa aattcaaaga ttggcgtaat aaaggcagat agtcggcact tgcgaaacaa
     4201 aattagcttg atggatgatg tcgaagcact ccgagctgag aagaaataca ttcttgcaac
     4261 taatattctt caaaacggtg tgaatttgca tgcagacgtt gtcgttgatt ttggcttcaa
     4321 gattgtacca gccattgaca gcgacaatcg gatgatcaca gtcaagcgcc aattgatcaa
     4381 caaatcagac aggattcaga gactaggacg agtaggccgg atgaagatgg gatacgcaag
     4441 gaaaatcgga aatgaaatag atgcctcatt cgctttggat gaagtcacag ctacggaagc
     4501 tgccttgttg gcatttggac tcggtgttgc tccagtgttg cagaatgtcg atcaacacac
     4561 attcggaaaa ataacagcag agcaagttcg aacagctgcg cgctttgaaa tgcaattgtc
     4621 gtatatggtg tggatgatta acagagatgg cactatggcc acacggttat atgaacaatt
     4681 caaatcattg cttttaacac cagggaacac gagtttggct ccgtattacg agacactcgt
     4741 tgacactcac agattcagaa ctattggaca atacgcaact cttggataca tgcgcacaga
     4801 cgaaaagcac catttggtat taccatttca tcataatgac gtgagcgttg aatttgccga
     4861 aaggattggc gaagcctata tggcctctca agtcccgaca tcaatcaagt tgcgcgtacc
     4921 tgctgtaaat catagagaag tggcgatgaa gatgtcagca aatcctgaag atgtgggaac
     4981 catattgtat atggttgagc aagcgctaat aagtgaaaag acgaaactgg agaatttaac
     5041 ccaggcattc cagcagcaac aatcaacata ttgcagcgtg ctcatcccta attttaatgt
     5101 tgctggtcgg ctaacacagg caatggatcg cataagaaag aatgtttctg tgctgcaaca
     5161 ccagaaaaca gctctcgaaa aggcggcagt gacttacgac tacacgaagt tagttgagct
     5221 ccttgatgaa aatccaagca tagcttccca tgtgtcatac caggctgggc cagcaaaatt
     5281 cattgatgaa ttcatattgg agaagcgcga ttatgggtgg ctaccatatc ttgcagtagg
     5341 aactgcgtgt gcaattgctg gcacaacact tgtaatgatg tactaccgtc gcatgaagcg
     5401 tagtgtcaag ttcgagggca aagcagcacg caacaggagt gcaaaacgac aatcagcaag
     5461 agaccaaaag atggagcgtg gtaacgaata cacatactac gatgctggtg acaccttgta
     5521 taatggagtt caagagaata tgaatcatgc accagactgg accgatcgga ttaagaagaa
     5581 gactcatgca tacgctatgc aatttggtag ggaagtacca aagactgaaa cacagcgatc
     5641 ctcacaatac tggcacttct acggttttga tccaaagatg tatgactcag tcgaattcaa
     5701 ggacatagca gcaaacttct cagtgcacca ggatgcaaag gcaatggatt tgcagaaagc
     5761 cttcacagaa atggtggaaa atcgttggga tgatgaagac ttcttcgacg agaagatacc
     5821 aaagcgagtt ttggccatct tcaggaaagg agacaaggtt cgtgaagttg cattggcacc
     5881 tcacaagcca aaccaagtca acaagcgtgg gctacctgtc ggacatgctg atcacagagg
     5941 agagtggaga caaacacagc cttcatttga aaaagaagtg tcgtacgaga acaaatcaac
     6001 tttcgaaggt gcacgttcac ttgatcatat ccatcagaat caagtcatcc tcgttgaaga
     6061 caatcagcag ttaaatgggc taatagttgg gaacatactc ttggcgccat atcatttcac
     6121 acgaggtatg aggaacagag aggagaagga gacacgcatg ttgacacagt ttggaacgta
     6181 caatcttgga aaacttacca acaagcatgt cacaaaattt acaatgatgg atctggtagc
     6241 attaaccttg cctccaacat ttcaagcaag acggaaactc aaatgtttca gaccaccaag
     6301 ggaaggagag cgagcaatgt tggtgaccat gcagtacgag aaagcaggat gggttgccaa
     6361 gcaatcagca gaaacaacaa tcacaccatt cggtgatcga catgatggtt tgtggaagca
     6421 tagaatttca acaggaccag gtgactgtgg aagcgccata gtagcagtag cagacctaaa
     6481 agttgtggga ttccataacc ttggagggaa aggtgagaat tatttcacac cgataactat
     6541 tgaggtcatg gatttcttag ctgaaaagtc tgtgacaccg cttgtgccat ggaagttctc
     6601 agacgagcaa gttgacttat gtggtttaat tgcggccaat ggagcagaca aatacccatt
     6661 caccaaaaca ataagcgact tggttagttg gcaaagtctc caaatgacga aatactgtgg
     6721 ggagaacttc aaggctatcg cttatgctcc aaaccgaatg tcgaaaaggc atgtcataac
     6781 aggaaagagg cctgaattca ttaaatttct agattcccac ccgaagtgga atgcaacggt
     6841 aacacctttc ttaaacgggt ttcaaccatc agttttgaca catgaagcat attacaagga
     6901 tgtgttgaag tataacaaag acataattgt tggagggact gatgaagtgt gttttgcgaa
     6961 ggcagtggtc gcaaccattg ggattctaga aatagccgga ttttcaaagg gacaatttca
     7021 accaatcttt gacggatgta aaattttcaa tgacttgaat ttggatgccg caatgggagc
     7081 tttgtactca gggaagaaat cagcatactt tgacggagca acaagcgacg agatcaatga
     7141 attctttgaa ctgagtgcag cgaagctact cagtaacgga catggagtat ggtctggttt
     7201 actcaaagct gagttgagac cgaaggcaaa ggtcgtggcg aacaaaacgc gaacattcac
     7261 atcagcacca attgatatac tcatgggtgc caaagctgtg gttgatgagt tcaacaaatt
     7321 cttctacaca aagcatttgc gcggaccatg gactgtcgga atcaataagt tcaacggagg
     7381 ttgggatttg ttggccaaaa atttaatggt gcacgagtgg ttcattgacg ctgatggttc
     7441 gcaattcgac agttcaatca ctccacttct catgaatgca attcttaaca tacggcaata
     7501 cttcatggca gaagatgatg aagctgaaca aatgctggca aatttgtata cgcagattat
     7561 aaacacatgc attttaattg aagatggaac gattgtgcag aagtttcgag gtaataacag
     7621 tggccaacca agcacagttg ttgacaacac gatgtgttta atcatagcaa tggagtattg
     7681 cagaatgcgc gtcgaaaagg atcatgaaca tagaatgagg atactgtacg tgtgcaacgg
     7741 agatgatttg cttattaatg ccgacacaaa ggacaaagac ttcatacagc agtattttgc
     7801 cgattacatg cgcgaactgg agctgaatta ctcatttgac gaggcttacc gtagtatcga
     7861 ggaggtggaa tacatgtcac atacatttat gaagcgaaat tcgatgtaca ttccaaagtt
     7921 gaaacgcgaa cgtattgtgg caattctgga gtggcagagg agcaaggaac cgaaggctat
     7981 tcagagcgct attattgcag catacgtgga agctttcggt tatgatgaat tcacggagat
     8041 gattgaagaa cttgcgcagg aagtttcagc ggtgtggccg gatttcaagt tgccctcacg
     8101 acaagaggtt gaggatttgt acttgactgg gacccgaacg gatttaggag aagagattaa
     8161 ggaatgtgga gagcaatact gcgtgtacga atcgagtgag gccgcaaccg acgctgtctt
     8221 ggcggcagca aatgcaggaa ctggtagtgc atcgagtagt ggaagcactc agtcaagtca
     8281 gagcgcaagt actgctagcg gatcagggag ttcaccatca ggatcaggtt ctggagcagc
     8341 gggtggatca ggttctggat cagcacaaac acaatctaat aacgtatctg tcatggctgg
     8401 cctcgacacg ggaggagcta agacagatca aggatcagga tcaaaaggga cgggtggttc
     8461 attcacatcg aatcccgtgc gaactggagg ccgagcaacg gatgtgcaag atcagacacc
     8521 aggtttagtg tttccagcac caaagatcac aacaaaggcc atatacatgc caaaaactgt
     8581 acgcgacaag ataaaacctg aaatgataaa taacatgatc aaataccaac cgcgtgctga
     8641 acttatcgac aacagatatg ccacaactga acaactcaac acctggataa aagaggcatc
     8701 tgaagggctt gacgtgacag aggatgtttt cataaacacc ttacttccag gatgggtcta
     8761 ccactgcata atcaacacaa cgagcccaga gaacagagca ctaggaactt ggcgtgttgt
     8821 gaacaatgca ggcaaggaca atgagcagca actcgagttt aagattgaac cgatgtacaa
     8881 agctgcgaag ccatcacttc gagccattat gcgccacttt ggtgagggag ctcgagtgat
     8941 gatcgaggag agtgttcgaa ttggaaaacc tatcatacca aggggcttcg acaaggccgg
     9001 tgtgctaagc atcaacaata ttgtggcagc gtgtgatttc atcatgcgcg gtgcagatga
     9061 cacaccaaat tttgtgcaag tgcagaacag cgttgcagta aacaggctac gcggaataca
     9121 gaacaagctg tttgcacagg cacgactgag tgcgggtact aatgaggaca actcacgtca
     9181 tgatgcagat gatgtgaggg agaacacgca cagtttcaat ggtgtaaacg ctcttgcgtg
     9241 agcacagtag aaactacaaa tccacgagta ccaggatttg agcgaaataa cgctgcgttt
     9301 cgttaaccct tcgttgttac taggtgtgta cttctccacg agaggcgtgc attcttggta
     9361 gctatgtgtg ggttagggcg acgctacaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     9421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     9481 aaaaaaaaaa aagcggccgc actagcggag tgtatactgg cttactatgt tggcactgat
     9541 gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc
     9601 agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg
     9661 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca
     9721 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg
     9781 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     9841 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc
     9901 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga
     9961 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
    10021 agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg
    10081 caaaagcacc actggcagca gccactggta attgatttag aggagttagt cttgaagtca
    10141 tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag
    10201 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg
    10261 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat
    10321 cttattaagg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
    10801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
    11101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11461 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatg
    11521 cacgaggccc tttcgtcttc aagaatttta taaaccgtgg agcgggcaat actgagctga
    11581 tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg tcagcacgac gttcctgtcc
    11641 acggtacgcc tgcggccaaa tttgattcct ttcagctttg cttcctgtcg gccctcattc
    11701 gtgcgctcta ggatcctccg gcgttcagcc tgtgccacag ccgacaggat ggtgaccacc
    11761 atttgcccca tatcaccgtc ggtactgatc ccgtcgtcaa taaaccgaac cgctacaccc
    11821 tgagcatcaa actcttttat cagttggatc atgtcggcgt gtcgcggcca agacggtcga
    11881 gcttcttcac cagaatgaca tcaccttcct ccaccttcat cctcagcaaa tccagccctt
    11941 cccgatctgt tgaactgccg gatgccttgt cggtaaagat gcggttagct tttacccctg
    12001 catctttgag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg
    12061 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta
    12121 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg
    12181 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg
    12241 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
    12301 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
    12361 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
    12421 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
    12481 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
    12541 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
    12601 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
    12661 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
    12721 aaccggcgca ggaacactgc cagcgcatca gcaatatttt cacctgaatc aggatattct
    12781 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
    12841 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
    12901 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
    12961 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
    13021 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
    13081 gaccgattta ggtgacacta tag
//
