LOCUS       40924_46628        5434 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWSK29, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Wang RF, Kushner SR.
  TITLE     Construction of versatile low-copy-number vectors for cloning, 
            sequencing and gene expression in Escherichia coli
  JOURNAL   Gene 100, 195-199 (1991)
  PUBMED    2055470
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Maples VF, Kushner SR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-1997) Genetics, University of Georgia, Life 
            Sciences Bldg., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 5434)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5434)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 100, 
            195-199 (1991)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-1997) Genetics, University of Georgia, Life Sciences Bldg., 
            Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(147..1004)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1005..1109)
                     /label=AmpR promoter
     rep_origin      complement(1135..1590)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1732..1748
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1758..1776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(1785..1892)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(1905..1923)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1944..1960)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1968..1984)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1992..2022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2037..2058)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_difference 2641
                     /label=pSC101
                     /note="pSC101"
     misc_difference 2830
                     /replace="c"
                     /label=pSC101
                     /note="pSC101"
     rep_origin      3337..3559
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3607..4554
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     misc_difference 4633
                     /replace="g"
                     /label=pSC101
                     /note="pSC101"
     misc_difference 4723
                     /replace="t"
                     /label=pSC101
                     /note="pSC101"
     misc_difference 4997..4999
                     /label=pSC101
                     /note="pSC101"
     misc_feature    5271..5431
                     /note="originates from pLG339; contains portion of tn903 
                     (kanamycin resistance transposon)"
ORIGIN
        1 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
       61 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      181 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      241 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      301 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      361 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      421 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      481 tcgtcgtttg gtatggcttc attcagctcc ggttcccagc gatcaaggcg agttacatga
      541 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      601 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      661 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      721 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      781 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      841 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      901 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      961 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     1141 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1201 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1261 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1321 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     1381 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     1441 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     1501 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     1561 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     1621 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     1681 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     1741 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
     1801 tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta
     1861 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     1921 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1981 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2041 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2101 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2281 aagaacatgt gagatctcta cgggtcggat ttgaagtcgt cttggtagga ggcagcctga
     2341 atggcgaatg ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     2401 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     2461 gggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     2521 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     2581 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     2641 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     2701 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     2761 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa
     2821 cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga
     2881 acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
     2941 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg
     3001 attcaccact ccgcagaccc gccataaaac gccctgagaa gcccgtgacg ggcttttctt
     3061 gtattatggg tagtttcctt gcatgaatcc ataaaaggcg cctgtagtgc catttacccc
     3121 cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga cagcgactca
     3181 ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg
     3241 ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac
     3301 atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg gctgggatct
     3361 attcttttta tcttttttta ttctttcttt attctataaa ttataaccac ttgaatataa
     3421 acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga atttacaagt
     3481 tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa aaggactagt
     3541 aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct agaccaattg
     3601 agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag tcgctatgac
     3661 ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa ccccacgatt
     3721 gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata cgctcagatg
     3781 atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag agagctgatg
     3841 acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt ccagtggaca
     3901 aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga gatattgcct
     3961 tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa gtcttttgaa
     4021 aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca aaagaaaact
     4081 cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat gcttgaaaat
     4141 aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag taaagattta
     4201 aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac tgatacgttg
     4261 attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga gaacaaccag
     4321 ataaaaatga atggtgacaa aataccaaca accattacat cagattccta cctacataac
     4381 ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac cagttttgag
     4441 gcaaaatttt tgagtgacat gcaaagtaag tatgatctca atggttcgtt ctcatggctc
     4501 acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag gatctgaggt
     4561 tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa gtagaacaac
     4621 tgttcaccgt tacatatcaa agggaaaact gtccatatgc acagatgaaa acggtgtaaa
     4681 aaagatagat acatcagagc ttttacgagt ttttggtgca ttcaaagctg ttcaccatga
     4741 acagatcgac aatgtaacag atgaacagca tgtaacacct aatagaacag gtgaaaccag
     4801 taaaacaaag caactagaac atgaaattga acacctgaga caacttgtta cagctcaaca
     4861 gtcacacata gacagcctga aacaggcgat gctgcttatc gaatcaaagc tgccgacaac
     4921 acgggagcca gtgacgcctc ccgtggggaa aaaatcatgg caattctgga agaaatagcg
     4981 ctttcagccg gcaaaccggc tgaagccgga tctgcgattc tgataacaaa ctagcaacac
     5041 cagaacagcc cgtttgcggg cagcaaaacc cgtacttttg gacgttccgg cggttttttg
     5101 tggcgagtgg tgttcgggcg gtgcgcgcaa gatccattat gttaaacggg cgagtttaca
     5161 tctcaaaacc gcccgcttaa caccatcaga aatcctcagc gcgattttaa gcaccaaccc
     5221 ccccccgtaa cacccaaatc catactgaaa gtggctttgt tgaataaatc agatttcggg
     5281 taagtctccc ccgtagcggg ttgtgttttc aggcaatacg cacgctttca ggcatacctg
     5341 ctttcgtcat tttgttcagc gctcgtacca gggccatagc ctccgcaacc tgaccatcgt
     5401 agtcacgcag cgtcagtgaa cccccgaaca gaga
//
