LOCUS       V015743                 5249 bp    DNA     circular SYN 09-JAN-2021
DEFINITION  Exported.
ACCESSION   V015743
VERSION     V015743
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Beitzel B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2020) Center for Genome Sciences, US Army Medical
            Research Institute of Infectious Disease, 1425 Porter St, Ft
            Detrick, MD 21702, USA
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNAstar / SeqMan Ngen v. 12/2015 Sequencing
            Technology      :: PacBio
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-NOV-2020) Center for Genome Sciences, US Army Medical Research
            Institute of Infectious Disease, 1425 Porter St, Ft Detrick, MD
            21702, USA"
FEATURES             Location/Qualifiers
     source          1..5249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(302..1114)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     enhancer        1344..1724
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1725..1928
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2064..2889
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             2919..3938
                     /gene="VP35"
                     /label="Polymerase cofactor VP35"
                     /note="Polymerase cofactor VP35 from Zaire ebolavirus
                     (strain Kikwit-95). Accession#: Q6V1Q9"
     polyA_signal    3966..4190
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4418..5006)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gggggggggg ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
       61 tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg
      121 taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
      181 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc
      241 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
      301 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
      361 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
      421 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
      481 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
      541 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
      601 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
      661 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
      721 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
      781 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
      841 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
      901 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
      961 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     1021 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     1081 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     1141 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     1201 gattttgaga cacaacgtgg ctttcccccc ccccccggca tgcctgcagg tcgacaatat
     1261 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     1321 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     1381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     1441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccggccc cctattgacg
     1621 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     1681 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1741 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1801 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1861 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1921 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     1981 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc
     2041 ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg cacacccctt
     2101 tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc gcttccttat
     2161 gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt attgaccact
     2221 cccctattgg tgacgatact ttccattact aatccataac atggctcttt gccacaacta
     2281 tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac tctgtatttt
     2341 tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg ccgtcccccg
     2401 tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg gtacgtgttc
     2461 cggacatggg ctcttctccg gtagcggcgg agcttccaca tccgagccct ggtcccatgc
     2521 ctccagcggc tcatggtcgc tcggcagctc cttgctccta acagtggagg ccagacttag
     2581 gcacagcaca atgcccacca ccaccagtgt gccgcacaag gccgtggcgg tagggtatgt
     2641 gtctgaaaat gagctcggag attgggctcg caccgctgac gcagatggaa gacttaaggc
     2701 agcggcagaa gaagatgcag gcagctgagt tgttgtattc tgataagagt cagaggtaac
     2761 tcccgttgcg gtgctgttaa cggtggaggg cagtgtagtc tgagcagtac tcgttgctgc
     2821 cgcgcgcgcc accagacata atagctgaca gactaacaga ctgttccttt ccatgggtct
     2881 tttctgcagt caccgtccaa gcttgcggcc gcgccaccat gaccaccaga acaaagggca
     2941 gaggccacac cgtggccacc acccagaacg acagaatgcc tggccctgag ctgagcggct
     3001 ggatcagcga gcagctgatg accggcagaa tccccgtgaa cgacatcttc tgcgacatcg
     3061 agaacaaccc cggcctgtgc tacgccagcc agatgcagca gaccaagccc aaccccaaga
     3121 tgcggaacag ccagacccag accgacccca tctgcaacca cagcttcgag gaagtggtgc
     3181 agaccctggc ctctctggcc actgtggtgc agcagcagac aatcgccagc gagagcctgg
     3241 aacagcggat caccagcctg gaaaacggcc tgaagcccgt gtacgatatg gccaagacca
     3301 tcagcagcct gaacagagtg tgcgccgaga tggtggccaa atacgacctg ctcgtgatga
     3361 ccaccggcag agccacagct acagccgccg ctacagaagc ctattgggcc gagcatggcc
     3421 agcctccacc tggccctagc ctgtacgagg aaagcgccat ccggggcaag atcgagagcc
     3481 gggatgagac agtgcctcag agcgtgcgcg aggccttcaa caacctggac agcaccacct
     3541 ccctgaccga ggaaaacttc ggcaagcccg acatcagcgc caaggacctg cggaacatta
     3601 tgtacgacca tctgcccggc ttcggcaccg ccttccatca gctggtgcaa gtgatctgca
     3661 agctgggcaa ggacagcaac tccctggaca tcatccacgc cgagttccag gccagcctgg
     3721 ccgaaggcga ttctcctcag tgtgccctga tccagatcac caagcgggtg cccatcttcc
     3781 aggatgccgc ccctcccgtg atccacatca gaagcagagg cgacatcccc agagcctgcc
     3841 agaaatccct gaggcccgtg ccccctagcc ccaagatcga tagaggatgg gtgtgcgtgt
     3901 tccagctgca agacggcaag accctgggcc tgaagatttg aagatctacg tatgatcagc
     3961 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4021 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4081 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4141 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4201 ggaaagaacc agctggggct cgacagctcg actctagaat tgcttcctcg ctcactgact
     4261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5221 tatttcgttc atccatagtt gcctgactc
//
