LOCUS       V015603                 5092 bp    DNA     circular SYN 09-JAN-2021
DEFINITION  Exported.
ACCESSION   V015603
VERSION     V015603
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Beitzel B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2020) Center for Genome Sciences, US Army Medical
            Research Institute of Infectious Disease, 1425 Porter St, Ft
            Detrick, MD 21702, USA
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNAstar / SeqMan Ngen v. 12/2015 Sequencing
            Technology      :: PacBio
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-NOV-2020) Center for Genome Sciences, US Army Medical Research
            Institute of Infectious Disease, 1425 Porter St, Ft Detrick, MD
            21702, USA"
FEATURES             Location/Qualifiers
     source          1..5092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(302..1114)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     enhancer        1344..1724
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1725..1928
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2064..2888
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             2918..3781
                     /gene="VP30"
                     /label="Transcriptional activator VP30"
                     /note="Transcriptional activator VP30 from Zaire ebolavirus
                     (strain Kikwit-95). Accession#: Q77DJ5"
     polyA_signal    3809..4033
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4261..4849)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gggggggggg ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
       61 tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg
      121 taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
      181 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc
      241 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
      301 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
      361 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
      421 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
      481 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
      541 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
      601 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
      661 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
      721 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
      781 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
      841 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
      901 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
      961 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     1021 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     1081 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     1141 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     1201 gattttgaga cacaacgtgg ctttcccccc ccccccggca tgcctgcagg tcgacaatat
     1261 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     1321 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     1381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     1441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccggccc cctattgacg
     1621 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     1681 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1741 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1801 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1861 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1921 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     1981 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc
     2041 ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg cacacccctt
     2101 tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc gctccttatg
     2161 ctataggtga tggtatagct tagcctatag gtgtgggtta ttgaccatta ttgaccactc
     2221 ccctattggt gacgatactt tccattacta atccataaca tggctctttg ccacaactat
     2281 ctctattggc tatatgccaa tactctgtcc ttcagagact gacacggact ctgtattttt
     2341 acaggatggg gtcccattta ttatttacaa attcacatat acaacaacgc cgtcccccgt
     2401 gcccgcagtt tttattaaac atagcgtggg atctccacgc gaatctcggg tacgtgttcc
     2461 ggacatgggc tcttctccgg tagcggcgga gcttccacat ccgagccctg gtcccatgcc
     2521 tccagcggct catggtcgct cggcagctcc ttgctcctaa cagtggaggc cagacttagg
     2581 cacagcacaa tgcccaccac caccagtgtg ccgcacaagg ccgtggcggt agggtatgtg
     2641 tctgaaaatg agctcggaga ttgggctcgc accgctgacg cagatggaag acttaaggca
     2701 gcggcagaag aagatgcagg cagctgagtt gttgtattct gataagagtc agaggtaact
     2761 cccgttgcgg tgctgttaac ggtggagggc agtgtagtct gagcagtact cgttgctgcc
     2821 gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc catgggtctt
     2881 ttctgcagtc accgtccaag cttgcggccg cgccaccatg gaagcctctt acgagagagg
     2941 cagacccaga gccgccagac agcacagcag agatggccac gatcaccacg tgcgggccag
     3001 aagcagctcc agagagaact accggggcga gtaccggcag agcagatcag cttctcaagt
     3061 gcgggtgccc accgtgttcc acaagaaacg ggtggaaccc ctgaccgtgc cccctgcccc
     3121 taaggatatc tgccctaccc tgaagaaagg cttcctgtgc gacagcagct tctgcaagaa
     3181 ggaccaccag ctggaaagcc tgaccgacag agaactgctg ctgctgatcg ccagaaagac
     3241 ctgcggcagc gtggaacagc agctgaacat caccgccccc aaggacagca gactggccaa
     3301 ccctaccgcc gacgacttcc agcaggaaga gggccccaag atcaccctgc tgaccctgat
     3361 caagaccgcc gagcactggg ccagacagga catcaggacc atcgaggaca gcaagctgcg
     3421 ggctctgctg acactgtgcg ccgtgatgac ccggaagttc agcaagagcc agctgagcct
     3481 gctgtgcgag acacacctga gaagagaggg cctgggccag gatcaggccg agcctgtgct
     3541 ggaagtgtac cagagactgc acagcgacaa gggcggcagc tttgaagccg ctctgtggca
     3601 gcagtgggac agacagagcc tgattatgtt tatcaccgcc tttctgaata tcgccctgca
     3661 gctgccctgc gagtctagcg ctgtggtggt gtctggcctg agaaccctgg tgccccagag
     3721 cgacaatgag gaagccagca ccaaccccgg cacctgttct tggagcgacg agggcacacc
     3781 ctgaagatct acgtatgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3841 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3901 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3961 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4021 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacagc tcgactctag
     4081 aattgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4141 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4201 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4261 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4321 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4381 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4441 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4501 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4561 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4621 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4681 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4741 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4801 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4861 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4921 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4981 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5041 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tc
//
