LOCUS       V015958                 6446 bp    DNA     circular SYN 09-JAN-2021
DEFINITION  Exported.
ACCESSION   V015958
VERSION     V015958
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Beitzel B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2020) Center for Genome Sciences, US Army Medical
            Research Institute of Infectious Disease, 1425 Porter St, Ft
            Detrick, MD 21702, USA
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNAstar / SeqMan Ngen v. 12/2015 Sequencing
            Technology      :: PacBio
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-NOV-2020) Center for Genome Sciences, US Army Medical Research
            Institute of Infectious Disease, 1425 Porter St, Ft Detrick, MD
            21702, USA"
FEATURES             Location/Qualifiers
     source          1..6446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(302..1114)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     enhancer        1344..1724
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1725..1928
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2064..2889
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             2919..5135
                     /gene="NP"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Zaire ebolavirus (strain
                     Kikwit-95). Accession#: O72142"
     polyA_signal    5163..5387
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(5615..6203)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gggggggggg ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
       61 tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg
      121 taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
      181 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc
      241 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
      301 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
      361 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
      421 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
      481 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
      541 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
      601 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
      661 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
      721 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
      781 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
      841 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
      901 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
      961 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     1021 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     1081 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     1141 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     1201 gattttgaga cacaacgtgg ctttcccccc ccccccggca tgcctgcagg tcgacaatat
     1261 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     1321 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     1381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     1441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccggccc cctattgacg
     1621 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     1681 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1741 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1801 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1861 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1921 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     1981 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc
     2041 ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg cacacccctt
     2101 tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc gcttccttat
     2161 gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt attgaccact
     2221 cccctattgg tgacgatact ttccattact aatccataac atggctcttt gccacaacta
     2281 tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac tctgtatttt
     2341 tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg ccgtcccccg
     2401 tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg gtacgtgttc
     2461 cggacatggg ctcttctccg gtagcggcgg agcttccaca tccgagccct ggtcccatgc
     2521 ctccagcggc tcatggtcgc tcggcagctc cttgctccta acagtggagg ccagacttag
     2581 gcacagcaca atgcccacca ccaccagtgt gccgcacaag gccgtggcgg tagggtatgt
     2641 gtctgaaaat gagctcggag attgggctcg caccgctgac gcagatggaa gacttaaggc
     2701 agcggcagaa gaagatgcag gcagctgagt tgttgtattc tgataagagt cagaggtaac
     2761 tcccgttgcg gtgctgttaa cggtggaggg cagtgtagtc tgagcagtac tcgttgctgc
     2821 cgcgcgcgcc accagacata atagctgaca gactaacaga ctgttccttt ccatgggtct
     2881 tttctgcagt caccgtccaa gcttgcggcc gcgccaccat ggatagcaga ccccagaaag
     2941 tgtggatgac ccccagcctg accgagagcg acatggacta ccacaagatc ctgacagccg
     3001 gcctgagcgt gcagcaggga attgtgcggc agcgcgtgat ccccgtgtac caagtgaaca
     3061 acctggaaga gatttgccag ctgatcatcc aggccttcga ggccggcgtg gacttccagg
     3121 aaagcgccga tagcttcctg ctgatgctgt gcctgcacca cgcctaccag ggcgactaca
     3181 agctgttcct ggaatctggc gccgtgaagt acctggaagg acacggcttc agattcgaag
     3241 tgaagaaatg cgacggcgtg aagcggctgg aagaactgct gcctgccgtg tccagcggcc
     3301 ggaacatcaa gagaacactg gccgccatgc ccgaggaaga gacaaccgaa gccaacgccg
     3361 gccagttcct gagcttcgcc agcctgttcc tgcccaagct ggtcgtggga gagaaggcct
     3421 gcctggaaaa ggtgcagcgg cagatccagg tgcacgccga gcagggcctg atccagtatc
     3481 ctacagcctg gcagagcgtg ggccacatga tggtcatctt ccggctgatg aggaccaact
     3541 tcctgatcaa gtttctgctg atccaccagg gcatgcacat ggtggccgga cacgacgcca
     3601 acgacgccgt gatcagcaat tctgtggccc aggccagatt cagcggcctg ctgatcgtga
     3661 aaaccgtgct ggaccacatc ctgcagaaaa ccgagagagg cgtgcggctg cacccactgg
     3721 ccagaaccgc caaagtgaaa aacgaagtga acagcttcaa ggccgccctg agcagcctgg
     3781 ccaagcacgg cgaatatgcc cccttcgcca gactgctgaa cctgagcggc gtgaacaatc
     3841 tggaacacgg cctgtttccc cagctgagcg ccattgctct gggagtggcc acagcccacg
     3901 gaagcacact ggctggcgtg aacgtgggcg agcagtacca gcagctgaga gaggccgcca
     3961 cagaggccga gaaacagctg cagcagtacg ccgagagcag agagctggat cacctgggcc
     4021 tggacgacca ggaaaagaaa atcctgatga acttccacca gaagaagaac gagatcagct
     4081 tccagcagac caacgccatg gtcaccctgc ggaaagagcg gctggccaaa ctgaccgagg
     4141 ccatcacagc cgcctccctg cctaagacaa gcggccacta cgacgacgat gacgacatcc
     4201 ccttccctgg ccccatcaat gacgacgaca accctggcca ccaggacgac gaccctaccg
     4261 acagccagga caccaccatc cccgacgtgg tggtggaccc tgatgatggc ggctacggcg
     4321 agtaccagag ctacagcgag aacggcatga gcgcccctga cgacctggtg ctgttcgacc
     4381 tggacgagga cgatgaggat accaagcccg tgcccaacag aagcaccaag ggcggccagc
     4441 agaagaacag ccagaagggc cagcacaccg agggcagaca gacccagagc acccccaccc
     4501 agaatgtgac cggccccaga agaaccatcc accacgcaag cgcccctctg accgacaacg
     4561 acagaagaaa cgagcccagc ggcagcacca gccccaggat gctgaccccc attaacgagg
     4621 aagccgaccc cctggacgat gccgacgacg agacatctag cctgcccccc ctggaatccg
     4681 atgacgagga acaggacagg gacggcacca gcaacagaac ccctacagtg gcccctcccg
     4741 cccctgtgta cagagatcac tccgagaaga aagagctgcc ccaggacgag cagcaggacc
     4801 aggatcacat ccaggaagcc cggaaccagg acagcgacaa cacccagccc gagcacagct
     4861 tcgaggaaat gtaccggcac atcctgagat cccagggccc cttcgatgcc gtgctgtact
     4921 atcacatgat gaaggacgag cccgtggtgt tcagcacctc cgacggcaaa gagtacacct
     4981 accccgacag cctggaagaa gagtaccccc cttggctgac agagaaagaa gccatgaacg
     5041 acgagaaccg cttcgtgacc ctggatggcc agcagtttta ctggcccgtg atgaaccacc
     5101 ggaacaagtt catggccatt ctgcagcacc accagtgaag atctacgtat gatcagcctc
     5161 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5221 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5281 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5341 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5401 aagaaccagc tggggctcga cagctcgact ctagaattgc ttcctcgctc actgactcgc
     5461 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5521 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6181 cgcgcataaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6241 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6301 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6361 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6421 ttcgttcatc catagttgcc tgactc
//
