LOCUS       40924_46588       12689 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression shuttle vector pWLG30, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Expression vectors for Methanococcus maripaludis: overexpression of 
            acetohydroxyacid synthase and beta-galactosidase
  JOURNAL   Genetics 152 (4), 1439-1447 (1999)
  PUBMED    10430574
REFERENCE   2  (bases 1 to 12689)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-1999) Microbiology, University of Georgia, 
            Biological Sciences Building, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 12689)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12689)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1999"; volume: "152"; issue: "4"; pages: "1439-1447"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-1999) Microbiology, University of Georgia, Biological 
            Sciences Building, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      61..69
                     /note="PhmvA BoxA; Methanococcus voltae histone promoter
                     Box A"
                     /regulatory_class="promoter"
     regulatory      92..95
                     /note="PhmvA BoxB; Methanococcus voltae histone promoter
                     Box B"
                     /regulatory_class="promoter"
     regulatory      153..158
                     /regulatory_class="ribosome_binding_site"
     misc_feature    160..205
                     /label=polylinker
                     /note="polylinker"
     regulatory      464..471
                     /note="Pmcr Box A; promoter for Methanococcus voltae methyl
                     coenzyme M reductase"
                     /regulatory_class="promoter"
     CDS             988..1587
                     /codon_start=1
                     /gene="pac"
                     /product="puromycin transacetylase"
                     /label=pac
                     /note="from Streptomyces alboniger"
                     /protein_id="AAF01148.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTRAARTLPHARRARHRQGVGRATRRRGGGLDHAGERRSGGGVRRDRNAHGRVERFPAG
                     RAATDGRPPGAAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRSGGGRARRGARLPGDL
                     RAPQPPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     gene            988..1587
                     /gene="pac"
                     /label=pac
     regulatory      1887..1912
                     /note="terminator for Methanococcus voltae methyl coenzyme
                     m reductase"
                     /regulatory_class="terminator"
     promoter        2266..2370
                     /label=AmpR promoter
     CDS             2371..3228
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3402..3990
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4278..4299
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4314..4344
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4352..4368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4376..4392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6816..7188
                     /label=ORFLESS2
                     /note="ORFLESS2"
     misc_feature    7948..8733
                     /label=ORFLESS1
                     /note="ORFLESS1"
ORIGIN
        1 ttcagctgat cgatcaaaat ataacataaa taacataggt ttaaataatt taaaggcata
       61 ttttatataa acaattgtaa aatattggct tatgaaattt gttaaaattt agctaataag
      121 ctattgatat tataatttta tagataacta ataggtgaaa tgcatgttta gcgctgaaga
      181 tctcatgata tctagatcct ctagaggatg attaatttta agagagtctt aaaaaatctt
      241 cggaaaatag tagtaggtaa taaaaaacgc cctattcgta atatactatt caaaactaaa
      301 tattcattga aatatttggg taataattaa tattaatcta atatttagat attgagatat
      361 aataataaca tttttttaaa aaatcatcta ttttcatctt aattttatat attaaagccc
      421 attttttgag tattcaaatt caaattactg tgttattaac atcttatata taaacttttc
      481 tatttaatgt taatgaaaaa gtgaatatat atacatagag taatgttatg atgtatatat
      541 caaaaaaata ggagtgattc ctatggtaaa gtatgaagat aagatatgtc tatttgatgc
      601 gaaaggaaac caagtagcag aagatgtacc attggaagct atcagcccat taaataaccc
      661 aactataatg gggatggtta aaaatattaa aagaaccgtt gctgttgacg gatccccagc
      721 ttgcatgcct gcaggtcgac atcgcggccg aaccggttcg tgagcccctg ggacatcgcc
      781 gccctccaga tcctcgtgga ggaggcgggc ggcgtctgca ccgacctgct cggcggctcg
      841 ccccagcgcg ggacgggtgc cctgtccgcc aacccggagc tgcaccggct cgccgtggag
      901 gccctcgcgc ccccgcggcc gccacgaccg gtgccgccac catcccctga cccacgcccc
      961 tgaccccaca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc
     1021 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     1081 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcacccg agctgcaaga
     1141 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgc cacacggcgc
     1201 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     1261 cggaacgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     1321 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     1381 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     1441 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tcccttctac
     1501 gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg
     1561 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgagccga
     1621 aaggagcgca cgaccccatg gctccgaccg aagccgaccc gggcggcccc gccgaccccg
     1681 cacccgcccc cgaggcccac cgcgggggac acaccgaaca cgccgaccct gctgaacacg
     1741 cggcgcagtt cggtgcccag gagcggatcg ggaattaatt cgaagctgct ggtgaaagag
     1801 accctatctt acctgctaaa atctaagtta attactaatt tattattaat ttattattag
     1861 attgggcaaa atagtaaaag aaaactaaag gaaacctaat atggtttcct ttttttatat
     1921 atttttaatt cactgggggg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1981 gatccttggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     2041 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2101 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2161 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2221 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2281 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2341 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2401 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2461 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2521 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2581 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2641 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2701 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2761 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2821 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2881 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2941 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3001 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3061 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3121 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3181 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3241 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3301 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3541 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4201 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4261 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     4321 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     4381 aacagctatg accatgatta cgaattcttt aaatgatagt gaaaaaatca actggaacaa
     4441 aataaaagtt taagagggag attatgaagc aacattttac acttataaaa ccaatggatg
     4501 caaaagctgt gagatactat tttcataaat tggagaattt aaacgatgaa atagatttag
     4561 acattttgat taaagctata actacaaaca aaccatttaa aagaccttta acccttacag
     4621 aagaagaaga gggaattatt aatagatatg ggagggctac aaacacatta actaattata
     4681 tgatattaaa agaatcaaaa acccacgtat aggtgtttaa tatggaaaat aatatatcta
     4741 atataaggaa aaccttttta ttatgtcgtg aggtctccgc taaagatatt agatactatt
     4801 tgtataaatt ggagaattta ggcgagataa accccgaagt attagcaaaa gcggaaaact
     4861 gcgaaaaacc aaaaaaatta ttattgacat tgggtaagca tgatagaaga attgttgaaa
     4921 aatacggaaa aatgacaaat acacttttaa attaccaatt aataacagaa tatcccgaag
     4981 gggtagaata tgaatgaaat aattgataat tacagaaaag aaagggaatt aagggggata
     5041 agcccgaaaa cattgcagag cgaaatattt aatttaaaat atttcttatc tttttgtaaa
     5101 gataataatt tatcgctttt ttcattgaat acctgtgatt ttgagttatt ttttaataat
     5161 ctaaaatata atcgtaattg tagtaaatct acccaaaaaa attattttat aatgctaaaa
     5221 catttattta gatacttgaa aatagataat ttcaaagatt atgaagtaaa agcaataaac
     5281 aacaagatat attctattaa aaaagtagaa cactatgata tattagattt agagacttat
     5341 aaaaacatat taagggaaat attaaaaagg gaaagtagaa caggcattag ggacgcatta
     5401 attataaaaa tattgtggga aacaggctgt acacgttctg aagttgtaaa attacaaaat
     5461 aaagattttg cattagaaac aggtatttta agaataaaaa atagaaaagg atatctaaat
     5521 aataaagata gaaacgtgat tgtatcagaa aacacagtta aattaattaa taaatacgtg
     5581 tttgaaaacg tgaagaaagg ccccaatgat tatatttttc agaatgaaaa gaatatgaat
     5641 ggaaatccag ttaaacccga ttttataacc catgtattta aaaccgctgt ggatacgttg
     5701 aaagataata aaataatccc taaagataaa aatatagtgc ttcagtcctt aagatatggg
     5761 agagcgcagg aattatatga taaaggagta gccattgaga atataaaaac attattgggt
     5821 agtaaataca tagaaactat tttaaaaaga gatattgaag aaaataaaaa taaagtgctt
     5881 gaagatattc aaaaactact ttaaaagtgt aaattgcact taaaagaaaa gatattatct
     5941 tttctttcgt tgtcctttgt tcaaattgcc ttaattttgc tgtgctttat caagcttcac
     6001 gctggttcat cttcgtaaat ccccagtgtt aatttaacag actttaaaac caccttcaaa
     6061 gattaagaag gcatttaatt atattaacca attttatata tatatttaat tgaaaaatat
     6121 aaaatatatt taaaatcttt ctattttcaa aaatactatt tataaaaacg ttattagata
     6181 aatagataaa tatctatttc taaaatagta attagatata aaatacttca tattttgggg
     6241 gaattataaa aatggtgcgt atttatggaa aatggaaacg attttgagat aatagaagat
     6301 tgtttgaagt tatgttttaa gatagaagct tcaaatcaat ttatgaagca gaaaaacgcc
     6361 gttaaaatat taatgaatat agaaaacaga ttccagacca tgaaagataa tgaaaaaatc
     6421 gtttttaaag aattggaatg caatttaaaa gcagtttata aaatagagtc ctttgaaacc
     6481 atagacgcac aaaaaggatt taaaaaagtc tgtgaacatc tggaagaaat taaaaagaat
     6541 ttatattgtt tattaaataa atacaaaaat tcaaaatgat atatataaag tatcaaaata
     6601 tcataaagat atatataaat accctcaatt acatttatat aatcccaaaa taagttataa
     6661 attgatttat ttttttacct tgaattgttg cagaatactg gaacctgcga cttactccag
     6721 tattcattca agtttacccc gaataagaaa gggtgttgag atttcaagaa cataatacac
     6781 gatatcgtgt agttcgcccc aggaataaaa tacacgatat cgtgtagact ccttcagtat
     6841 ttttaaaaaa aatttcaaaa ctatttattt tgttaaaaga gtttaattat aattttcata
     6901 gctaaaaaca attaaaacac attgaaaaaa ataattaaac ccttttaaag aaatcaaaac
     6961 attaaaaatc attttcattt tatagtttaa tttaccaacc agtacctgaa ccctgcattt
     7021 ttgaaggttt gaagtatagg caaaaataaa gaagaaatat gccccccgcg agaaataaaa
     7081 gaaccaataa ataaaccctt tttctaaaca gttgagtaaa tagaaataaa aaagaaatat
     7141 aaatgggaaa aattaaaaaa tacatatgaa atatagttta aaatatgttt ggattatttt
     7201 tataaagaga ataataaaaa taaaaaagga gtggggagta tattaagtaa gattaaaaaa
     7261 acaattgaaa ataaagaagg aaaagagaaa aaaacaataa tagaagggga taaaaccgca
     7321 gaactaacca gaataaataa taagctagat aatattgata aaatagtgaa tgaacattta
     7381 aatgactttg aattattagg aaaggcaatt gatgaatact tattaagtat aaaaaaagaa
     7441 ttaaaggaca atataaacgc agattctaaa aagaagatag aacagattaa taaaatacaa
     7501 aatacattaa aagaactgga aaatagattt aatgattttg aaaaagcagt acaagaagta
     7561 aaaacgataa gggaacggga taaaaagaac tggaaaatat taaatgaatg gcttcataat
     7621 gtagaagata gattgaagac gttagaacag gaaaagaaga acaataaaag aagatataat
     7681 gaaataaata ataagttcgc aggtattgaa aaatacatta atacagaaag gtataaaaaa
     7741 acaattaaga aagaaacaaa ccgcgagaac gtattatatt tattaaagaa cggattaaac
     7801 caacctacgg aaataaagaa agagtttaaa ggaggaacaa aagcattata tgaaacatta
     7861 aaaaggcttg aaaaatcggg agagattaca cgcaggaaag aaggaaagaa ggtttattat
     7921 gagataaaga aaggatataa tttataagga ctgaataaaa ccatattttt ttatacatat
     7981 ttatgaaaaa gaaggcattt aacgaccttt cagaagaaaa cagagaatga aaacagattt
     8041 taatttgtat ctggtaccca ttatttagtt tttttttatc accacaacgt ataaaccccc
     8101 tttcagaaga aaacagagaa tgaaaacaga ttttaattta tatatcgtat ctataattta
     8161 gttttttttt attgcagttt tagaagtgcg tttgtcctgg agcgaaatac acgatatcgt
     8221 gtagtctcct tcagatgaaa ataatatttt taattttcag aaatattact ggaaaaatac
     8281 ctgaaaaata tttgaaaaaa aaacatacac ccagcgggga aaaatcgaac attttgaaaa
     8341 agtctattta tagggtttaa acagggccaa gacctgcggg atatacccca aaaatgttcg
     8401 ataaaaaaat cgaacatttt tcgaacattt tttataatgt tcgatgtgtg cgagtatgtt
     8461 cgataaaaaa atcgaacatt ttgaaagaca atttgaagag atataaaaaa aataatctat
     8521 tataatatag atatataata tgttcgatat gttcgatatt tttttatata ttatattata
     8581 tttaaccttg tattgattat tgaataatat ttattattac tttttttgat ttcttgtaaa
     8641 aaatcgaaca tattttacat attgtaaaaa ataaaaaaga tttaaaaaaa gtgtccgaaa
     8701 aagttaaaca aacattatta ttaaaatgtt agtttaatat gtatcttgat atgctcttct
     8761 ttctttcatg cttaaacttg tataactgta tgcttttttc atttccttta ttttgtcctt
     8821 tcctttcttt gttatctggt aatatccttc cttctttcct gcatacttca aaaatccaag
     8881 gttttttaag ttaattaatg cccttttact ggtatcataa ctacaccacg ttaatatatt
     8941 cgctattggg gcgttaatta ctatcgggtg gttttctaaa gtgggtaaga ataataacaa
     9001 cattatagct tcagattcgt ttatatattc cagttccatt ttaccacctt ttaaattatt
     9061 ctgaatttac ctgtttttgg ttcgtctatt tccccattcc attttaaatt agttaatatt
     9121 tttcttgttt cgtctggttc gtggtctttt aaatgttcca ttaataaatc aaagcttatt
     9181 aatcctttat cgtttatatc atacagtatt tttaaagtat ctaatatttg gtcgcgtttc
     9241 gtgtattttg gggcgggttt ttctatgctt tcgacaatat cgcttaattg gtcgagattt
     9301 gttattatat acatatcgtt ttcaaatgtc gctattttca aagtttggag tatttttaaa
     9361 gtatctttta ctttcgtttc ttcgtggtta ttcaatagtt cttctgtgtc tatattcccc
     9421 gcgttttgaa tcgcaattgt tttaatgtcg cttaatactt cggggtgttc ttttaaaata
     9481 ttgtggtcca atataatatc aattttcaaa ccgtctttta ctgcgttagc ttccgcacct
     9541 attcttgttt ttcctatttc cgcgttatat cctttcttct gtattttttc taagaatgtt
     9601 tttagcttca ttttattagg gtaatttata cccttttctt tgagtttagg gagtaagtta
     9661 tttaatccag attgatttaa aaagattctc ttagtatttc cagtatgaaa caccgtatat
     9721 ttgtttaaat taaataattg tactgaatat ttatttaatg tttcaggcat tattgcctta
     9781 tcggggcttt tatcgggcat tgttgctatt ttcataaagt cgttataaat tatttcttct
     9841 attatctcca ttatggtttc atcgtttatt atgtctgttt caaactcttt ttcattgtct
     9901 tttaaccttg aaatgatgtt ctctactctt tcataactca aaacattaat attatttgaa
     9961 tcatagaatt cttttaatag cattaatgaa aatctaataa atttaaattg tgcttccctt
    10021 tccaatgtgt tttcatattt tgttttaact tctgaaggtg ttaaactcat tacgtaatca
    10081 ataaatattt ttttaagttc taatgtatta ttttttaaat attccaaact attttctatt
    10141 atttcccgtg gtatattgtc tgttttctca ttaatccgca gggtaatgca ccgccttaaa
    10201 aatgctttga tatcgtcttc attttcaaag ttaatagaat atgggtgatt acaggttatt
    10261 atatgcccta ttttaaacag aatcgtttca ttttcccgcc cgtaggattg tttaaactcg
    10321 ggggctgttg caccgtcttt tatcatgtcg cttattgttc ttctgaatct tgaaggttca
    10381 tcaaacaggt tatacccgca accgtacccc gcacacgttc taaataattg tggctctgtg
    10441 ggttctttcg ttttaacttc tgtaaaatta aaataacgtg cgagtgtcgt ttttccgtgt
    10501 tttgcttcac catataatat taattggggt gtaacttcat atttcaagat atttactaat
    10561 aaggttccca taaagtaagc ttcagttaaa atattatggt ctttatcaaa ttttgacaat
    10621 tccattaata ttttttcttt attttctact tttaactctt ttaaagtctt ttgaatgtct
    10681 gcgggtgttg gtaaagacct tgatttaaat atgtttggtg cgggttctac atttggaaat
    10741 attattttat tttcatgttc tgttaatact aataaacttg atttaatctc tattttctca
    10801 acagcgtttt taataatgtc cttaacaata tctattatct caaaatcctt tttaatgtat
    10861 tttttaaccg catcagtgga tatatattct aaaatatcct tcactttatc caactgtata
    10921 tctttagata taattttatt gcctattacg gcggttactt ctttaaggga gtaatatttt
    10981 ttgtttaaat atatatcgtg gattttagag atatttttaa atgtgaacaa tatcatctcg
    11041 ttctttgtag acatcaaata ttcttcctgt gtggtttgtg agatataatc aatgatatta
    11101 tgtgtttcac acaattctaa gataaattta tttcctgttt tccgtaatat catttttaac
    11161 gcttttattt cccttatctt gtgggtattg ctcgacctat ctatataatc taatttttca
    11221 tctaaatttt tgtatctggt tcttagttct tcggttttta cattatatct tgattcttta
    11281 tgaagtgttt tattaaatac ttcataatcc ccttttattc catttattaa aattattttt
    11341 tcgtttattt cctttttaat gtcctcagat tttttcttta tctcggcatc attagaattt
    11401 atgtcaaatt ctggaaaacc ttttttataa ccttcaaaaa tacctttttg aattgcagtt
    11461 gttaaaattg aaaatatatt ctcgtaataa tcaatgaatt cttttaaatc ctgcttgtaa
    11521 actttgcaat attccgcggg gtcaaaaata atagtttttt cgtaacattg ggatgatata
    11581 aattctggga tatccttttc accttttaaa aataactcat ttttcggtgt ggtggatata
    11641 ctggtatcat gttgtttaac cacggtttag cctcttgtat tgttgcatat atcattgtta
    11701 gacctgcgac agtctaaaaa atcaattaat tttcttttaa taatggtata aaaatattgt
    11761 gggtttaaac tcatttttta taacccaaac aagtttttta aactactctc ttcttcttca
    11821 gggagattat gaattatttc tatttttgct gtgtaaatta tgtaatttag aagtgtattt
    11881 gttgttttac catatttttt aataatttcc tcctcttctt tcgttaaagt gattgtttta
    11941 ttatatttcg tggggtattt cgaaagctcc cctaatattt gcgggtctaa tttttttata
    12001 tctttaattt tacttaagta aaatcttaaa gacctaacat tgagattttt ataaattctg
    12061 aaattatgtt gtatcttatc accttttttg aaatttcgaa atagtatata tataacttcg
    12121 aaaaatatat tttttgcaaa caatatatat agattttgca aaaaaaaata aagtgaaggt
    12181 ggaatggtgt ataaaatatg tcctaagtgt tattcgattg taagagtttt cgaccgtgat
    12241 tttatcaacg gggttataag accggttaaa gttccagagt gctgcgggtt tttccttact
    12301 ccatatattt ataaatccgc ggaatgttca aacatccaga aattaaaaac aactgcggga
    12361 gcactcgcaa aaatgatgaa actaaatgaa gaacagaaag cacagttttt taaaaacatt
    12421 attgaattta aaagaatgta caacgcacag aaagactaca aaatattaga attagcctta
    12481 aactcattag atattaaaac cctgtattca ttacccgaat taatagaatc ccctttcaaa
    12541 tcaagaaagg tacttccaaa agaaaccaaa cccgcgcaaa aggaacaaaa acccgaagta
    12601 aaaaaaccca taaagaaaga agagcctaaa ttaattaaaa aagactctcc agaatacata
    12661 aaatacagat taggcatgat aaacgggaa
//
