LOCUS       40924_46583        4404 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative expression vector pWLG14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Expression vectors for Methanococcus maripaludis: overexpression of 
            acetohydroxyacid synthase and beta-galactosidase
  JOURNAL   Genetics 152 (4), 1439-1447 (1999)
  PUBMED    10430574
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-1999) Microbiology, University of Georgia, 
            Biological Sciences Building, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 4404)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1999"; volume: "152"; issue: "4"; pages: "1439-1447"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-1999) Microbiology, University of Georgia, Biological 
            Sciences Building, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      61..69
                     /note="PhmvA BoxA; Methanococcus voltae histone promoter
                     Box A"
                     /regulatory_class="promoter"
     regulatory      92..95
                     /note="PhmvA BoxB; Methanococcus voltae histone promoter
                     Box B"
                     /regulatory_class="promoter"
     regulatory      153..158
                     /regulatory_class="ribosome_binding_site"
     misc_feature    160..205
                     /label=polylinker
                     /note="polylinker"
     regulatory      464..471
                     /note="Pmcr Box A; promoter for Methanococcus voltae methyl
                     coenzyme M reductase"
                     /regulatory_class="promoter"
     CDS             988..1587
                     /codon_start=1
                     /gene="pac"
                     /product="puromycin transacetylase"
                     /label=pac
                     /note="from Streptomyces alboniger"
                     /protein_id="AAF01146.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTRAARTLPHARRARHRQGVGRATRRRGGGLDHAGERRSGGGVRRDRNAHGRVERFPAG
                     RAATDGRPPGAAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRSGGGRARRGARLPGDL
                     RAPQPPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     gene            988..1587
                     /gene="pac"
                     /label=pac
     regulatory      1887..1912
                     /note="terminator for Methanococcus voltae methyl coenzyme
                     m reductase"
                     /regulatory_class="terminator"
     promoter        2266..2370
                     /label=AmpR promoter
     CDS             2371..3228
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3402..3990
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4278..4299
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4314..4344
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4352..4368
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4376..4392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcagctgat cgatcaaaat ataacataaa taacataggt ttaaataatt taaaggcata
       61 ttttatataa acaattgtaa aatattggct tatgaaattt gttaaaattt agctaataag
      121 ctattgatat tataatttta tagataacta ataggtgaaa tgcatgttta gcgctgaaga
      181 tctcatgata tctagatcct ctagaggatg attaatttta agagagtctt aaaaaatctt
      241 cggaaaatag tagtaggtaa taaaaaacgc cctattcgta atatactatt caaaactaaa
      301 tattcattga aatatttggg taataattaa tattaatcta atatttagat attgagatat
      361 aataataaca tttttttaaa aaatcatcta ttttcatctt aattttatat attaaagccc
      421 attttttgag tattcaaatt caaattactg tgttattaac atcttatata taaacttttc
      481 tatttaatgt taatgaaaaa gtgaatatat atacatagag taatgttatg atgtatatat
      541 caaaaaaata ggagtgattc ctatggtaaa gtatgaagat aagatatgtc tatttgatgc
      601 gaaaggaaac caagtagcag aagatgtacc attggaagct atcagcccat taaataaccc
      661 aactataatg gggatggtta aaaatattaa aagaaccgtt gctgttgacg gatccccagc
      721 ttgcatgcct gcaggtcgac atcgcggccg aaccggttcg tgagcccctg ggacatcgcc
      781 gccctccaga tcctcgtgga ggaggcgggc ggcgtctgca ccgacctgct cggcggctcg
      841 ccccagcgcg ggacgggtgc cctgtccgcc aacccggagc tgcaccggct cgccgtggag
      901 gccctcgcgc ccccgcggcc gccacgaccg gtgccgccac catcccctga cccacgcccc
      961 tgaccccaca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc
     1021 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     1081 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcacccg agctgcaaga
     1141 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgc cacacggcgc
     1201 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     1261 cggaacgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     1321 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     1381 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     1441 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tcccttctac
     1501 gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg
     1561 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgagccga
     1621 aaggagcgca cgaccccatg gctccgaccg aagccgaccc gggcggcccc gccgaccccg
     1681 cacccgcccc cgaggcccac cgcgggggac acaccgaaca cgccgaccct gctgaacacg
     1741 cggcgcagtt cggtgcccag gagcggatcg ggaattaatt cgaagctgct ggtgaaagag
     1801 accctatctt acctgctaaa atctaagtta attactaatt tattattaat ttattattag
     1861 attgggcaaa atagtaaaag aaaactaaag gaaacctaat atggtttcct ttttttatat
     1921 atttttaatt cactgggggg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1981 gatccttggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     2041 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2101 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2161 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2221 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2281 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2341 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2401 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2461 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2521 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2581 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2641 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2701 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2761 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2821 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2881 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2941 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3001 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3061 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3121 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3181 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3241 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3301 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3541 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4201 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4261 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     4321 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     4381 aacagctatg accatgatta cgaa
//
