LOCUS       40924_46578        4400 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative expression vector pWLG13, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Expression vectors for Methanococcus maripaludis: overexpression of 
            acetohydroxyacid synthase and beta-galactosidase
  JOURNAL   Genetics 152 (4), 1439-1447 (1999)
  PUBMED    10430574
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-1999) Microbiology, University of Georgia, 
            Biological Sciences Building, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 4400)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1999"; volume: "152"; issue: "4"; pages: "1439-1447"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-1999) Microbiology, University of Georgia, Biological 
            Sciences Building, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      50..58
                     /note="PhmvA BoxA; Methanococcus voltae histone promoter
                     Box A"
                     /regulatory_class="promoter"
     regulatory      81..84
                     /note="PhmvA BoxB; Methanococcus voltae histone promoter
                     Box B"
                     /regulatory_class="promoter"
     regulatory      142..147
                     /regulatory_class="ribosome_binding_site"
     misc_feature    150..201
                     /label=polylinker
                     /note="polylinker"
     regulatory      460..467
                     /note="Pmcr Box A; promoter for Methanococcus voltae methyl
                     coenzyme M reductase"
                     /regulatory_class="promoter"
     CDS             984..1583
                     /codon_start=1
                     /gene="pac"
                     /product="puromycin transacetylase"
                     /label=pac
                     /note="from Streptomyces alboniger"
                     /protein_id="AAF01144.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTRAARTLPHARRARHRQGVGRATRRRGGGLDHAGERRSGGGVRRDRNAHGRVERFPAG
                     RAATDGRPPGAAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRSGGGRARRGARLPGDL
                     RAPQPPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     gene            984..1583
                     /gene="pac"
                     /label=pac
     regulatory      1883..1908
                     /note="terminator for Methanococcus voltae methyl coenzyme
                     m reductase"
                     /regulatory_class="terminator"
     promoter        2262..2366
                     /label=AmpR promoter
     CDS             2367..3224
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3398..3986
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4274..4295
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4310..4340
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4348..4364
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4372..4388
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttccaaaata taacataaat aacataggtt taaataattt aaaggcatat tttatataaa
       61 caattgtaaa atattggctt atgaaatttg ttaaaattta gctaataagc tattgatatt
      121 ataattttat agataactaa taggtgaatc atatgaagca tacgcgtctt aagagatctc
      181 atgatatcta gatcctctag aggatgatta attttaagag agtcttaaaa aatcttcgga
      241 aaatagtagt aggtaataaa aaacgcccta ttcgtaatat actattcaaa actaaatatt
      301 cattgaaata tttgggtaat aattaatatt aatctaatat ttagatattg agatataata
      361 ataacatttt tttaaaaaat catctatttt catcttaatt ttatatatta aagcccattt
      421 tttgagtatt caaattcaaa ttactgtgtt attaacatct tatatataaa cttttctatt
      481 taatgttaat gaaaaagtga atatatatac atagagtaat gttatgatgt atatatcaaa
      541 aaaataggag tgattcctat ggtaaagtat gaagataaga tatgtctatt tgatgcgaaa
      601 ggaaaccaag tagcagaaga tgtaccattg gaagctatca gcccattaaa taacccaact
      661 ataatgggga tggttaaaaa tattaaaaga accgttgctg ttgacggatc cccagcttgc
      721 atgcctgcag gtcgacatcg cggccgaacc ggttcgtgag cccctgggac atcgccgccc
      781 tccagatcct cgtggaggag gcgggcggcg tctgcaccga cctgctcggc ggctcgcccc
      841 agcgcgggac gggtgccctg tccgccaacc cggagctgca ccggctcgcc gtggaggccc
      901 tcgcgccccc gcggccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac
      961 cccacacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     1021 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     1081 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt cacccgagct gcaagaactc
     1141 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcgccaca cggcgccgcg
     1201 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcgga
     1261 acgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     1321 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     1381 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     1441 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc ttctacgagc
     1501 ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca
     1561 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg agccgaaagg
     1621 agcgcacgac cccatggctc cgaccgaagc cgacccgggc ggccccgccg accccgcacc
     1681 cgcccccgag gcccaccgcg ggggacacac cgaacacgcc gaccctgctg aacacgcggc
     1741 gcagttcggt gcccaggagc ggatcgggaa ttaattcgaa gctgctggtg aaagagaccc
     1801 tatcttacct gctaaaatct aagttaatta ctaatttatt attaatttat tattagattg
     1861 ggcaaaatag taaaagaaaa ctaaaggaaa cctaatatgg tttccttttt ttatatattt
     1921 ttaattcact ggggggattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1981 cttggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     2041 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     2101 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     2161 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2221 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2281 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2341 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2401 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2461 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2521 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2581 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2641 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2701 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2761 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2821 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2881 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2941 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3001 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3061 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3121 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3181 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3241 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3541 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4021 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4081 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4141 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4201 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4261 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4321 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4381 gctatgacca tgattacgaa
//
