LOCUS       40924_46573        4260 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative vector pWLG11, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Expression vectors for Methanococcus maripaludis: overexpression of 
            acetohydroxyacid synthase and beta-galactosidase
  JOURNAL   Genetics 152 (4), 1439-1447 (1999)
  PUBMED    10430574
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Gardner WL, Whitman WB.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-1999) Microbiology, University of Georgia, 
            Biological Sciences Building, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 4260)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1999"; volume: "152"; issue: "4"; pages: "1439-1447"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-1999) Microbiology, University of Georgia, Biological 
            Sciences Building, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..61
                     /label=polylinker
                     /note="polylinker"
     regulatory      320..327
                     /note="Pmcr Box A; promoter for Methanococcus voltae methyl
                     coenzyme M reductase"
                     /regulatory_class="promoter"
     CDS             844..1443
                     /codon_start=1
                     /gene="pac"
                     /product="puromycin transacetylase"
                     /label=pac
                     /note="from Streptomyces alboniger"
                     /protein_id="AAF01142.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTRAARTLPHARRARHRQGVGRATRRRGGGLDHAGERRSGGGVRRDRNAHGRVERFPAG
                     RAATDGRPPGAAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRSGGGRARRGARLPGDL
                     RAPQPPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     gene            844..1443
                     /gene="pac"
                     /label=pac
     regulatory      1743..1768
                     /note="terminator for Methanococcus voltae methyl coenzyme
                     m reductase"
                     /regulatory_class="terminator"
     promoter        2122..2226
                     /label=AmpR promoter
     CDS             2227..3084
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3258..3846
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4134..4155
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4170..4200
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4208..4224
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4232..4248
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcaagcatc atatgaagca tacgcgtctt aagagatctc atgatatcta gatcctctag
       61 aggatgatta attttaagag agtcttaaaa aatcttcgga aaatagtagt aggtaataaa
      121 aaacgcccta ttcgtaatat actattcaaa actaaatatt cattgaaata tttgggtaat
      181 aattaatatt aatctaatat ttagatattg agatataata ataacatttt tttaaaaaat
      241 catctatttt catcttaatt ttatatatta aagcccattt tttgagtatt caaattcaaa
      301 ttactgtgtt attaacatct tatatataaa cttttctatt taatgttaat gaaaaagtga
      361 atatatatac atagagtaat gttatgatgt atatatcaaa aaaataggag tgattcctat
      421 ggtaaagtat gaagataaga tatgtctatt tgatgcgaaa ggaaaccaag tagcagaaga
      481 tgtaccattg gaagctatca gcccattaaa taacccaact ataatgggga tggttaaaaa
      541 tattaaaaga accgttgctg ttgacggatc cccagcttgc atgcctgcag gtcgacatcg
      601 cggccgaacc ggttcgtgag cccctgggac atcgccgccc tccagatcct cgtggaggag
      661 gcgggcggcg tctgcaccga cctgctcggc ggctcgcccc agcgcgggac gggtgccctg
      721 tccgccaacc cggagctgca ccggctcgcc gtggaggccc tcgcgccccc gcggccgcca
      781 cgaccggtgc cgccaccatc ccctgaccca cgcccctgac cccacacaag gagacgacct
      841 tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
      901 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
      961 gaccgccaca tcgagcgggt cacccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1021 cgacatcggc aaggtgtggg tcgcgccaca cggcgccgcg gtggcggtct ggaccacgcc
     1081 ggagagcgtc gaagcggggg cggtgttcgc cgagatcgga acgcgcatgg ccgagttgag
     1141 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1201 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1261 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     1321 cctggagacc tccgcgcccc gcaacctccc ttctacgagc ggctcggctt caccgtcacc
     1381 gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc
     1441 tgacgcccgc cccacgaccc gcagcgcccg agccgaaagg agcgcacgac cccatggctc
     1501 cgaccgaagc cgacccgggc ggccccgccg accccgcacc cgcccccgag gcccaccgcg
     1561 ggggacacac cgaacacgcc gaccctgctg aacacgcggc gcagttcggt gcccaggagc
     1621 ggatcgggaa ttaattcgaa gctgctggtg aaagagaccc tatcttacct gctaaaatct
     1681 aagttaatta ctaatttatt attaatttat tattagattg ggcaaaatag taaaagaaaa
     1741 ctaaaggaaa cctaatatgg tttccttttt ttatatattt ttaattcact ggggggattt
     1801 aaaacttcat ttttaattta aaaggatcta ggtgaagatc cttggtgcac tctcagtaca
     1861 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     1921 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1981 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     2041 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2941 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3961 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4021 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4081 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4141 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     4201 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa
//
