LOCUS       40924_46558        9017 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWIZdir, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Modified vectors for the two-step directional cloning of inverted 
            repeats for RNA interference in Drosophila
  JOURNAL   BioTechniques 44 (3), 335-339 (2008)
  PUBMED    18361787
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA
REFERENCE   3  (bases 1 to 9017)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9017)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2008"; volume: "44"; issue: "3"; pages: 
            "335-339"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3057..3151
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3170..3408
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          3468..3541
                     /note="white intron in hairpin"
     intron          3652..3717
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3847..3867
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4139..4273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4293..8421
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(8432..9017)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg agatccaagc ttgcatgcct gcaggtcgga
     3061 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3121 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa
     3181 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     3241 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     3301 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     3361 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
     3421 ggaattcgtt aacagatctg cggccgcggc tcgagactag tgcctaggtg agtttctatt
     3481 cgcagtcggc tgatctgtgt gaaatcttaa tgaagggtcc aattaccaat ttgaaactca
     3541 gctagctcta gaatatcaat tgggatccac tagaggatct ttgtgaagga accttacttc
     3601 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     3661 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     3721 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     3781 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     3841 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     3901 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     3961 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt gatgtatagt
     4021 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4081 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4141 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4201 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4261 tcatgtctgg atctccacta gaaggcctaa ttctagtatg tatgtaagtt aataaaaccc
     4321 atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg
     4381 aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct
     4441 ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc
     4501 tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc
     4561 gtttgtagcg ttacctagcg tcaatgtccg ccttcagttg cactttgtca gcggtttcgt
     4621 gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct
     4681 ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg
     4741 taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat
     4801 tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc
     4861 atacatctta agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg
     4921 cgtgagaact gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa tagtgacacg
     4981 aaacagatta ttctggtagc tgtgctcgct atataagaca atttttaaga tcatatcatg
     5041 atcaagacat ctaaaggcat tcattttcga ctacattctt ttttacaaaa aatataacaa
     5101 ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc
     5161 gtaggatact tcgttttgtt cggggttaga tgagcataac gcttgtagtt gatatttgag
     5221 atcccctatc attgcagggt gacagcggag cggcttcgca gagctgcatt aaccagggct
     5281 tcgggcaggc caaaaactac ggcacgctcc tgccacccag tccgccggag gactccggtt
     5341 cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg
     5401 gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat
     5461 tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc
     5521 tattcgcagt cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa
     5581 ctcagtttgc ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc
     5641 cggaaagacg accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc
     5701 gccatccggg atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag
     5761 gtgcgcctat gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct
     5821 gattttccag gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg
     5881 cgtggatcag gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt
     5941 gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga
     6001 ggcactaacc gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcatt
     6061 taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat
     6121 cctgaccatt catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat
     6181 ggccgagggc agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta
     6241 gtgagttcga tgtgtttatt aagggtatct agcattacat tacatctcaa ctcctatcca
     6301 gcgtgggtgc ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg
     6361 ccgttgtgcc cggacgggag atcgagtccc gtgatcggat cgccaagata tgcgacaatt
     6421 ttgctattag caaagtagcc cgggatatgg agcagttgtt ggccaccaaa aatttggaga
     6481 agccactgga gcagccggag aatgggtaca cctacaaggc cacctggttc atgcagttcc
     6541 gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga accactcctc gtaaaagtgc
     6601 gacttattca gacaacggtg agtggttcca gtggaaacaa atgatataac gcttacaatt
     6661 cttggaaaca aattcgctag attttagtta gaattgcctg attccacacc cttcttagtt
     6721 tttttcaatg agatgtatag tttatagttt tgcagaaaat aaataaattt catttaactc
     6781 gcgaacatgt tgaagatatg aatattaatg agatgcgagt aacattttaa tttgcagatg
     6841 gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt gggcgtgatg
     6901 aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc
     6961 acgataaatg taagtcttgt ttagaataca tttgcatatt aataatttac taactttcta
     7021 atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg
     7081 aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg aattaccgct
     7141 ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc
     7201 cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac
     7261 gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg
     7321 tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt
     7381 gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct
     7441 gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac
     7501 cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct
     7561 gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct
     7621 ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt
     7681 gttttttttt ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta
     7741 atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc
     7801 cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc
     7861 tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta
     7921 tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc
     7981 gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac
     8041 gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg
     8101 gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc
     8161 aatatatttg tatatatttc cctcacagct atatttattc taatttaata ttatgacttt
     8221 ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg
     8281 acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt
     8341 tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg
     8401 cttggatttc actggaacta gaattaattc ggctgctgct ctaaacgacg catttcgtac
     8461 tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta
     8521 acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt
     8581 ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt
     8641 actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc
     8701 agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct
     8761 gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa
     8821 ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac
     8881 gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc
     8941 aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct
     9001 tatgttattt catcatg
//
